Rabbit anti-Human, Mouse PTP4A1 Polyclonal Antibody | anti-PTP4A1 antibody

PTP4A1, CT (PTP4A1, PRL1, PTPCAAX1, Protein tyrosine phosphatase type IVA 1, PTP(CAAXI), Protein-tyrosine phosphatase 4a1, Protein-tyrosine phosphatase of regenerating liver 1) (FITC)

Cat. Num. AAA6338901

• Gene Names: PTP4A1; HH72; PRL1; PRL-1; PTPCAAX1; PTP(CAAX1)
• Reactivity: Human, Mouse
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: PTP4A1; Polyclonal Antibody; CT (PTP4A1; PRL1; PTPCAAX1; Protein tyrosine phosphatase type IVA 1; PTP(CAAXI); Protein-tyrosine phosphatase 4a1; Protein-tyrosine phosphatase of regenerating liver 1) (FITC); Protein-tyrosine phosphatase of regenerating liver 1; anti-PTP4A1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-PTP4A1 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: PTP4A1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 139-168 amino acids from the C-terminal region of human PTP4A1.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(PTP4A1 Antibody (C-term) western blot analysis in mouse bladder tissue lysates (35ug/lane).This demonstrates the PTP4A1 antibody detected the PTP4A1 protein (arrow).)

Western Blot (WB)

(Western blot analysis of PTP4A1 (arrow) using rabbit polyclonal PTP4A1 Antibody (C-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the PTP4A1 gene.)

Related Product Information for anti-PTP4A1 antibody

The protein encoded by this gene belongs to a small class of prenylated protein tyrosine phosphatases (PTPs), which contains a PTP domain and a characteristic C-terminal prenylation motif. PTPs are cell signaling molecules that play regulatory roles in a variety of cellular processes. This tyrosine phosphatase is a nuclear protein, but may primarily associate with plasma membrane. The surface membrane association of this protein depends on its C-terminal prenylation. Overexpression of this gene in mammalian cells conferred a transformed phenotype, which implicated its role in the tumorigenesis. Studies in rat suggested that this gene may be an immediate-early gene in mitogen-stimulated cells. [provided by RefSeq].

Product Categories/Family for anti-PTP4A1 antibody

Antibodies; Enzymes; Phosphatase

References

Johnatty, S.E., et al. PLoS Genet. 6 (7), E1001016 (2010): Skinner, A.L., et al. Biochemistry 48(20):4262-4272(2009). Luo, Y., et al. Biochemistry 48(8):1838-1846(2009). Min, S.H., et al. Oncogene 28(4):545-554(2009). Liu, Y.Q., et al. Arch. Pathol. Lab. Med. 132(8):1307-1312(2008).

NCBI and Uniprot Product Information

• NCBI GI #: 4506283
• NCBI GeneID: 7803
• NCBI Accession #: NP_003454.1
• NCBI GenBank Nucleotide #: NM_003463.4
• UniProt Accession #: Q93096; O00648; Q49A54; B2R6C8
• Molecular Weight: 19,815 Da
• NCBI Official Full Name: protein tyrosine phosphatase type IVA 1
• NCBI Official Synonym Full Names: protein tyrosine phosphatase type IVA, member 1
• NCBI Official Symbol: PTP4A1
• NCBI Official Synonym Symbols: HH72; PRL1; PRL-1; PTPCAAX1; PTP(CAAX1)
• NCBI Protein Information: protein tyrosine phosphatase type IVA 1; protein tyrosine phosphatase type IVA protein 1; protein-tyrosine phosphatase 4a1; protein-tyrosine phosphatase of regenerating liver 1
• UniProt Protein Name: Protein tyrosine phosphatase type IVA 1
• Protein Family: Protein tyrosine phosphatase
• UniProt Gene Name: PTP4A1
• UniProt Synonym Gene Names: PRL1; PTPCAAX1; PRL-1
• UniProt Entry Name: TP4A1_HUMAN

NCBI Description

This gene encodes a member of a small class of prenylated protein tyrosine phosphatases (PTPs), which contain a PTP domain and a characteristic C-terminal prenylation motif. The encoded protein is a cell signaling molecule that plays regulatory roles in a variety of cellular processes, including cell proliferation and migration. The protein may also be involved in cancer development and metastasis. This tyrosine phosphatase is a nuclear protein, but may associate with plasma membrane by means of its prenylation motif. Pseudogenes related to this gene are located on chromosomes 1, 2, 5, 7, 11 and X. [provided by RefSeq, Jun 2013]

Uniprot Description

PTP4A1: Protein tyrosine phosphatase which stimulates progression from G1 into S phase during mitosis. May play a role in the development and maintenance of differentiating epithelial tissues. Enhances cell proliferation, cell motility and invasive activity, and promotes cancer metastasis. Belongs to the protein-tyrosine phosphatase family.

Protein type: EC 3.1.3.48; Protein phosphatase, tyrosine (non-receptor); Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 6q12

Cellular Component: internal side of plasma membrane; endoplasmic reticulum; early endosome; cytoplasm; spindle

Molecular Function: protein tyrosine phosphatase activity

Biological Process: multicellular organismal development; cell cycle; positive regulation of cell migration