Western Blot (WB)
(Western blot analysis of Rab9 expression in rat brain extract (lane 1), mouse brain extract (lane 2), HELA whole cell lysates (lane 3) and MCF-7 whole cell lysates (lane 4). Rab9 at 23KD was detected using rabbit anti- Rab9 Antigen Affinity purified polyclonal antibody at0.5 ug/mL. The blot was developed using chemiluminescence (ECL) method. )
Rab9 Polyclonal Antibody | anti-Rab9 antibody
Anti-Rab9 Antibody
Gene Names
RAB9A; RAB9
Reactivity
Human, Mouse, Rat
Applications
Western Blot
Purity
Immunogen Affinity Purified
Synonyms
Rab9; Polyclonal Antibody; Anti-Rab9 Antibody; Ras-related protein Rab-9A; 2410064E05Rik; AI195561; DmRab9; Rab 9; RAB 9A; RAB9 member RAS oncogene family; RAB9A; RAB9A member RAS oncogene family; RAS ASSOCIATED PROTEIN RAB9; Ras related protein Rab 9A; Sid6061p; Sid99; member RAS oncogene family; anti-Rab9 antibody
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Purity/Purification
Immunogen Affinity Purified
Form/Format
Lyophilized. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Sequence Length
201
Applicable Applications for anti-Rab9 antibody
Western Blot (WB)
Application Notes
Western Blot Concentration: 0.1-0.5ug/ml
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human Rab9 (156-193aa KDATNVAAAFEEAVRRVLATEDRSDHLIQTDTVNLHRK), different from the related mouse sequence by three amino acids, and from the related rat sequence by two amino acids.
Ig Type
Rabbit IgG
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Preparation and Storage
At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquoted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
Western Blot (WB)
(Western blot analysis of Rab9 expression in rat brain extract (lane 1), mouse brain extract (lane 2), HELA whole cell lysates (lane 3) and MCF-7 whole cell lysates (lane 4). Rab9 at 23KD was detected using rabbit anti- Rab9 Antigen Affinity purified polyclonal antibody at0.5 ug/mL. The blot was developed using chemiluminescence (ECL) method. )
Western Blot (WB)
(Western blot analysis of Rab9 expression in rat brain extract (lane 1), mouse brain extract (lane 2), HELA whole cell lysates (lane 3) and MCF-7 whole cell lysates (lane 4). Rab9 at 23KD was detected using rabbit anti- Rab9 Antigen Affinity purified polyclonal antibody at0.5 ug/mL. The blot was developed using chemiluminescence (ECL) method. )
Related Product Information for anti-Rab9 antibody
Description: Rabbit IgG polyclonal antibody for Ras-related protein Rab-9A(RAB9A) detection. Tested with WB in Human;Mouse;Rat.
Background: Ras-related protein Rab-9A, also called RAB9, is a protein that in humans is encoded by the RAB9A gene. This gene is mapped to Xp22.2. RAB9 has been localized to components of the endocytic/exocytic pathway and has been implicated in recycling of membrane receptors. It has been found that downregulation of RAB9A gene expression in HeLa cells induced severe cell vacuolation. RAB9A GTPase is directly bound by TIP47 in its active, GTP-bound conformation. Moreover, RAB9A increases the affinity of TIP47 for its cargo. What's more, this gene may involved in the transport of proteins between the endosomes and the trans Golgi network. RAB9A has been shown to interact with RABEPK and TIP47.
Background: Ras-related protein Rab-9A, also called RAB9, is a protein that in humans is encoded by the RAB9A gene. This gene is mapped to Xp22.2. RAB9 has been localized to components of the endocytic/exocytic pathway and has been implicated in recycling of membrane receptors. It has been found that downregulation of RAB9A gene expression in HeLa cells induced severe cell vacuolation. RAB9A GTPase is directly bound by TIP47 in its active, GTP-bound conformation. Moreover, RAB9A increases the affinity of TIP47 for its cargo. What's more, this gene may involved in the transport of proteins between the endosomes and the trans Golgi network. RAB9A has been shown to interact with RABEPK and TIP47.
References
1. Carroll, K. S., Hanna, J., Simon, I., Krise, J., Barbero, P., Pfeffer, S. R. Role of Rab9 GTPase in facilitating receptor recruitment by TIP47. Science 292: 1373-1376, 2001. 2. Davies, J. P., Cotter, P. D., Ioannou, Y. A. Cloning and mapping of human Rab7 and Rab9 cDNA sequences and identification of a Rab9 pseudogene. Genomics 41: 131-134, 1997.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
22,838 Da
NCBI Official Full Name
ras-related protein Rab-9A
NCBI Official Synonym Full Names
RAB9A, member RAS oncogene family
NCBI Official Symbol
RAB9A
NCBI Official Synonym Symbols
RAB9
NCBI Protein Information
ras-related protein Rab-9A
UniProt Protein Name
Ras-related protein Rab-9A
Protein Family
UniProt Gene Name
RAB9A
UniProt Synonym Gene Names
RAB9
UniProt Entry Name
RAB9A_HUMAN
Uniprot Description
RAB9A: Involved in the transport of proteins between the endosomes and the trans Golgi network. Interacts (preferentially in its GTP-bound form) with GCC2 (via its GRIP domain). Belongs to the small GTPase superfamily. Rab family.
Protein type: G protein; G protein, monomeric; G protein, monomeric, Rab
Chromosomal Location of Human Ortholog: Xp22.2
Cellular Component: cytosol; endoplasmic reticulum membrane; late endosome; lysosome; phagocytic vesicle; phagocytic vesicle membrane; plasma membrane; trans-Golgi network membrane; transport vesicle
Molecular Function: GDP binding; GTP binding; GTPase activity; protein binding
Biological Process: intracellular protein transport; metabolic process; negative regulation by host of symbiont molecular function; nucleocytoplasmic transport; positive regulation of exocytosis; regulation of protein localization; retrograde transport, endosome to Golgi; small GTPase mediated signal transduction
Research Articles on Rab9
1. Rab9-complexed TIP47 plays an essential role for the proper release of hepatitis C viral particles.
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Product Notes
The Rab9 rab9a (Catalog #AAA178044) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. The Anti-Rab9 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Rab9 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Western Blot Concentration: 0.1-0.5ug/ml. Researchers should empirically determine the suitability of the Rab9 rab9a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Rab9, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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