Rabbit anti-Human PTPN2 Polyclonal Antibody | anti-PTPN2 antibody

PTPN2, CT (PTPN2, PTPT, Tyrosine-protein phosphatase non-receptor type 2, T-cell protein-tyrosine phosphatase) (FITC)

Cat. Num. AAA6338978

• Gene Names: PTPN2; PTN2; PTPT; TCPTP; TC-PTP; TCELLPTP
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: PTPN2; Polyclonal Antibody; CT (PTPN2; PTPT; Tyrosine-protein phosphatase non-receptor type 2; T-cell protein-tyrosine phosphatase) (FITC); T-cell protein-tyrosine phosphatase; anti-PTPN2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).
• Sequence Length: 387

• Applicable Applications for anti-PTPN2 antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:10-50; FLISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: PTPN2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 328-358 amino acids from the C-terminal region of human PTPN2.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(PTPN2 Antibody (C-term) western blot analysis in CEM cell line lysates (35ug/lane).This demonstrates the PTPN2 antibody detected the PTPN2 protein (arrow).)

Immunohistochemistry (IHC)

(PTPN2 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human placenta tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of PTPN2 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Related Product Information for anti-PTPN2 antibody

The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. Members of the PTP family share a highly conserved catalytic motif, which is essential for the catalytic activity. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. Epidermal growth factor receptor and the adaptor protein Shc were reported to be substrates of this PTP, which suggested the roles in growth factor mediated cell signaling. Three alternatively spliced variants of this gene, which encode isoforms differing at their extreme C-termini, have been described. The different C-termini are thought to determine the substrate specificity, as well as the cellular localization of the isoforms. Two highly related but distinctly processed pseudogenes that localize to distinct chromosomes have been reported. [provided by RefSeq].

Product Categories/Family for anti-PTPN2 antibody

Antibodies; Enzymes; Phosphatase

References

Scharl, M., et al. Am. J. Physiol. Gastrointest. Liver Physiol. 299 (4), G935-G945 (2010): Morgan, A.R., et al. Tissue Antigens 76(2):119-125(2010). Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010): Kleppe, M., et al. Nat. Genet. 42(6):530-535(2010). Amre, D.K., et al. Aliment. Pharmacol. Ther. 31(11):1186-1191(2010).

NCBI and Uniprot Product Information

• NCBI GI #: 18104980
• NCBI GeneID: 5771
• NCBI Accession #: NP_536347.1
• NCBI GenBank Nucleotide #: NP_536347.1
• UniProt Accession #: P17706
• NCBI Official Full Name: tyrosine-protein phosphatase non-receptor type 2 isoform 2
• NCBI Official Synonym Full Names: protein tyrosine phosphatase non-receptor type 2
• NCBI Official Symbol: PTPN2
• NCBI Official Synonym Symbols: PTN2; PTPT; TCPTP; TC-PTP; TCELLPTP
• NCBI Protein Information: tyrosine-protein phosphatase non-receptor type 2
• UniProt Protein Name: Tyrosine-protein phosphatase non-receptor type 2
• Protein Family: Tyrosine-protein phosphatase non-receptor
• UniProt Gene Name: PTPN2
• UniProt Synonym Gene Names: PTPT; TCPTP
• UniProt Entry Name: PTN2_HUMAN

NCBI Description

The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. Members of the PTP family share a highly conserved catalytic motif, which is essential for the catalytic activity. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. Epidermal growth factor receptor and the adaptor protein Shc were reported to be substrates of this PTP, which suggested the roles in growth factor mediated cell signaling. Multiple alternatively spliced transcript variants encoding different isoforms have been found. Two highly related but distinctly processed pseudogenes that localize to chromosomes 1 and 13, respectively, have been reported. [provided by RefSeq, May 2011]

Uniprot Description

PTPN2: a type 2 non-receptor phospho-tyrosine-protein phosphatase (PTP). Two alternatively spliced isoforms are differenyially expressed. Isoform A is a major PTP expressed in human tissues, while isoform B is expressed mainly in T-cells and in placenta.

Protein type: Protein phosphatase, tyrosine (non-receptor); EC 3.1.3.48; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 18p11.3-p11.2

Cellular Component: nucleoplasm; endoplasmic reticulum; ER-Golgi intermediate compartment; plasma membrane; nucleus

Molecular Function: integrin binding; protein binding; syntaxin binding; protein tyrosine phosphatase activity; receptor tyrosine kinase binding; protein kinase binding

Biological Process: negative regulation of epidermal growth factor receptor signaling pathway; negative regulation of tyrosine phosphorylation of Stat1 protein; cytokine and chemokine mediated signaling pathway; negative regulation of insulin receptor signaling pathway; negative regulation of T cell receptor signaling pathway; negative regulation of tyrosine phosphorylation of Stat5 protein; negative regulation of chemotaxis; glucose homeostasis; negative regulation of cell proliferation; positive regulation of gluconeogenesis; negative regulation of macrophage differentiation; negative regulation of inflammatory response; B cell differentiation; negative regulation of tyrosine phosphorylation of Stat3 protein; insulin receptor signaling pathway; negative regulation of tyrosine phosphorylation of Stat6 protein; erythrocyte differentiation; T cell differentiation