Rabbit anti-Human PTRF Polyclonal Antibody | anti-PTRF antibody

PTRF, CT (Polymerase I and Transcript Release Factor, Cavin-1, FKSG13) (MaxLight 650)

Cat. Num. AAA6496053

• Gene Names: PTRF; CGL4; CAVIN; CAVIN1; FKSG13; cavin-1
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Ammonium Sulfate Precipitation.
• Synonyms: PTRF; Polyclonal Antibody; CT (Polymerase I and Transcript Release Factor; Cavin-1; FKSG13) (MaxLight 650); anti-PTRF antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human PTRF.
• Purity/Purification: Purified by Ammonium Sulfate Precipitation.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight650.

• Applicable Applications for anti-PTRF antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:50-1:100; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the C-terminal region of human PTRF (KLH).
• Conjugate: MaxLight650
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of anti-PTRF Antibody (N-term) Pab in A375 cell line lysates (35ug/lane). PTRF(arrow) was detected using the purified Pab.)

Product Categories/Family for anti-PTRF antibody

Antibodies; Transcription Factors

References

1. Aboulaich, N., Biochem. Biophys. Res. Commun. 350(3), 657-661 (2006). 2. Aboulaich, N., Biochem. J. 383(PT 2), 237-248 (2004). 3. Hasegawa, T., Biochem. J. 347 PT 1, 55-59 (2000).

NCBI and Uniprot Product Information

• NCBI GI #: 42734430
• NCBI GeneID: 284119
• NCBI Accession #: NP_036364
• NCBI GenBank Nucleotide #: NM_012232.5
• UniProt Accession #: Q6NZI2; O00535; Q6GMY1; Q96H74; Q9BT85; Q9HAP4; B2RAW7
• Molecular Weight: 43 kDa
• NCBI Official Full Name: polymerase I and transcript release factor
• NCBI Official Synonym Full Names: polymerase I and transcript release factor
• NCBI Official Symbol: PTRF
• NCBI Official Synonym Symbols: CGL4; CAVIN; CAVIN1; FKSG13; cavin-1
• NCBI Protein Information: polymerase I and transcript release factor; PTRF; TTF-I interacting peptide 12; RNA polymerase I and transcript release factor
• UniProt Protein Name: Polymerase I and transcript release factor
• Protein Family: Polymerase I and transcript release factor
• UniProt Gene Name: PTRF
• UniProt Entry Name: PTRF_HUMAN

NCBI Description

This gene encodes a protein that enables the dissociation of paused ternary polymerase I transcription complexes from the 3' end of pre-rRNA transcripts. This protein regulates rRNA transcription by promoting the dissociation of transcription complexes and the reinitiation of polymerase I on nascent rRNA transcripts. This protein also localizes to caveolae at the plasma membrane and is thought to play a critical role in the formation of caveolae and the stabilization of caveolins. This protein translocates from caveolae to the cytoplasm after insulin stimulation. Caveolae contain truncated forms of this protein and may be the site of phosphorylation-dependent proteolysis. This protein is also thought to modify lipid metabolism and insulin-regulated gene expression. Mutations in this gene result in a disorder characterized by generalized lipodystrophy and muscular dystrophy. [provided by RefSeq, Nov 2009]

Uniprot Description

PTRF: Plays an important role in caveolae formation and organization. Required for the sequestration of mobile caveolin into immobile caveolae. Termination of transcription by RNA polymerase I involves pausing of transcription by TTF1, and the dissociation of the transcription complex, releasing pre-rRNA and RNA polymerase I from the template. PTRF is required for dissociation of the ternary transcription complex. Defects in PTRF are the cause of congenital generalized lipodystrophy type 4 (CGL4). It is a disorder characterized by the association of congenital generalized lipodystrophy with muscular dystrophy and cardiac anomalies. Congenital generalized lipodystrophy is characterized by a near complete absence of adipose tissue, extreme insulin resistance, hypertriglyceridemia, hepatic steatosis and early onset of diabetes. Belongs to the PTRF/SDPR family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Transcription initiation complex

Chromosomal Location of Human Ortholog: 17q21.2

Cellular Component: nucleoplasm; protein complex; mitochondrion; intracellular membrane-bound organelle; endoplasmic reticulum; cytoplasm; plasma membrane; caveola; cytosol; nucleus

Molecular Function: protein binding; rRNA primary transcript binding

Biological Process: regulation of transcription, DNA-dependent; transcription from RNA polymerase I promoter; gene expression; termination of RNA polymerase I transcription; transcription initiation from RNA polymerase I promoter

Disease: Lipodystrophy, Congenital Generalized, Type 4