Rabbit anti Swine secretory IgA Secondary Antibody
Rabbit anti Swine secretory IgA
Reactivity
Swine
Applications
Immunoprecipitation
Synonyms
Rabbit anti Swine secretory IgA; Polyclonal Secondary Antibody; Rabbit anti Swine secretory IgA secondary antibody
Host
Rabbit
Reactivity
Swine
Clonality
Polyclonal
Specificity
Precipitating polyclonal Rabbit antiSerum to Swine secretory IgA.
Applicable Applications for Rabbit anti Swine secretory IgA secondary antibody
Immunoprecipitation (IP)
Application Notes
Immunoprecipitation. In immunoelectrophoresis use 2 ul serum or equivalent against 120 ul antiserum. In double radial immunodiffusion (Ouchterlony) use a rosette arrangement with 10 ul antiserum in 3 mm diameter center well and 2 ul serum samples (neat and serially diluted in 2 mm diameter peripheral wells.
Source
Highly purified secretory IgA isolated from pooled swine milk. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Product
Delipidated, heat inactivated, lyophilized, stable whole antiserum. No preservative added. Total protein and IgG concentrations in the antiserum are comparable to those of pooled normal rabbit serum. No foreign proteins added.
Reactivity Note
The antiSerum does not cross-react with any other component of the Swine immunoglobulin system. Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins, since Ig of different species frequently share antigenic determinants. Cross-reactivity of this antiSerum has not been tested in detail
Preparation and Storage
The lyophilized antiserum is shipped at ambient temperature and may be stored at +4 degree C; prolonged storage at or below -20 degree C. Reconstitute the lyophilized antiserum by adding 1 ml sterile distilled water. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the antiserum. Diluted antiserum should be stored at +4 degree C, not refrozen, and preferably used the same day. Lyophilized at +4 degree C--at least 10 years. Reconstituted at or below -20 degree C--3-5 years. Reconstituted at +4 degree C--7 days
Related Product Information for Rabbit anti Swine secretory IgA secondary antibody
The reactivity of the antiserum is restricted to the Fc part of the IgA molecule and the surface and hidden determinants of the secretory component. In immunoelectrophoresis and radial immunodiffusion, using various antiserum concentrations against normal swine milk two precipitin lines may be obtained, one representing IgA and the other the free secretory component. Against normal swine serum only IgA is precipitated. No precipitation reaction is obtained with purified IgG, IgM, and IgG/Fab fragments. In precipitating techniques as immunoelectrophoresis and radial immunodiffusion to identify the presence of IgA in swine milk or other body fluids or to determine its concentration. To prepare an immunoadsorbent for the purification of swine IgA from serum or exocrine secretions. Antisera to IgA or sIgA do not discriminate between serum IgA (monomeric and dimeric) and higher molecular forms such as secretory IgA. This antiserum is not intended for use in non-precipitating antibody-binding or other highly sensitive assays. This does not exclude the use of the antiserum in more sensitive techniques if proper controls are included.
Product Categories/Family for Rabbit anti Swine secretory IgA secondary antibody
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Product Notes
The Rabbit anti Swine secretory IgA (Catalog #AAA571326) is a Secondary Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti Swine secretory IgA reacts with Swine and may cross-react with other species as described in the data sheet. AAA Biotech's Rabbit anti Swine secretory IgA can be used in a range of immunoassay formats including, but not limited to, Immunoprecipitation (IP). Immunoprecipitation. In immunoelectrophoresis use 2 ul serum or equivalent against 120 ul antiserum. In double radial immunodiffusion (Ouchterlony) use a rosette arrangement with 10 ul antiserum in 3 mm diameter center well and 2 ul serum samples (neat and serially diluted in 2 mm diameter peripheral wells. Researchers should empirically determine the suitability of the Rabbit anti Swine secretory IgA for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Rabbit anti Swine secretory IgA, Polyclonal Secondary Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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