Western Blot (WB)
(QTRT1 Antibody (C-term) western blot analysis in SK-BR-3 cell line lysates (35ug/lane).This demonstrates the QTRT1 antibody detected the QTRT1 protein (arrow).)
Rabbit anti-Human QTRT1 Polyclonal Antibody | anti-QTRT1 antibody
QTRT1, CT (QTRT1, TGT, TGUT, Queuine tRNA-ribosyltransferase, Guanine insertion enzyme, tRNA-guanine transglycosylase) (FITC)
Gene Names
QTRT1; TGT; TGUT; FP3235
Reactivity
Human
Applications
Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
QTRT1; Polyclonal Antibody; CT (QTRT1; TGT; TGUT; Queuine tRNA-ribosyltransferase; Guanine insertion enzyme; tRNA-guanine transglycosylase) (FITC); tRNA-guanine transglycosylase; anti-QTRT1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).
Applicable Applications for anti-QTRT1 antibody
Western Blot (WB), FLISA
Application Notes
WB: 1:100-500
FLISA: 1:1,000
Applications are based on unconjugated antibody.
FLISA: 1:1,000
Applications are based on unconjugated antibody.
Immunogen
QTRT1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 344-371 amino acids from the C-terminal region of human QTRT1.
Conjugate
FITC
Note
Preservative Free
Special Handling
Light sensitive
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(QTRT1 Antibody (C-term) western blot analysis in SK-BR-3 cell line lysates (35ug/lane).This demonstrates the QTRT1 antibody detected the QTRT1 protein (arrow).)
Western Blot (WB)
(QTRT1 Antibody (C-term) western blot analysis in SK-BR-3 cell line lysates (35ug/lane).This demonstrates the QTRT1 antibody detected the QTRT1 protein (arrow).)
Related Product Information for anti-QTRT1 antibody
tRNA-guanine transglycosylase (TGT; EC 2.4.2.29) synthesizes queuosine (Q), which is found in tRNAs that recognize NAU and NAC codons, encoding tyr, asn, asp, and his. Prokaryotic TGT is a single protein of 43 kD. In contrast, mammalian TGT appears to be a heterodimer consisting of a 60-kD subunit (USP14; MIM 607274) and a 43-kD catalytic subunit (QTRT1) (Deshpande and Katze, 2001 [PubMed 11255023]).
Product Categories/Family for anti-QTRT1 antibody
References
Chen, Y.C., et al. RNA 16(5):958-968(2010). Deshpande, K.L., et al. Gene 265 (1-2), 205-212 (2001).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
24,015 Da
NCBI Official Full Name
queuine tRNA-ribosyltransferase
NCBI Official Synonym Full Names
queuine tRNA-ribosyltransferase catalytic subunit 1
NCBI Official Symbol
QTRT1
NCBI Official Synonym Symbols
TGT; TGUT; FP3235
NCBI Protein Information
queuine tRNA-ribosyltransferase
UniProt Protein Name
Queuine tRNA-ribosyltransferase
Protein Family
UniProt Gene Name
QTRT1
UniProt Synonym Gene Names
TGT; TGUT
UniProt Entry Name
TGT_HUMAN
Uniprot Description
QTRT1: Interacts with QTRTD1 to form an active queuine tRNA- ribosyltransferase. This enzyme exchanges queuine for the guanine at the wobble position of tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr), thereby forming the hypermodified nucleoside queuosine (Q) (7-(((4,5-cis-dihydroxy-2-cyclopenten-1- yl)amino)methyl)-7-deazaguanosine). Belongs to the queuine tRNA-ribosyltransferase family.
Protein type: EC 2.4.2.29; Mitochondrial; RNA processing; Transferase
Chromosomal Location of Human Ortholog: 19p13.3
Cellular Component: cytoplasm; mitochondrial outer membrane
Molecular Function: protein heterodimerization activity; protein homodimerization activity; queuine tRNA-ribosyltransferase activity
Biological Process: queuosine biosynthetic process; tRNA modification
Similar Products
Product Notes
The QTRT1 qtrt1 (Catalog #AAA6339473) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The QTRT1, CT (QTRT1, TGT, TGUT, Queuine tRNA-ribosyltransferase, Guanine insertion enzyme, tRNA-guanine transglycosylase) (FITC) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's QTRT1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), FLISA. WB: 1:100-500 FLISA: 1:1,000 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the QTRT1 qtrt1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "QTRT1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
