Western Blot (WB)
(Western blot analysis of Rab4 expression in (1)MCF7 cell lysates (2)HeLa cell lysate.)
Rabbit Rab4 Polyclonal Antibody | anti-Rab4 antibody
Anti-Rab4 Rabbit pAb
Gene Names
RAB4A; RAB4; HRES1; HRES-1; HRES-1/RAB4
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunocytochemistry, Immunofluorescence, Flow Cytometry, Functional Assay
Purity
Affinity Chromatography
Synonyms
Rab4; Polyclonal Antibody; Anti-Rab4 Rabbit pAb; RAB4A; HRES 1; RAB4; Ras related protein Rab 4A; anti-Rab4 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Rab4 Antibody detects endogenous levels of total Rab4
Purity/Purification
Affinity Chromatography
Form/Format
Liquid; Phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Sequence Length
113
Applicable Applications for anti-Rab4 antibody
Western Blot (WB), Immunofluoresence/Immunocytochemistry (IF/ICC), Flow Cytometry (FC/FACS)
Application Notes
WB: 1:500-1:2000
ICC/IF: 1:50-1:200
FC/FACS: 1:50
ICC/IF: 1:50-1:200
FC/FACS: 1:50
Immunogen
A synthesized peptide derived from human Rab4
Cellular localization
Cytoplasm, Endosome, Membrane
Sequence Similarity
Belongs to the MS4A family.
Antibody Type
Primary Antibody
Conjugation
Unconjugated
Preparation and Storage
Store at 4 degree C for short term.
Aliquot and store at-20 degree C for long term.
Avoid freeze/thaw cycle.
Aliquot and store at-20 degree C for long term.
Avoid freeze/thaw cycle.
Western Blot (WB)
(Western blot analysis of Rab4 expression in (1)MCF7 cell lysates (2)HeLa cell lysate.)
Western Blot (WB)
(Western blot analysis of Rab4 expression in (1)MCF7 cell lysates (2)HeLa cell lysate.)
Immunofluorescence (IF)
(Immunofluorescent analysis of Hela cells, using Rab4 Antibody.)
Immunofluorescence (IF)
(Immunofluorescent analysis of Hela cells, using Rab4 Antibody.)
Related Product Information for anti-Rab4 antibody
The protein encoded by this gene belongs to the rab family. Rab4A protein is involved in the regulation of procathepsin L secretion, it was either inhibited by expression of a Rab4A dominant-negative mutant or increased by overexpression of the wild-type Rab4A in human melanoma cells.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
24kDa
NCBI Official Full Name
ras-related protein Rab-4A isoform 2
NCBI Official Synonym Full Names
RAB4A, member RAS oncogene family
NCBI Official Symbol
RAB4A
NCBI Official Synonym Symbols
RAB4; HRES1; HRES-1; HRES-1/RAB4
NCBI Protein Information
ras-related protein Rab-4A
UniProt Protein Name
Ras-related protein Rab-4A
Protein Family
UniProt Gene Name
RAB4A
UniProt Synonym Gene Names
RAB4
NCBI Description
This gene is a member of the largest group in the Ras superfamily of small GTPases, which regulate membrane trafficking. The encoded protein is associated with early endosomes and is involved in their sorting and recycling. The protein also plays a role in regulating the recycling of receptors from endosomes to the plasma membrane. Alternatively spliced transcript variants have been observed for this gene. [provided by RefSeq, Dec 2012]
Uniprot Description
Protein transport. Probably involved in vesicular traffic ().
Research Articles on Rab4
Similar Products
Product Notes
The Rab4 rab4a (Catalog #AAA477850) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-Rab4 Rabbit pAb reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Rab4 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluoresence/Immunocytochemistry (IF/ICC), Flow Cytometry (FC/FACS). WB: 1:500-1:2000 ICC/IF: 1:50-1:200 FC/FACS: 1:50. Researchers should empirically determine the suitability of the Rab4 rab4a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Rab4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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