Rabbit anti-Human QPCT Polyclonal Antibody | anti-QPCT antibody

QPCT Polyclonal Antibody

Cat. Num. AAA9125720

• Gene Names: QPCT; QC; GCT; sQC
• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: Affinity Purification
• Synonyms: QPCT; Polyclonal Antibody; QPCT Polyclonal Antibody; QC; GCT; sQC; anti-QPCT antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Affinity Purification
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 361

• Applicable Applications for anti-QPCT antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: WB: 1:500 - 1:2000; IHC: 1:50 - 1:200; IF: 1:50 - 1:100
• Species: Human
• Immunogen: Recombinant Protein
• Immunogen: Recombinant protein of human QPCT
• Calculated Molecular Weight: 40kDa
• Preparation and Storage: Store at -20 degree C (regular) or -80 degree C (long term). Avoid freeze / thaw cycles.; Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Western Blot (WB)

(Western blot analysis of extracts of various cell lines, using QPCT antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 120s.)

Immunofluorescence (IF)

(Immunofluorescence analysis of MCF-7 cells using QPCT antibody. Blue: DAPI for nuclear staining.)

Related Product Information for anti-QPCT antibody

This gene encodes human pituitary glutaminyl cyclase, which is responsible for the presence of pyroglutamyl residues in many neuroendocrine peptides. The amino acid sequence of this enzyme is 86% identical to that of bovine glutaminyl cyclase.

Product Categories/Family for anti-QPCT antibody

Polyclonal

NCBI and Uniprot Product Information

• NCBI GI #: 2498824
• NCBI GeneID: 25797
• UniProt Accession #: Q16769; Q16770; Q3KRG6; Q53TR4
• Molecular Weight: 361
• NCBI Official Full Name: Glutaminyl-peptide cyclotransferase
• NCBI Official Synonym Full Names: glutaminyl-peptide cyclotransferase
• NCBI Official Symbol: QPCT
• NCBI Official Synonym Symbols: QC; GCT; sQC
• NCBI Protein Information: glutaminyl-peptide cyclotransferase; EC; glutamyl cyclase; glutaminyl cyclase; glutaminyl-tRNA cyclotransferase
• UniProt Protein Name: Glutaminyl-peptide cyclotransferase
• UniProt Gene Name: QPCT
• UniProt Synonym Gene Names: QC; sQC; EC
• UniProt Entry Name: QPCT_HUMAN

NCBI Description

This gene encodes human pituitary glutaminyl cyclase, which is responsible for the presence of pyroglutamyl residues in many neuroendocrine peptides. The amino acid sequence of this enzyme is 86% identical to that of bovine glutaminyl cyclase. [provided by RefSeq, Jul 2008]

Uniprot Description

QPCT: Responsible for the biosynthesis of pyroglutamyl peptides. Has a bias against acidic and tryptophan residues adjacent to the N-terminal glutaminyl residue and a lack of importance of chain length after the second residue. Also catalyzes N-terminal pyroglutamate formation. In vitro, catalyzes pyroglutamate formation of N-terminally truncated form of APP amyloid-beta peptides [Glu-3]-beta-amyloid. May be involved in the N-terminal pyroglutamate formation of several amyloid-related plaque-forming peptides. Belongs to the glutaminyl-peptide cyclotransferase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 2.3.2.5; Transferase; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 2p22.2

Molecular Function: glutaminyl-peptide cyclotransferase activity; zinc ion binding

Biological Process: peptidyl-pyroglutamic acid biosynthetic process, using glutaminyl-peptide cyclotransferase; protein modification process