Rabbit anti-Human, Mouse PTOV1 Polyclonal Antibody | anti-PTOV1 antibody

PTOV1, NT (PTOV1, ACID2, Prostate tumor-overexpressed gene 1 protein, Activator interaction domain-containing protein 2) (AP)

Cat. Num. AAA6338887

• Gene Names: PTOV1; ACID2; PTOV-1
• Reactivity: Human, Mouse
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: PTOV1; Polyclonal Antibody; NT (PTOV1; ACID2; Prostate tumor-overexpressed gene 1 protein; Activator interaction domain-containing protein 2) (AP); Activator interaction domain-containing protein 2; anti-PTOV1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).

• Applicable Applications for anti-PTOV1 antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:50-100; ELISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: PTOV1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 28-56 amino acids from the N-terminal region of human PTOV1.
• Conjugate: AP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(PTOV1 Antibody (N-term) western blot analysis in mouse cerebellum tissue lysates (35ug/lane).This demonstrates the PTOV1 antibody detected the PTOV1 protein (arrow).)

Immunohistochemistry (IHC)

(PTOV1 antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the PTOV1 antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Related Product Information for anti-PTOV1 antibody

May activate transcription. Required for nuclear translocation of FLOT1. Promotes cell proliferation.

Product Categories/Family for anti-PTOV1 antibody

Antibodies; Disease Markers

References

Morote, J., et al. Clin. Cancer Res. 14(9):2617-2622(2008). Santamaria, A., et al. Mol. Cell. Biol. 25(5):1900-1911(2005). Mittler, G., et al. EMBO J. 22(24):6494-6504(2003). Clark, H.F., et al. Genome Res. 13(10):2265-2270(2003). Santamaria, A., et al. Am. J. Pathol. 162(3):897-905(2003).

NCBI and Uniprot Product Information

• NCBI GI #: 33695090
• NCBI GeneID: 53635
• NCBI Accession #: NP_059128.2
• NCBI GenBank Nucleotide #: NM_017432.3
• UniProt Accession #: Q86YD1; Q6UXX7; Q96BU3; Q9HBN4; Q9NYL1
• Molecular Weight: 46,869 Da
• NCBI Official Full Name: prostate tumor-overexpressed gene 1 protein
• NCBI Official Synonym Full Names: prostate tumor overexpressed 1
• NCBI Official Symbol: PTOV1
• NCBI Official Synonym Symbols: ACID2; PTOV-1
• NCBI Protein Information: prostate tumor-overexpressed gene 1 protein; prostate tumor overexpressed gene 1; activator interaction domain-containing protein 2
• UniProt Protein Name: Prostate tumor-overexpressed gene 1 protein
• Protein Family: Prostate tumor-overexpressed gene 1 protein
• UniProt Gene Name: PTOV1
• UniProt Synonym Gene Names: ACID2; PTOV-1
• UniProt Entry Name: PTOV1_HUMAN

NCBI Description

This gene encodes a protein that was found to be overexpressed in prostate adenocarcinomas. The encoded protein was found to interact with the lipid raft protein flotillin-1 and shuttle it from the cytoplasm to the nucleus in a cell cycle dependent manner. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Feb 2015]

Uniprot Description

PTOV1: May activate transcription. Required for nuclear translocation of FLOT1. Promotes cell proliferation. Belongs to the Mediator complex subunit 25 family. PTOV1 subfamily. 3 isoforms of the human protein are produced by alternative splicing.

Chromosomal Location of Human Ortholog: 19q13.33

Cellular Component: nucleus; perinuclear region of cytoplasm; plasma membrane

Biological Process: regulation of transcription, DNA-dependent; transcription, DNA-dependent