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Western Blot (WB)
(Western blot analysis of Rab5 Antibody (C-term) in HL-60 cell line lysates (35ug/lane). RAB5 (arrow) was detected using the purified Pab.)
Rabbit anti-Human Rab5 Polyclonal Antibody | anti-Rab5 antibody
Rab5, CT (Ras-related Protein Rab-5A, RAB5A) (APC)
Gene Names
RAB5A; RAB5
Reactivity
Human
Applications
Flow Cytometry, Functional Assay, Immunofluorescence, Immunohistochemistry, Western Blot
Purity
Purified by Protein A Affinity Chromatography
Synonyms
Rab5; Polyclonal Antibody; CT (Ras-related Protein Rab-5A; RAB5A) (APC); anti-Rab5 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human Rab5.
Purity/Purification
Purified by Protein A Affinity Chromatography
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).
Applicable Applications for anti-Rab5 antibody
Flow Cytometry (FC/FACS), FLISA, Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB)
Application Notes
FC/FACS: 1:10-1:50
FLISA: 1:1000
IF: 1:10-1:50
IHC: 1:50-1:100
WB: 1:100-1:500
Applications are based on unconjugated antibody.
FLISA: 1:1000
IF: 1:10-1:50
IHC: 1:50-1:100
WB: 1:100-1:500
Applications are based on unconjugated antibody.
Immunogen
Synthetic peptide corresponding to a region near a portion within aa186-215 at the C-terminal region of human Rab5, conjugated to KLH.
Conjugate
APC
Note
Preservative Free
Special Handling
Light sensitive
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Western Blot (WB)
(Western blot analysis of Rab5 Antibody (C-term) in HL-60 cell line lysates (35ug/lane). RAB5 (arrow) was detected using the purified Pab.)
Western Blot (WB)
(Western blot analysis of Rab5 Antibody (C-term) in HL-60 cell line lysates (35ug/lane). RAB5 (arrow) was detected using the purified Pab.)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human brain tissue with Rab5 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human brain tissue with Rab5 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(Immunofluorescence analysis of Rab5 Antibody (C-term) with paraffin-embedded human brain tissue. 0.025 mg/ml primary antibody was followed by FITC-conjugated goat anti-rabbit lgG (whole molecule). FITC emits green fluorescence.Red counterstaining is PI.)
Immunohistochemistry (IHC)
(Immunofluorescence analysis of Rab5 Antibody (C-term) with paraffin-embedded human brain tissue. 0.025 mg/ml primary antibody was followed by FITC-conjugated goat anti-rabbit lgG (whole molecule). FITC emits green fluorescence.Red counterstaining is PI.)
Flow Cytometry (FC/FACS)
(Rab5 Antibody (C-term) flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Flow Cytometry (FC/FACS)
(Rab5 Antibody (C-term) flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Product Categories/Family for anti-Rab5 antibody
References
1. Farnsworth, C.C., et.al., Proc. Natl. Acad. Sci. USA 91(25), 11963-11967 (1994).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
215
NCBI Official Full Name
ras-related protein Rab-5A
NCBI Official Synonym Full Names
RAB5A, member RAS oncogene family
NCBI Official Symbol
RAB5A
NCBI Official Synonym Symbols
RAB5
NCBI Protein Information
ras-related protein Rab-5A; RAS-associated protein RAB5A
UniProt Protein Name
Ras-related protein Rab-5A
Protein Family
UniProt Gene Name
RAB5A
UniProt Synonym Gene Names
RAB5
UniProt Entry Name
RAB5A_HUMAN
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Product Notes
The Rab5 rab5a (Catalog #AAA6496343) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rab5, CT (Ras-related Protein Rab-5A, RAB5A) (APC) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Rab5 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), FLISA, Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB). FC/FACS: 1:10-1:50 FLISA: 1:1000 IF: 1:10-1:50 IHC: 1:50-1:100 WB: 1:100-1:500 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the Rab5 rab5a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Rab5, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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