Western Blot (WB)
(Western blot analysis of extracts of C2C12 and PC-12 cells, using Phospho-PTPRA-Y789 antibody at 1:1000 dilution. C2C12 cell lysate were treated by CIP (20ul CIP for each 400ul cell lysate) at 37 degree C for 1 hour.PC-12 cell lysate were treated by CIP (20ul CIP for each 400ul cell lysate) at 37 degree C for 1 hour.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit.Exposure time: 30s.)
Rabbit PTPRA-Y789 Polyclonal Antibody | anti-PTPRA-Y789 antibody
Phospho-PTPRA-Y789 pAb
Gene Names
PTPRA; LRP; HLPR; PTPA; HEPTP; HPTPA; RPTPA; PTPRL2; HPTPalpha; R-PTP-alpha
Reactivity
Human, Mouse, Rat
Applications
Western Blot
Purity
Affinity Purification
Synonyms
PTPRA-Y789; Polyclonal Antibody; Phospho-PTPRA-Y789 pAb; HEPTP; HLPR; HPTPA; HPTPalpha; LRP; PTPA; PTPRL2; R-PTP-alpha; RPTPA; anti-PTPRA-Y789 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity Purification
Sequence Length
802
Applicable Applications for anti-PTPRA-Y789 antibody
Western Blot (WB)
Application Notes
WB: 1:500 - 1:2000
Immunogen
A phospho specific peptide corresponding to residues surrounding Y789 of human PTPRA
Immunogen Species
Human
Storage Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Cellular Location
Membrane, Single-pass type I membrane protein
Positive Samples
C2C12, PC-12
Preparation and Storage
Store at -20 degree C. Avoid freeze / thaw cycles.
Western Blot (WB)
(Western blot analysis of extracts of C2C12 and PC-12 cells, using Phospho-PTPRA-Y789 antibody at 1:1000 dilution. C2C12 cell lysate were treated by CIP (20ul CIP for each 400ul cell lysate) at 37 degree C for 1 hour.PC-12 cell lysate were treated by CIP (20ul CIP for each 400ul cell lysate) at 37 degree C for 1 hour.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit.Exposure time: 30s.)
Western Blot (WB)
(Western blot analysis of extracts of C2C12 and PC-12 cells, using Phospho-PTPRA-Y789 antibody at 1:1000 dilution. C2C12 cell lysate were treated by CIP (20ul CIP for each 400ul cell lysate) at 37 degree C for 1 hour.PC-12 cell lysate were treated by CIP (20ul CIP for each 400ul cell lysate) at 37 degree C for 1 hour.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit.Exposure time: 30s.)
Related Product Information for anti-PTPRA-Y789 antibody
The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP contains an extracellular domain, a single transmembrane segment and two tandem intracytoplasmic catalytic domains, and thus represents a receptor-type PTP. This PTP has been shown to dephosphorylate and activate Src family tyrosine kinases, and is implicated in the regulation of integrin signaling, cell adhesion and proliferation. Three alternatively spliced variants of this gene, which encode two distinct isoforms, have been reported.
Product Categories/Family for anti-PTPRA-Y789 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Calculated: 89kDa; 90kDa
Observed: 145kDa
Observed: 145kDa
NCBI Official Full Name
receptor-type tyrosine-protein phosphatase alpha isoform 1
NCBI Official Synonym Full Names
protein tyrosine phosphatase, receptor type A
NCBI Official Symbol
PTPRA
NCBI Official Synonym Symbols
LRP; HLPR; PTPA; HEPTP; HPTPA; RPTPA; PTPRL2; HPTPalpha; R-PTP-alpha
NCBI Protein Information
receptor-type tyrosine-protein phosphatase alpha
UniProt Protein Name
Receptor-type tyrosine-protein phosphatase alpha
UniProt Gene Name
PTPRA
UniProt Synonym Gene Names
PTPA; PTPRL2; Protein-tyrosine phosphatase alpha; R-PTP-alpha
NCBI Description
The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP contains an extracellular domain, a single transmembrane segment and two tandem intracytoplasmic catalytic domains, and thus represents a receptor-type PTP. This PTP has been shown to dephosphorylate and activate Src family tyrosine kinases, and is implicated in the regulation of integrin signaling, cell adhesion and proliferation. Three alternatively spliced variants of this gene, which encode two distinct isoforms, have been reported. [provided by RefSeq, Jul 2008]
Research Articles on PTPRA-Y789
Similar Products
Product Notes
The PTPRA-Y789 ptpra (Catalog #AAA9135667) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Phospho-PTPRA-Y789 pAb reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's PTPRA-Y789 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:500 - 1:2000. Researchers should empirically determine the suitability of the PTPRA-Y789 ptpra for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PTPRA-Y789, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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