Rabbit RAB8A Polyclonal Antibody | anti-RAB8A antibody

RAB8A Antibody

Cat. Num. AAA7129579

• Gene Names: RAB8A; MEL; RAB8
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Antigen Affinity Purification
• Synonyms: RAB8A; Polyclonal Antibody; RAB8A Antibody; member RAS oncogene family; anti-RAB8A antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Antigen Affinity Purification
• Form/Format: Liquid; In Phosphate Buffered Saline, pH 7.4, 0.05% NaN3, and 40% Glycerol

• Applicable Applications for anti-RAB8A antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: ELISA: 1:2000-1:5000; WB: 1:500-1:2000; IHC: 1:25-1:100
• Immunogen: Synthetic peptide of human RAB8A
• Immunogen Species: Human
• Conjugate: Non-conjugated
• Preparation and Storage: Upon receipt, store at -20 degree C or -80 degree C. Avoid repeated freeze.

Immunohistochemistry (IHC)-Paraffin

(The image on the left is immunohistochemistry of paraffin-embedded Human brain tissue using MBS7129579(RAB8A Antibody) at dilution 1/45, on the right is treated with synthetic peptide. (Original magnification: ×200))

SDS-Page

(Gel: 12%SDS-PAGE, Lysate: 40 ug, Lane 1-3: Hela cells, hepg2 cells, Raw264.7 cells, Primary antibody: MBS7129579(RAB8A Antibody) at dilution 1/650, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 1 minute)

Product Categories/Family for anti-RAB8A antibody

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 46810392
• NCBI GeneID: 4218
• UniProt Accession #: P61006; P24407; Q6FHV5; B4DEK7
• Molecular Weight: 207
• NCBI Official Full Name: Ras-related protein Rab-8A
• NCBI Official Synonym Full Names: RAB8A, member RAS oncogene family
• NCBI Official Symbol: RAB8A
• NCBI Official Synonym Symbols: MEL; RAB8
• NCBI Protein Information: ras-related protein Rab-8A; oncogene c-mel; ras-associated protein RAB8; mel transforming oncogene (RAB8 homolog); mel transforming oncogene (derived from cell line NK14); mel transforming oncogene (derived from cell line NK14)- RAB8 homolog
• UniProt Protein Name: Ras-related protein Rab-8A
• Protein Family: Ras-related protein
• UniProt Gene Name: RAB8A
• UniProt Synonym Gene Names: MEL; RAB8
• UniProt Entry Name: RAB8A_HUMAN

NCBI Description

The protein encoded by this gene is a member of the RAS superfamily which are small GTP/GDP-binding proteins with an average size of 200 amino acids. The RAS-related proteins of the RAB/YPT family may play a role in the transport of proteins from the endoplasmic reticulum to the Golgi and the plasma membrane. This protein shares 97%, 96%, and 51% similarity with the dog RAB8, mouse MEL, and mouse YPT1 proteins, respectively and contains the 4 GTP/GDP-binding sites that are present in all the RAS proteins. The putative effector-binding site of this protein is similar to that of the RAB/YPT proteins. However, this protein contains a C-terminal CAAX motif that is characteristic of many RAS superfamily members but which is not found in YPT1 and the majority of RAB proteins. Although this gene was isolated as a transforming gene from a melanoma cell line, no linkage between MEL and malignant melanoma has been demonstrable. This oncogene is located 800 kb distal to MY09B on chromosome 19p13.1. [provided by RefSeq, Jul 2008]

Uniprot Description

RAB8A: May be involved in vesicular trafficking and neurotransmitter release. Together with RAB11A, RAB3IP, the exocyst complex, PARD3, PRKCI, ANXA2, CDC42 and DNMBP promotes transcytosis of PODXL to the apical membrane initiation sites (AMIS), apical surface formation and lumenogenesis. Together with MYO5B and RAB11A participates in epithelial cell polarization. Interacts with MAP4K2 and SYTL4. Interacts with SGSM1 and SGSM3. Interacts with OPTN. Interacts with RAB3IP. Interacts with MYO5B. Interacts with PIFO/C1orf88. Interacts with BIRC6/bruce. Interacts with OCRL. Belongs to the small GTPase superfamily. Rab family.

Protein type: G protein; Motility/polarity/chemotaxis; G protein, monomeric, Rab; G protein, monomeric; Oncoprotein

Chromosomal Location of Human Ortholog: 19p13.1

Cellular Component: centrosome; cytoplasmic vesicle membrane; secretory granule membrane; recycling endosome membrane; nonmotile primary cilium; dendrite; postsynaptic density; phagocytic vesicle; cytosol; cilium; nucleoplasm; Golgi membrane; synaptic vesicle; trans-Golgi network transport vesicle; phagocytic vesicle membrane; cell soma; plasma membrane; nucleolus; nucleus

Molecular Function: GTPase activity; protein binding; GDP binding; GTP binding; myosin V binding; protein kinase binding; Rab GTPase binding

Biological Process: regulation of long-term neuronal synaptic plasticity; metabolic process; organelle organization and biogenesis; protein secretion; regulation of protein transport; regulation of exocytosis; intracellular protein transport; synaptic vesicle exocytosis; axonogenesis; Golgi vesicle fusion to target membrane; cellular response to insulin stimulus; cilium biogenesis; mitotic cell cycle; Rab protein signal transduction; G2/M transition of mitotic cell cycle; vesicle docking during exocytosis