Western Blot (WB)
(Fig-1: Western blot analysis of PU.1. Anti-PU.1 antibody (11-4041) was used at 4 ug/ml on Spleen lysate.)
Rabbit anti-Human PU.1 Polyclonal Antibody | anti-PU.1 antibody
Polyclonal Antibody to PU.1
Protein A Chromatography
Protein A Chromatography
For long-term storage, store at -20 degree C.
Avoid repeated freeze and thaw cycles.
Western Blot (WB)
(Fig-1: Western blot analysis of PU.1. Anti-PU.1 antibody (11-4041) was used at 4 ug/ml on Spleen lysate.)
Western Blot (WB)
(Fig-1: Western blot analysis of PU.1. Anti-PU.1 antibody (11-4041) was used at 4 ug/ml on Spleen lysate.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes an ETS-domain transcription factor that activates gene expression during myeloid and B-lymphoid cell development. The nuclear protein binds to a purine-rich sequence known as the PU-box found near the promoters of target genes, and regulates their expression in coordination with other transcription factors and cofactors. The protein can also regulate alternative splicing of target genes. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Uniprot Description
PU.1: Binds to the PU-box, a purine-rich DNA sequence (5'- GAGGAA-3') that can act as a lymphoid-specific enhancer. This protein is a transcriptional activator that may be specifically involved in the differentiation or activation of macrophages or B- cells. Also binds RNA and may modulate pre-mRNA splicing. Binds DNA as a monomer. Interacts with CEBPD and NONO. Interacts with RUNX1 and SPIB. Interacts with GFI1; the interaction represses SPI1 transcriptional activity. Highly expressed in both FV-P and FV-A-induced erythro- leukemia cell lines that have undergone rearrangements of the Spi- 1 gene due to the insertion of SFFV. Belongs to the ETS family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: DNA-binding; Oncoprotein; Transcription factor
Chromosomal Location of Human Ortholog: 11p11.2
Cellular Component: nuclear chromatin
Molecular Function: NFAT protein binding; protein binding; RNA polymerase II transcription factor activity, enhancer binding; transcription factor activity
Biological Process: negative regulation of gene expression, epigenetic; negative regulation of transcription, DNA-dependent; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; regulation of erythrocyte differentiation
Research Articles on PU.1
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Product Notes
The PU.1 spi1 (Catalog #AAA668761) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to PU.1 reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PU.1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 2-4 ug/ml. Researchers should empirically determine the suitability of the PU.1 spi1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PU.1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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