Rabbit anti-Human PVR Polyclonal Antibody | anti-PVR antibody

PVR, CT (PVR, PVS, Poliovirus receptor, Nectin-like protein 5, CD155) (APC)

Cat. Num. AAA6339229

• Gene Names: PVR; PVS; HVED; CD155; NECL5; TAGE4; Necl-5
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: PVR; Polyclonal Antibody; CT (PVR; PVS; Poliovirus receptor; Nectin-like protein 5; CD155) (APC); CD155; anti-PVR antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

• Applicable Applications for anti-PVR antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 314-342 in the C-terminal region of human PVR, UniProt Accession #NP_006496.3; P15151.
• Conjugate: APC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in K562 cell line lysates (35ug/lane) usingMBS643220. This demonstrates that MBS643220 detected the PVR protein (arrow).)

Related Product Information for anti-PVR antibody

The protein encoded by this gene is a transmembrane glycoprotein belonging to the immunoglobulin superfamily. The external domain mediates cell attachment to the extracellular matrix molecule vitronectin, while its intracellular domain interacts with the dynein light chain Tctex-1/DYNLT1. The gene is specific to the primate lineage, and serves as a cellular receptor for poliovirus in the first step of poliovirus replication. Multiple transcript variants encoding different isoforms have been found for this gene.

Product Categories/Family for anti-PVR antibody

Antibodies; CD Markers

References

1. Nakai, R., et al. Cancer Sci. 101(5):1326-1330(2010). 2. Jugessur, A., et al. PLoS ONE 5 (7), E11493 (2010). 3. Stanietsky, N., et al. Proc. Natl. Acad. Sci. U.S.A. 106(42):17858-17863(2009). 4. Carlsten, M., et al. J. Immunol. 183(8):4921-4930(2009). 5. Kindberg, E., et al. J. Med. Virol. 81(5):933-936(2009).

NCBI and Uniprot Product Information

• NCBI GI #: 209413726
• NCBI GeneID: 5817
• NCBI Accession #: NP_006496.3
• NCBI GenBank Nucleotide #: NM_006505.3
• UniProt Accession #: P15151; P15152; Q15267; Q15268; Q96BJ1; B4DTS9
• Molecular Weight: 42,864 Da
• NCBI Official Full Name: poliovirus receptor isoform alpha
• NCBI Official Synonym Full Names: poliovirus receptor
• NCBI Official Symbol: PVR
• NCBI Official Synonym Symbols: PVS; HVED; CD155; NECL5; TAGE4; Necl-5
• NCBI Protein Information: poliovirus receptor
• UniProt Protein Name: Poliovirus receptor
• Protein Family: Poliovirus receptor
• UniProt Gene Name: PVR
• UniProt Synonym Gene Names: PVS; NECL-5
• UniProt Entry Name: PVR_HUMAN

NCBI Description

The protein encoded by this gene is a transmembrane glycoprotein belonging to the immunoglobulin superfamily. The external domain mediates cell attachment to the extracellular matrix molecule vitronectin, while its intracellular domain interacts with the dynein light chain Tctex-1/DYNLT1. The gene is specific to the primate lineage, and serves as a cellular receptor for poliovirus in the first step of poliovirus replication. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2008]

Uniprot Description

PVR: Mediates NK cell adhesion and triggers NK cell effector functions. Binds two different NK cell receptors: CD96 and CD226. These interactions accumulates at the cell-cell contact site, leading to the formation of a mature immunological synapse between NK cell and target cell. This may trigger adhesion and secretion of lytic granules and IFN-gamma and activate cytoxicity of activated NK cells. May also promote NK cell-target cell modular exchange, and PVR transfer to the NK cell. This transfer is more important in some tumor cells expressing a lot of PVR, and may trigger fratricide NK cell activation, providing tumors with a mechanism of immunoevasion. Plays a role in mediating tumor cell invasion and migration. Serves as a receptor for poliovirus attachment to target cells. May play a role in axonal transport of poliovirus, by targeting virion-PVR-containing endocytic vesicles to the microtubular network through interaction with DYNLT1. This interaction would drive the virus-containing vesicle to the axonal retrograde transport. Belongs to the nectin family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Receptor, misc.; Immunoglobulin superfamily; Membrane protein, integral; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 19q13.2

Cellular Component: extracellular space; cell surface; focal adhesion; cytoplasm; plasma membrane; integral to membrane

Molecular Function: viral receptor activity; protein binding; receptor activity; cell adhesion molecule binding

Biological Process: intercellular junction assembly and maintenance; regulation of immune response; cell migration; entry of virus into host cell; cell-cell adhesion; positive regulation of natural killer cell mediated cytotoxicity directed against tumor cell target; response to virus; susceptibility to natural killer cell mediated cytotoxicity; positive regulation of natural killer cell mediated cytotoxicity