Immunohistochemistry (IHC)
(PURA Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of PURA Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Rabbit PURA Polyclonal Antibody | anti-PURA antibody
PURA Antibody (C-term)
WB~~1:1000
Immunohistochemistry (IHC)
(PURA Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of PURA Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(PURA Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of PURA Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Western Blot (WB)
(PURA Antibody (C-term) western blot analysis in A549,NCI-H292 cell line lysates (35ug/lane).This demonstrates the PURA antibody detected the PURA protein (arrow).)
Western Blot (WB)
(PURA Antibody (C-term) western blot analysis in A549,NCI-H292 cell line lysates (35ug/lane).This demonstrates the PURA antibody detected the PURA protein (arrow).)
DNA-binding protein. It binds preferentially to the single strand
of the purine-rich element termed PUR, which is present at origins
of replication and in gene flanking regions in a variety of
eukaryotes from yeasts through humans. Thus, it is implicated in
the control of both DNA replication and transcription. Deletion of
this gene has been associated with myelodysplastic syndrome and
acute myelogenous leukemia.
Martins-de-Souza, D., et al. Eur Arch Psychiatry Clin Neurosci 259(3):151-163(2009)
Kaminski, R., et al. Cancer Biol. Ther. 7(12):1926-1935(2008)
Darbinian, N., et al. J. Alzheimers Dis. 15(1):71-82(2008)
Wang, L.G., et al. Cancer Res. 68(8):2678-2688(2008)
NCBI and Uniprot Product Information
NCBI Description
This gene product is a sequence-specific, single-stranded DNA-binding protein. It binds preferentially to the single strand of the purine-rich element termed PUR, which is present at origins of replication and in gene flanking regions in a variety of eukaryotes from yeasts through humans. Thus, it is implicated in the control of both DNA replication and transcription. Deletion of this gene has been associated with myelodysplastic syndrome and acute myelogenous leukemia. [provided by RefSeq, Jul 2008]
Uniprot Description
PURA: This is a probable transcription activator that specifically binds the purine-rich single strand of the PUR element located upstream of the MYC gene. May play a role in the initiation of DNA replication and in recombination. Belongs to the PUR DNA-binding protein family.
Protein type: RNA-binding; DNA-binding
Chromosomal Location of Human Ortholog: 5q31
Cellular Component: DNA replication factor A complex; nuclear chromosome, telomeric region; cell soma; dendrite; cytoplasm; nucleus
Molecular Function: RNA polymerase II transcription factor activity, enhancer binding; protein binding; translation repressor activity, nucleic acid binding; double-stranded telomeric DNA binding; SMAD binding; transcription factor binding; purine-rich negative regulatory element binding; single-stranded DNA binding
Biological Process: DNA unwinding during replication; regulation of transcription from RNA polymerase II promoter; transcription from RNA polymerase II promoter; nervous system development; DNA replication initiation; negative regulation of translation; positive regulation of cell proliferation; negative regulation of transcription, DNA-dependent
Disease: Mental Retardation, Autosomal Dominant 31
Research Articles on PURA
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Product Notes
The PURA pura (Catalog #AAA9210593) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 259-287. The PURA Antibody (C-term) reacts with Human (Predicted Reactivity: Mouse) and may cross-react with other species as described in the data sheet. AAA Biotech's PURA can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Immunohistochemistry (IHC), Western Blot (WB). IHC~~1:50~100 WB~~1:1000. Researchers should empirically determine the suitability of the PURA pura for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PURA, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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