Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Rabbit anti-Human Pygopus Homolog 1 (PYGO1) Polyclonal Antibody | anti-PYGO1 antibody
Polyclonal Antibody to Pygopus Homolog 1 (PYGO1)
Reactivity
Human
Applications
Immunocytochemistry, Immunohistochemistry, ELISA, Western Blot
Purity
Affinity Chromatography
Synonyms
Pygopus Homolog 1 (PYGO1); Polyclonal Antibody; Polyclonal Antibody to Pygopus Homolog 1 (PYGO1); anti-PYGO1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against PYGO1. It has been selected for its ability to recognize PYGO1 in immunohistochemical staining andwestern blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.
Concentration
500ug/mL (varies by lot)
Sequence
Antigen: The target protein is fused with two N-terminal Tags, His-tag and T7-tag and its sequence is listed below.
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- ASNFVPGNNS NFTSPLESNH SFIPPPNTFG QAKAPPPKQD FTQGATKNTN QNSSAHPPHL NMDDTVNQSN IELKNVNRNN AVNQENSRSS STEATNNNPA NGTQNKPRQP RGAADACTTE KSNKSSLHPN RHGHSSSDPV YPCGICTNEV NDDQDAILCE ASCQKWFHRI CTGMTETAYG LLTAEASAVW
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- ASNFVPGNNS NFTSPLESNH SFIPPPNTFG QAKAPPPKQD FTQGATKNTN QNSSAHPPHL NMDDTVNQSN IELKNVNRNN AVNQENSRSS STEATNNNPA NGTQNKPRQP RGAADACTTE KSNKSSLHPN RHGHSSSDPV YPCGICTNEV NDDQDAILCE ASCQKWFHRI CTGMTETAYG LLTAEASAVW
Sequence Length
419
Applicable Applications for anti-PYGO1 antibody
Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB)
Application Notes
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Immunogen
Recombinant PYGO1 (Ala201~Ala419) expressed in E.coli.
Cross Reactivity
Human
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2083611
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Immunohistochemistry (IHC)
(DAB staining on fromalin fixed paraffin-embedded Kidney tissue))
Immunohistochemistry (IHC)
(DAB staining on fromalin fixed paraffin-embedded Kidney tissue))
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
45,318 Da
NCBI Official Full Name
pygopus homolog 1 isoform 2
NCBI Official Synonym Full Names
pygopus family PHD finger 1
NCBI Official Symbol
PYGO1
NCBI Protein Information
pygopus homolog 1
UniProt Protein Name
Pygopus homolog 1
Protein Family
UniProt Gene Name
PYGO1
Uniprot Description
Involved in signal transduction through the Wnt pathway.
Research Articles on PYGO1
Similar Products
Product Notes
The PYGO1 pygo1 (Catalog #AAA2012997) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Pygopus Homolog 1 (PYGO1) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Pygopus Homolog 1 (PYGO1) can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB). Western blotting: 1:100-400 Immunocytochemistry in formalin fixed cells: 1:100-500 Immunohistochemistry in formalin fixed frozen section: 1:100-500 Immunohistochemistry in paraffin section: 1:50-200 Enzyme-linked Immunosorbent Assay: 1:100-200. Researchers should empirically determine the suitability of the PYGO1 pygo1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Antigen: The target protein is fused with two N-terminal Tags, His-tag and T7-tag and its sequence is listed below. MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- ASNFVPGNNS NFTSPLESNH SFIPPPNTFG QAKAPPPKQD FTQGATKNTN QNSSAHPPHL NMDDTVNQSN IELKNVNRNN AVNQENSRSS STEATNNNPA NGTQNKPRQP RGAADACTTE KSNKSSLHPN RHGHSSSDPV YPCGICTNEV NDDQDAILCE ASCQKWFHRI CTGMTETAYG LLTAEASAVW. It is sometimes possible for the material contained within the vial of "Pygopus Homolog 1 (PYGO1), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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