Immunofluorescence (IF)
(Immunofluorescence analysis of A549 cells, using RAD Antibody. The picture on the right is blocked with the synthesized peptide.)
Rabbit anti-Human RAD Polyclonal Antibody | anti-RAD antibody
RAD Antibody
Gene Names
RRAD; RAD; RAD1; REM3
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, ELISA
Purity
Purified from rabbit antiserum by affinity-chromatography using immunogen.
Synonyms
RAD; Polyclonal Antibody; RAD Antibody; GTP-binding protein RAD; RAS associated with diabetes; RAD1; anti-RAD antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Specificity
RAD Antibody detects endogenous levels of total RAD protein.
Purity/Purification
Purified from rabbit antiserum by affinity-chromatography using immunogen.
Form/Format
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1 mg/ml (varies by lot)
Sequence Length
308
Applicable Applications for anti-RAD antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA (EIA)
Application Notes
WB: 1:500~1:1000
IHC: 1:50~1:100
IF: 1:100~1:500
ELISA: 1:20000
IHC: 1:50~1:100
IF: 1:100~1:500
ELISA: 1:20000
Target Modification
Unmodified/Total
Immunogen
The antiserum was produced against synthesized peptide derived from human RAD.
Immunogen Range
41-90
Preparation and Storage
Stable at -20 degree C for at least 1 year.
Immunofluorescence (IF)
(Immunofluorescence analysis of A549 cells, using RAD Antibody. The picture on the right is blocked with the synthesized peptide.)
Immunofluorescence (IF)
(Immunofluorescence analysis of A549 cells, using RAD Antibody. The picture on the right is blocked with the synthesized peptide.)
Immunohistochemistry (IHC)
(Immunohistochemistry analysis of paraffin-embedded human heart tissue, using RAD Antibody. The picture on the right is blocked with the synthesized peptide.)
Immunohistochemistry (IHC)
(Immunohistochemistry analysis of paraffin-embedded human heart tissue, using RAD Antibody. The picture on the right is blocked with the synthesized peptide.)
Western Blot (WB)
(Western blot analysis of lysates from HeLa and HepG2/MCF-7 cells, using RAD Antibody. The lane on the right is blocked with the synthesized peptide.)
Western Blot (WB)
(Western blot analysis of lysates from HeLa and HepG2/MCF-7 cells, using RAD Antibody. The lane on the right is blocked with the synthesized peptide.)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
33 kDa
NCBI Official Full Name
GTP-binding protein RAD
NCBI Official Synonym Full Names
RRAD, Ras related glycolysis inhibitor and calcium channel regulator
NCBI Official Symbol
RRAD
NCBI Official Synonym Symbols
RAD; RAD1; REM3
NCBI Protein Information
GTP-binding protein RAD
UniProt Protein Name
GTP-binding protein RAD
Protein Family
UniProt Gene Name
RRAD
UniProt Synonym Gene Names
RAD
Uniprot Description
May play an important role in cardiac antiarrhythmia via the strong suppression of voltage-gated L-type Ca2+ currents. Regulates voltage-dependent L-type calcium channel subunit alpha-1C trafficking to the cell membrane (). Inhibits cardiac hypertrophy through the calmodulin-dependent kinase II (CaMKII) pathway. Inhibits phosphorylation and activation of CAMK2D.
Research Articles on RAD
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Product Notes
The RAD rrad (Catalog #AAA9506432) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The RAD Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's RAD can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA (EIA). WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:20000. Researchers should empirically determine the suitability of the RAD rrad for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RAD, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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