Rabbit anti Monkey IgG Fab Secondary Antibody

Rabbit anti Monkey IgG Fab

Cat. Num. AAA571335

• Reactivity: Monkey
• Applications: Immunofluorescence, ELISA, Dot Blot, Immunoblot, Western Blot
• Purity: Hyperimmune antisera with strong precipitating activity are selected for fractionation by saltprecipitation and purification of the IgG fraction by DEAE-chromatography.
• Synonyms: Rabbit anti Monkey IgG Fab; Polyclonal Secondary Antibody; Rabbit anti Monkey IgG Fab secondary antibody

• Host: Rabbit
• Reactivity: Monkey
• Clonality: Polyclonal
• Specificity: Purified IgG fraction of polyclonal Rabbit antiSerum to Monkey Fab of IgG
• Purity/Purification: Hyperimmune antisera with strong precipitating activity are selected for fractionation by saltprecipitation and purification of the IgG fraction by DEAE-chromatography.
• Form/Format: Purified hyperimmune rabbit IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2).

• Applicable Applications for Rabbit anti Monkey IgG Fab secondary antibody: Indirect Immunofluorescence (IF), ELISA (EIA), Dot Blot (DB), Immunoblotting (IB)
• Application Notes: Typical working dilutions in histochemistry are usually between 1:50 and 1:500; In ELISA and comparable non-precipitating antibody-binding assays between 1:1000 and 1:5000; ; Intended use: For indirect staining or fixed celland tissue substrates , to demonstrate the intracellular presence of free or Ig-bound subunits of both kappa and lambda type. In general this kind of products is not recommended as direct or indirect screening reagents for immunoglobulin isotypes on the surface of membranes of vital lymphoid cells. The presence of activity to the common Fab subunit may result in the staining of Ig bound to Fc-receptors on non-lymphoid cells. Combinations of isotype-specific reagents should be used instead for this purpose. When applied in any cytochemical or histochemical procedure or solids phase coupling technique, the optimum concentration of the IgG preparation should always be established by titration.
• Immunogen: Purified Fab from normal IgG isolated from pooled Rhesus monkey serum. Freund's complete adjuvant is used in the first step of the immunization procedure.
• Absorption: Immunoaffinity adsorbed using insolubilized antigens as required to eliminate antibodies reacting with other subunits of the immunoglobulin or reacting with other serum proteins. The use of insolubilized adsorption antigens prevents the presence of foreign protein or immune complexes in the antiserum.
• Reconstitution: It is reconstituted by adding 1 ml sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20°C.
• Preservative: No preservative added, as it may interfere with the antibody activity. No foreign protein added.
• Identity and Specifity: The antiserum is reacting with the Fab subunit of intact IgG, IgA and IgM and other Ig classes of both light chain types, with their Fab or F(ab’)2 subunits and with free light chains of kappa and lambda type as tested in immunoelectrophoresis and double radial immunodiffusion.
• Physochemical characteristics: IgG protein concentration 10 mg/mL.No foreign proteins added.
• Antibody titre: Precipitin tire not less than 1:64 when tested againsnt normal monkey serum in agar block titration.
• Cross-reactivity: Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins, since Ig of different species frequently share antigenic determinants. Cross-reactivity of this antiSerum has not been tested in detail, however in immunoelectrophoresis a reaction with Human has been observed.
• Preparation and Storage: Store lyophilized at 4°C. After reconstitution , store at 4°C for one week or at -20°C for long term.

Related Product Information for Rabbit anti Monkey IgG Fab secondary antibody

The antiserum is reacting with the Fab subunit of intact IgG, IgA and IgM and other Ig classes of both light chain types, with their Fab or F(ab’)2 subunits and with free light chains of kappa and lambda type as tested in immunoelectrophoresis and double radial immunodiffusion. For indirect staining of fixed cell and tissue substrates, to demonstrate the intracellular presence of free or Ig-bound subunits of both kappa and lambda type. In general this kind of products is not recommended as direct or indirect screening reagents for immunoglobulin isotypes on the surface of membranes of vital lymphoid cells. The presence of activity to the common Fab subunit may result in the staining of Ig bound to Fc-receptors on non-lymphoid cells. Combinations of isotype-specific reagents should be used instead for this purpose. When applied in any cytochemical or histochemical procedure or solids phase coupling technique, the optimum concentration of the IgG preparation should always be established by titration.

Product Categories/Family for Rabbit anti Monkey IgG Fab secondary antibody

Secondary Antibody