Rabbit anti-Human RAB10 Polyclonal Antibody | anti-RAB10 antibody

RAB10, ID (RAB10, Ras-related protein Rab-10) (PE)

Cat. Num. AAA6339513

• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: RAB10; Polyclonal Antibody; ID (RAB10; Ras-related protein Rab-10) (PE); Ras-related protein Rab-10; anti-RAB10 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-RAB10 antibody: Flow Cytometry (FC/FACS), FLISA, Western Blot (WB)
• Application Notes: FC/FACS: 1:10-50; FLISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: RAB10 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 96-125 amino acids from the Central region of human RAB10.
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(RAB10 Antibody (Center) western blot analysis in A2058 cell line lysates (35ug/lane).This demonstrates the RAB10 antibody detected the RAB10 protein (arrow).)

Flow Cytometry (FC/FACS)

(RAB10 Antibody (Center) flow cytometric analysis of A2058 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-RAB10 antibody

RAB10 belongs to the RAS (see HRAS; MIM 190020) superfamily of small GTPases. RAB proteins localize to exocytic and endocytic compartments and regulate intracellular vesicle trafficking (Bao et al., 1998 [PubMed 9918381]).

Product Categories/Family for anti-RAB10 antibody

Antibodies; GTPase; Rab; Ras; Rho Proteins

References

Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010): Cardoso, C.M., et al. Traffic 11(2):221-235(2010). Roland, J.T., et al. J. Biol. Chem. 284(2):1213-1223(2009). Lamesch, P., et al. Genomics 89(3):307-315(2007). Ewing, R.M., et al. Mol. Syst. Biol. 3, 89 (2007).

NCBI and Uniprot Product Information

• NCBI GI #: 256222019
• NCBI GeneID: 10890
• NCBI Accession #: NP_057215.3
• NCBI GenBank Nucleotide #: NM_016131.4
• UniProt Accession #: P61026; O88386; Q6IA52; Q9D7X6; Q9H0T3; D6W538
• Molecular Weight: 22,541 Da
• NCBI Official Full Name: ras-related protein Rab-10
• NCBI Official Synonym Full Names: RAB10, member RAS oncogene family
• NCBI Official Symbol: RAB10
• NCBI Protein Information: ras-related protein Rab-10; GTP-binding protein RAB10; ras-related GTP-binding protein
• UniProt Protein Name: Ras-related protein Rab-10
• Protein Family: Ras-related protein
• UniProt Gene Name: RAB10
• UniProt Entry Name: RAB10_HUMAN

NCBI Description

RAB10 belongs to the RAS (see HRAS; MIM 190020) superfamily of small GTPases. RAB proteins localize to exocytic and endocytic compartments and regulate intracellular vesicle trafficking (Bao et al., 1998 [PubMed 9918381]).[supplied by OMIM, Mar 2009]

Uniprot Description

RAB10: a protein of the GTPase superfamily, Rab family. May be involved in vesicular trafficking and neurotransmitter release. Localized to the inner face of the cell membrane by a lipid-anchor.

Protein type: G protein, monomeric; G protein; G protein, monomeric, Rab

Chromosomal Location of Human Ortholog: 2p23.3

Cellular Component: Golgi apparatus; phagocytic vesicle membrane; endoplasmic reticulum membrane; recycling endosome; cytoplasmic vesicle membrane; focal adhesion; recycling endosome membrane; exocyst; plasma membrane; endosome membrane; trans-Golgi network; endosome

Molecular Function: GTPase activity; GDP binding; GTP binding; myosin V binding

Biological Process: metabolic process; establishment of neuroblast polarity; protein secretion; endosome transport; regulation of exocytosis; vesicle-mediated transport; polarized epithelial cell differentiation; intracellular protein transport; Golgi to plasma membrane transport; antigen processing and presentation; cellular response to insulin stimulus; axonogenesis; Golgi to plasma membrane protein transport; Rab protein signal transduction; vesicle docking during exocytosis