Rabbit anti-Human PTPN5 Polyclonal Antibody | anti-PTPN5 antibody

PTPN5, NT (PTPN5, Tyrosine-protein phosphatase non-receptor type 5, Neural-specific protein-tyrosine phosphatase, Striatum-enriched protein-tyrosine phosphatase) (APC)

Cat. Num. AAA6339020

• Gene Names: PTPN5; STEP; PTPSTEP
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: PTPN5; Polyclonal Antibody; NT (PTPN5; Tyrosine-protein phosphatase non-receptor type 5; Neural-specific protein-tyrosine phosphatase; Striatum-enriched protein-tyrosine phosphatase) (APC); Striatum-enriched protein-tyrosine phosphatase; anti-PTPN5 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

• Applicable Applications for anti-PTPN5 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: PTPN5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human PTPN5.
• Conjugate: APC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(PTPN5 Antibody (N-term) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the PTPN5 antibody detected the PTPN5 protein (arrow).)

Related Product Information for anti-PTPN5 antibody

STEP (Protein-tyrosine phosphatase striatum-enriched) is a neural-specific protein-tyrosine phosphatase. It is also known as PTPN5 (tyrosine-protein phosphatase, non-receptor type 5). STEP immunoreactive areas are most intense in the regions of the CNS receiving a dopaminergic input (e.g. striatum, basal ganglia). STEP is part of the NMDA receptor (R) complex and inhibits NMDAR single-channel activity and NMDAR-mediated synaptic current. Phosphorylation of STEP, via D1 and PKA activation by dopamine, decreases the activity of STEP by reducing its affinity for substrate.

Product Categories/Family for anti-PTPN5 antibody

Antibodies; Enzymes; Phosphatase

References

Kurup, P., et al. J. Neurosci. 30(17):5948-5957(2010). Rose, J. Phd, et al. Mol. Med. (2010). In press: Korff, S., et al. BMC Cancer 8, 329 (2008): Eswaran, J., et al. Biochem. J. 395(3):483-491(2006). Nguyen, T.H., et al. J. Biol. Chem. 277(27):24274-24279(2002).

NCBI and Uniprot Product Information

• NCBI GI #: 90652861
• NCBI GeneID: 84867
• NCBI Accession #: NP_001035059.1; NP_008837.1
• NCBI GenBank Nucleotide #: NM_001039970.1
• UniProt Accession #: P54829; Q6P1Z2; Q8N2A1; Q8NDP8; B3KXG7; B7Z386; B7ZAF5; D3DQY7
• Molecular Weight: 60,961 Da
• NCBI Official Full Name: tyrosine-protein phosphatase non-receptor type 5 isoform b
• NCBI Official Synonym Full Names: protein tyrosine phosphatase, non-receptor type 5 (striatum-enriched)
• NCBI Official Symbol: PTPN5
• NCBI Official Synonym Symbols: STEP; PTPSTEP
• NCBI Protein Information: tyrosine-protein phosphatase non-receptor type 5; neural-specific protein-tyrosine phosphatase; protein-tyrosine phosphatase striatum-enriched; striatum-enriched protein-tyrosine phosphatase
• UniProt Protein Name: Tyrosine-protein phosphatase non-receptor type 5
• Protein Family: Tyrosine-protein phosphatase non-receptor
• UniProt Gene Name: PTPN5
• UniProt Synonym Gene Names: STEP
• UniProt Entry Name: PTN5_HUMAN

Uniprot Description

STEP: a non-receptor protein-tyrosine phosphatase. STEP (striatum enriched phosphatase) Contains a single phosphatase catalytic domain located at the C-terminus. Two alternatively-spliced messages have been described.

Protein type: Motility/polarity/chemotaxis; Protein phosphatase, tyrosine (non-receptor); EC 3.1.3.48; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 11p15.1

Cellular Component: endoplasmic reticulum membrane; integral to membrane

Molecular Function: phosphotyrosine binding; protein tyrosine phosphatase activity

Biological Process: protein amino acid dephosphorylation