Rabbit anti-Human, Mouse RAB5B Polyclonal Antibody | anti-RAB5B antibody

RAB5B, ID (RAB5B, Ras-related protein Rab-5B) (FITC)

Cat. Num. AAA6339858

• Reactivity: Human, Mouse
• Applications: Western Blot
• Purity: Purified by Protein A and Peptide Affinity Chromatography.
• Synonyms: RAB5B; Polyclonal Antibody; ID (RAB5B; Ras-related protein Rab-5B) (FITC); Ras-related protein Rab-5B; anti-RAB5B antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human RAB5B. Species Crossreactivity: mouse
• Purity/Purification: Purified by Protein A and Peptide Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-RAB5B antibody: Western Blot (WB), FLISA
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 85-112 in the central region of human RAB5B Swiss/UniProt Accession: P61020. Species sequence homology: chicken
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in HepG2 cell line lysates (35ug/lane) usingMBS6008086. This demonstrates that MBS6008086 detected the RAB5B protein (arrow).)

Western Blot (WB)

(Western Blot analysis in mouse liver tissue lysates (35ug/lane) usingMBS6008086.This demonstrates that MBS6008086 detected the RAB5B protein (arrow).)

Product Categories/Family for anti-RAB5B antibody

Antibodies; GTPase; Rab; Ras; Rho Proteins

References

1. Jin, Y., et al. Nat. Genet. 42(7):576-578(2010). 2. Chen, P.I., et al. J. Biol. Chem. 284(44):30328-30338(2009). 3. Shin, N., et al. Exp. Cell Res. 314(10):2055-2065(2008). 4. Hakonarson, H., et al. Diabetes 57(4):1143-1146(2008). 5. Hirota, Y., et al. Biochem. Biophys. Res. Commun. 364(1):40-47(2007).

NCBI and Uniprot Product Information

• NCBI GI #: 4506371
• NCBI GeneID: 5869
• NCBI Accession #: NP_002859.1
• NCBI GenBank Nucleotide #: NM_002868.4
• UniProt Accession #: P61020; P35239; P35277; Q6PIK9; Q86TH0; Q8IXL2; A8K982; B4DKD7
• Molecular Weight: 19,090 Da
• NCBI Official Full Name: ras-related protein Rab-5B isoform 1
• NCBI Official Synonym Full Names: RAB5B, member RAS oncogene family
• NCBI Official Symbol: RAB5B
• NCBI Protein Information: ras-related protein Rab-5B
• UniProt Protein Name: Ras-related protein Rab-5B
• Protein Family: Ras-related protein
• UniProt Gene Name: RAB5B
• UniProt Entry Name: RAB5B_HUMAN

Uniprot Description

RAB5B: Protein transport. Probably involved in vesicular traffic. Belongs to the small GTPase superfamily. Rab family.

Protein type: G protein; G protein, monomeric, Rab; G protein, monomeric

Chromosomal Location of Human Ortholog: 12q13

Cellular Component: membrane; endocytic vesicle; early endosome membrane; early endosome; plasma membrane; melanosome; intracellular; endosome

Molecular Function: GTPase activity; protein binding; GDP binding; GTP binding; GTP-dependent protein binding

Biological Process: antigen processing and presentation; intracellular protein transport; endosome organization and biogenesis; metabolic process; regulation of endocytosis; plasma membrane to endosome transport; Rab protein signal transduction