Loading...

Skip to main content

Call us on + 1 (800) 604-9114 for more information about our products

Looking for specific datasheet Manual/COA/MSDS?
Request a Manual/COA/MSDS

Interested to get a quote about our products?
Request a Quote

Rabbit anti-Mouse, human PUMA Polyclonal Antibody | anti-PUMA antibody

PUMA (I17) Antibody

Gene Names
Bbc3; Puma
Reactivity
Mouse, human
Applications
Western Blot
Synonyms
PUMA; Polyclonal Antibody; PUMA (I17) Antibody; anti-PUMA antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Mouse, human
Clonality
Polyclonal
Specificity
Mouse and human PUMA alpha and beta forms
Form/Format
200 ug/ml rabbit polyclonal IgG in 1 ml PBS containing 0.1 % sodium azide and 0.2% gelatin.
Concentration
0.200 mg/ml (varies by lot)
Sequence Length
193
Applicable Applications for anti-PUMA antibody
Western Blot (WB)
Application Notes
Western blotting starting dilution 1:200.
Origin
PUMA (I17) is provided as an affinity purified rabbit polyclonal antibody, raised against a peptide mapping to an internal domain of human PUMA.
Immunogen
Synthetic peptide mapping to an internal domain of human PUMA.
Preparation and Storage
Store this product at 4 degree C, do not freeze. The product is stable for one year from the date of shipment.
Related Product Information for anti-PUMA antibody
The Bcl-2 family proteins can function to either suppress or promote cell death and are characterized by the presence of up to four conserved amino acid motifs, termed Bcl-2 homology (BH) domains (1). The BH3 function has been further revealed by an emerging group of BH3-only proteins, that includes Bik, Bad, Bid, Bim, Noxa, and the newest member PUMA or bbc3 (1&2). The PUMA gene is a target of p53 transcriptional activation and encodes two BH3 domain-containing proteins (PUMA-alpha and PUMA-beta) (3&4). The activities of PUMA-alpha and PUMA-beta are similar; they bind to Bcl-2, localize to the mitochondria to induce cytochrome c release, and activate extremely rapid apoptosis (3&4).
References
1.) Goldberg Z, Levav Y, Krichevsky S, Fibach E, Haupt Y. 2004. Treatment of chronic myeloid leukemia cells with imatinib (STI571) impairs p53 accumulation in response to DNA damage. Cell Cycle. Sep;3(9):1188-95.

1. Han J., Flemington C., Houghton A.B., Gu Z., Zambetti G.P., Lutz R.J., Zhu L., Chittenden T. 2001. Expression of bbc3, a pro-apoptotic BH3-only gene, is regulated by diverse cell death and survival signals. PNAS 98(20):11318-11323.2. Schuler M., Green D.R. 2001. Mechanisms of p53-dependent apoptosis. Biochem Soc Trans 29(Pt6):684-688.3. Nakano K., Vousden K.H. 2001. PUMA, a novel proapoptotic gene, is induced by p53. Mol Cell 7(3):683-694.4. Yu J., Zhang L., Hwang P.M., Kinzler K.W., Vogelstein B. 2001. PUMA induces the rapid apoptosis of colorectal cancer cells. Mol Cell 7(3):673-682.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
20,718 Da
NCBI Official Full Name
PUMA
NCBI Official Synonym Full Names
Bcl-2 binding component 3
NCBI Official Symbol
Bbc3
NCBI Official Synonym Symbols
Puma
NCBI Protein Information
bcl-2-binding component 3; p53 up-regulated modulator of apoptosis
UniProt Protein Name
Bcl-2-binding component 3
UniProt Gene Name
Bbc3
UniProt Synonym Gene Names
Puma
UniProt Entry Name
BBC3_RAT

Uniprot Description

PUMA: Essential mediator of p53/TP53-dependent and p53/TP53- independent apoptosis. Interacts with MCL1 and BCL2A1. Interacts with BCL2 and BCL2L1/BCL-XL. By DNA damage, glucocorticoid treatment, growth factor deprivation and p53/TP53. Ubiquitously expressed. Belongs to the Bcl-2 family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Mitochondrial

Cellular Component: mitochondrion; intracellular

Molecular Function: protein binding

Biological Process: caspase activation; release of cytochrome c from mitochondria; glial cell differentiation; positive regulation of protein homooligomerization; positive regulation of apoptosis; release of sequestered calcium ion into cytosol; reduction of endoplasmic reticulum calcium ion concentration; positive regulation of neuron apoptosis; brain development; determination of adult life span; DNA damage response, signal transduction by p53 class mediator resulting in induction of apoptosis; response to DNA damage stimulus; negative regulation of growth

Research Articles on PUMA

Similar Products

Product Notes

The PUMA bbc3 (Catalog #AAA440040) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PUMA (I17) Antibody reacts with Mouse, human and may cross-react with other species as described in the data sheet. AAA Biotech's PUMA can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Western blotting starting dilution 1:200. Researchers should empirically determine the suitability of the PUMA bbc3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PUMA, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

Item has been added to Shopping Cart

If you are ready to order, navigate to Shopping Cart and get ready to checkout.

Looking for a specific manual?
Request a Manual

Request more Information

Please complete the form below and a representative will contact you as soon as possible.

Request a Manual

Please complete the form below and a representative will contact you as soon as possible.

Request a Quote

Please complete the form below and a representative will contact you as soon as possible.