Rabbit QIP1 / KPNA4 Polyclonal Antibody | anti-QIP1 antibody

Rabbit Polyclonal to Human QIP1 / KPNA4

Cat. Num. AAA243751

• Gene Names: KPNA4; QIP1; SRP3; IPOA3
• Reactivity: Human, Mouse, Rat
• Applications: Immunohistochemistry, Western Blot, ELISA
• Purity: Immunoaffinity Purified
• Synonyms: QIP1 / KPNA4; Polyclonal Antibody; Rabbit Polyclonal to Human QIP1 / KPNA4; Anti-QIP1 / KPNA4 Antibody (N-Terminus) IHC-plus; KPNA4; Importin subunit alpha-4; Importin alpha Q1; Karyopherin subunit alpha-4; SRP3; IPOA3; QIP1; Human QIP1; anti-QIP1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Specificity: Human KPNA4
• Purity/Purification: Immunoaffinity Purified
• Form/Format: PBS, 0.02% sodium azide
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 521

• Applicable Applications for anti-QIP1 antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
• Application Notes: ELISA, IHC-P (5 ug/ml), WB (1 - 2 ug/ml); Usage: KPNA4 antibody can be used for detection of KPNA4 by Western blot at 1-2 ug/ml.
• Target Species: Human
• Immunogen Description: A 15 amino acid peptide near the amino terminus of human KPNA4.
• Immunogen Type: Synthetic peptide
• Immunogen: QIP1 / KPNA4 antibody was raised against a 15 amino acid peptide near the amino terminus of human KPNA4.
• Antigen Modification: N-Terminus
• Preparation and Storage: Short term 4 degree C, long term aliquot and store at -20 degree C, avoid freeze thaw cycles. Store undiluted.

Immunohistochemistry (IHC)

(Anti-KPNA4 antibody IHC of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Immunohistochemistry (IHC)

(Anti-KPNA4 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Immunohistochemistry (IHC)

(Anti-KPNA4 antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Immunofluorescence (IF)

( )

Immunocytochemistry (ICC)

(Immunocytochemistry of KPNA4 in HeLa cells with KPNA4 antibody at 2.5 ug/ml.)

Western Blot (WB)

(Western blot analysis of KPNA1 in HeLa cell lysate with KPNA1 antibody at 1ug/ml.)

NCBI and Uniprot Product Information

• NCBI GI #: 4504901
• NCBI GeneID: 3840
• NCBI Accession #: NP_002259.1
• NCBI GenBank Nucleotide #: NM_002268.4
• Molecular Weight: 57,887 Da
• NCBI Official Full Name: importin subunit alpha-3
• NCBI Official Synonym Full Names: karyopherin alpha 4 (importin alpha 3)
• NCBI Official Symbol: KPNA4
• NCBI Official Synonym Symbols: QIP1; SRP3; IPOA3
• NCBI Protein Information: importin subunit alpha-3; importin alpha Q1; importin subunit alpha-4; karyopherin subunit alpha-4
• UniProt Protein Name: Importin subunit alpha-3
• UniProt Gene Name: KPNA4
• UniProt Synonym Gene Names: QIP1; Qip1
• UniProt Entry Name: IMA3_HUMAN

NCBI Description

The nuclear import of karyophilic proteins is directed by short amino acid sequences termed nuclear localization signals (NLSs). Karyopherins, or importins, are cytoplasmic proteins that recognize NLSs and dock NLS-containing proteins to the nuclear pore complex. The protein encoded by this gene shares the sequence similarity with Xenopus importin-alpha and Saccharomyces cerevisiae Srp1. This protein is found to interact with the NLSs of DNA helicase Q1 and SV40 T antigen. [provided by RefSeq, Jul 2008]

Uniprot Description

KPNA4: an alpha importin protein that functions in nuclear protein import as an adapter protein for nuclear receptor KPNB1. Binds specifically and directly to substrates containing either a simple or bipartite NLS motif. Docking of the importin/substrate complex to the nuclear pore complex (NPC) is mediated by KPNB1 through binding to nucleoporin FxFG repeats and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to importin-beta and the three components separate and importin-alpha and -beta are re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran from importin. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. Forms a complex with importin beta-1 subunit.

Protein type: Chaperone; Karyopherin; Adaptor/scaffold; Nuclear import

Chromosomal Location of Human Ortholog: 3q25.33

Cellular Component: nucleoplasm; cytosol

Molecular Function: protein binding; protein transporter activity

Biological Process: response to hydrogen peroxide; cytokine and chemokine mediated signaling pathway; NLS-bearing substrate import into nucleus