Western Blot (WB)
(Western Blot analysis of RAD18 on different lysates using anti-RAD18 antibody at 1/1,000 dilution. Positive control: Lane 1: NIH/3T3 Lane 2: 293T Lane 3: MCF-7)
Rabbit RAD18 Polyclonal Antibody | anti-RAD18 antibody
RAD18 Polyclonal Antibody
Gene Names
Zyx; R75157; 9530098H06Rik
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Flow Cytometry, Functional Assay
Purity
ProA affinity purified
Synonyms
RAD18; Polyclonal Antibody; RAD18 Polyclonal Antibody; hHR18; hRAD18; 2810024C04Rik; DNA repair protein rad18; E3 ubiquitin-protein ligase RAD18; EC 6.3.2.-; hHR 18; hRAD 18; MGC156682; Postreplication repair E3 ubiquitin-protein ligase RAD18; Postreplication repair protein hRAD18p; Postreplication repair protein RAD18; RAD 18; RAD18 homolog (S. cerevisiae); RAD18 homolog; RAD18 S. cerevisiae homolog; RAD18 S. cerevisiae homolog of; RAD18 transcript variant; Rad18sc; Radiation sensitivity protein 18; RING finger protein 73; RNF 73; RNF73; Structural maintenance of chromosomes protein 6; YCR066W; YCR66W; anti-RAD18 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
RAD18 polyclonal antibody detects endogenous levels of RAD18 protein.
Purity/Purification
ProA affinity purified
Form/Format
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Applicable Applications for anti-RAD18 antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
Application Notes
WB: 1:1,000-1:2,000
ICC: 1:100-1:500
IHC: 1:50-1:200
FC/FACS: 1:50-1:100
ICC: 1:100-1:500
IHC: 1:50-1:200
FC/FACS: 1:50-1:100
Immunogen
recombinant protein
Preparation and Storage
Store at 4 degree C after thawing.
Aliquot store at -20 degree C or -80 degree C.
Avoid repeated freeze/thaw cycles.
Aliquot store at -20 degree C or -80 degree C.
Avoid repeated freeze/thaw cycles.
Western Blot (WB)
(Western Blot analysis of RAD18 on different lysates using anti-RAD18 antibody at 1/1,000 dilution. Positive control: Lane 1: NIH/3T3 Lane 2: 293T Lane 3: MCF-7)
Western Blot (WB)
(Western Blot analysis of RAD18 on different lysates using anti-RAD18 antibody at 1/1,000 dilution. Positive control: Lane 1: NIH/3T3 Lane 2: 293T Lane 3: MCF-7)
Immunocytochemistry (ICC)
(ICC staining RAD18 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
Immunocytochemistry (ICC)
(ICC staining RAD18 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
Related Product Information for anti-RAD18 antibody
The RING-type zinc finger protein Rad18 is essential in post-replication repair of damaged DNA and contributes to the maintenance of genomic stability. Rad18 maintains chromosomal DNA with the Rad54-dependent DNA repair pathway and recruits ubiquitin-conjugating enzymes in the Rad6 pathway. Rad18 functions in gap-filling of a daughter strand on replication of damaged DNA and is localized in the nucleus.
NCBI and Uniprot Product Information
NCBI GeneID
Molecular Weight
56/90kDa
NCBI Official Synonym Full Names
zyxin
NCBI Official Symbol
Zyx
NCBI Official Synonym Symbols
R75157; 9530098H06Rik
NCBI Protein Information
zyxin
Protein Family
Research Articles on RAD18
Similar Products
Product Notes
The RAD18 (Catalog #AAA3013332) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The RAD18 Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's RAD18 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS). WB: 1:1,000-1:2,000 ICC: 1:100-1:500 IHC: 1:50-1:200 FC/FACS: 1:50-1:100. Researchers should empirically determine the suitability of the RAD18 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RAD18, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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