Rabbit PYK2 Polyclonal Antibody | anti-PYK2 antibody

PYK2 (I574) Polyclonal Antibody

Cat. Num. AAA3001040

• Gene Names: PTK2B; PKB; PTK; CAKB; FAK2; PYK2; CADTK; FADK2; RAFTK
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: PYK2; Polyclonal Antibody; PYK2 (I574) Polyclonal Antibody; Cell adhesion kinase beta; CAK beta; Calcium-dependent tyrosine kinase; CADTK; Related adhesion focal tyrosine kinase; RAFTK; FAK2; anti-PYK2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: PYK2 (I574) polyclonal antibody detects endogenous levels of PYK2 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 1009

• Applicable Applications for anti-PYK2 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: WB: 1:500-1:1000; IHC: 1:50-1:200
• Immunogen: Synthetic peptide, corresponding to amino acids 360-418 of Human TFDP1.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of PYK2 (I574) pAb at 1:500 dilutionLane1:K562 whole cell lysate (40ug) Lane2:A549 whole cell lysate (40ug) Lane3:Hela whole cell lysate (40ug) Lane4:The Brain tissue lysate of Mouse (40ug) Lane5:The Brain tissue lysate of Rat (40ug))

Immunohistochemistry (IHC)

(Immunohistochemistry (IHC) analyzes of PYK2 (I574) pAb in paraffin-embedded human breast carcinoma tissue at 1:50.)

Related Product Information for anti-PYK2 antibody

Pyk2 is a protein tyrosine kinase related to Focal Adhesion Kinase (p125 FAK). Pyk2 is highly expressed in the central nervous system. It is phosphorylated on tyrosine residues in response to various stimuli that elevate intracellular calcium concentration, as well as by protein kinase C activation.

NCBI and Uniprot Product Information

• NCBI GI #: 27886584
• NCBI GeneID: 2185
• NCBI Accession #: NP_004094.3
• NCBI GenBank Nucleotide #: NP_004094.3
• UniProt Accession #: Q14289
• Molecular Weight: ~ 115kDa
• NCBI Official Full Name: protein-tyrosine kinase 2-beta isoform a
• NCBI Official Synonym Full Names: protein tyrosine kinase 2 beta
• NCBI Official Symbol: PTK2B
• NCBI Official Synonym Symbols: PKB; PTK; CAKB; FAK2; PYK2; CADTK; FADK2; RAFTK
• NCBI Protein Information: protein-tyrosine kinase 2-beta
• UniProt Protein Name: Protein-tyrosine kinase 2-beta
• Protein Family: Pyruvate kinase
• UniProt Gene Name: PTK2B
• UniProt Synonym Gene Names: FAK2; PYK2; RAFTK; CADTK; CAK-beta; CAKB; FADK 2; RAFTK
• UniProt Entry Name: FAK2_HUMAN

NCBI Description

This gene encodes a cytoplasmic protein tyrosine kinase which is involved in calcium-induced regulation of ion channels and activation of the map kinase signaling pathway. The encoded protein may represent an important signaling intermediate between neuropeptide-activated receptors or neurotransmitters that increase calcium flux and the downstream signals that regulate neuronal activity. The encoded protein undergoes rapid tyrosine phosphorylation and activation in response to increases in the intracellular calcium concentration, nicotinic acetylcholine receptor activation, membrane depolarization, or protein kinase C activation. This protein has been shown to bind CRK-associated substrate, nephrocystin, GTPase regulator associated with FAK, and the SH2 domain of GRB2. The encoded protein is a member of the FAK subfamily of protein tyrosine kinases but lacks significant sequence similarity to kinases from other subfamilies. Four transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

Pyk2: a nonreceptor tyrosine kinase of the Fak family. Predominantly expressed in the cells derived from hematopoietic lineages and in the central nervous system. Pyk2 is one of the signaling mediators for G-protein-coupled receptors. Involved in calcium induced regulation of ion channel and activation of the map kinase signaling pathway. Interacts with the SH2 domain of Grb2. May phosphorylate the voltage-gated potassium channel protein Kv1.2. Its activation is highly correlated with the stimulation of c-Jun N-terminal kinase activity. It plays an important role in cell motility such as spreading and migration. Two alternatively spliced isoforms have been described.

Protein type: EC 2.7.10.2; Kinase, protein; Nuclear receptor co-regulator; Protein kinase, tyrosine (non-receptor); Protein kinase, TK; TK group; Fak family

Chromosomal Location of Human Ortholog: 8p21.1

Cellular Component: focal adhesion; dendrite; postsynaptic density; cell cortex; cytosol; lipid raft; N-methyl-D-aspartate selective glutamate receptor complex; nucleoplasm; extrinsic to internal side of plasma membrane; growth cone; cytoskeleton; cell soma; lamellipodium; axon; perinuclear region of cytoplasm; cytoplasm; nucleus

Molecular Function: protein binding; signal transducer activity; calmodulin-dependent protein kinase activity; protein-tyrosine kinase activity; N-methyl-D-aspartate selective glutamate receptor activity; 3-phosphoinositide-dependent protein kinase binding; non-membrane spanning protein tyrosine kinase activity; protein complex binding; ATP binding; receptor binding

Biological Process: focal adhesion formation; positive regulation of JNK activity; regulation of nitric oxide biosynthetic process; regulation of cGMP biosynthetic process; protein amino acid phosphorylation; glial cell proliferation; regulation of cell shape; regulation of inositol trisphosphate biosynthetic process; negative regulation of bone mineralization; elevation of cytosolic calcium ion concentration; cell surface receptor linked signal transduction; tumor necrosis factor-mediated signaling pathway; response to glucose stimulus; protein complex assembly; negative regulation of neuron apoptosis; neurite development; bone resorption; response to drug; negative regulation of myeloid cell differentiation; positive regulation of nitric-oxide synthase activity; response to osmotic stress; positive regulation of cell growth; marginal zone B cell differentiation; positive regulation of peptidyl-tyrosine phosphorylation; positive regulation of angiogenesis; response to ethanol; ionotropic glutamate receptor signaling pathway; response to mechanical stimulus; response to calcium ion; negative regulation of apoptosis; activation of JAK protein; response to cAMP; peptidyl-tyrosine phosphorylation; blood vessel endothelial cell migration; positive regulation of translation; apoptosis; response to lithium ion; negative regulation of potassium ion transport; protein amino acid autophosphorylation; response to hormone stimulus; regulation of calcium-mediated signaling; positive regulation of JNK cascade; signal transduction; positive regulation of synaptic transmission, glutamatergic; negative regulation of cell proliferation; positive regulation of cell proliferation; response to stress; angiogenesis; positive regulation of cell-matrix adhesion; regulation of cell adhesion; integrin-mediated signaling pathway; epidermal growth factor receptor signaling pathway; MAPKKK cascade; positive regulation of phosphoinositide 3-kinase activity; signal complex assembly; response to cocaine; oocyte maturation; regulation of release of sequestered calcium ion into cytosol; response to hydrogen peroxide; positive regulation of actin filament polymerization; positive regulation of protein kinase activity; response to hypoxia; stress fiber formation; innate immune response; cellular defense response; sprouting angiogenesis; vascular endothelial growth factor receptor signaling pathway; positive regulation of cell migration