Rabbit anti-Human PTGR2 Polyclonal Antibody | anti-PTGR2 antibody

PTGR2, NT (PTGR2, ZADH1, Prostaglandin reductase 2, 15-oxoprostaglandin 13-reductase, Zinc-binding alcohol dehydrogenase domain-containing protein 1) (PE)

Cat. Num. AAA6338754

• Gene Names: PTGR2; PGR2; ZADH1
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: PTGR2; Polyclonal Antibody; NT (PTGR2; ZADH1; Prostaglandin reductase 2; 15-oxoprostaglandin 13-reductase; Zinc-binding alcohol dehydrogenase domain-containing protein 1) (PE); Zinc-binding alcohol dehydrogenase domain-containing protein 1; anti-PTGR2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-PTGR2 antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:10-50; FLISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: PTGR2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 72-102 amino acids from the N-terminal region of human PTGR2.
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(PTGR2 Antibody (N-term) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the PTGR2 antibody detected the PTGR2 protein (arrow).)

Immunohistochemistry (IHC)

(PTGR2 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of PTGR2 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Related Product Information for anti-PTGR2 antibody

This gene encodes an enzyme involved in the metabolism of prostaglandins. The encoded protein catalyzes the NADPH-dependent conversion of 15-keto-prostaglandin E2 to 15-keto-13,14-dihydro-prostaglandin E2. This protein may also be involved in regulating activation of the peroxisome proliferator-activated receptor. Alternative splicing results in multiple transcript variants.

Product Categories/Family for anti-PTGR2 antibody

Antibodies; Prostaglandins

References

Wu, Y.H., et al. Structure 16(11):1714-1723(2008). Gao, K., et al. Int. J. Oncol. 32(6):1343-1349(2008). Chou, W.L., et al. J. Biol. Chem. 282(25):18162-18172(2007). Lamesch, P., et al. Genomics 89(3):307-315(2007). Zhang, L., et al. Cytogenet. Genome Res. 103 (1-2), 79-83 (2003).

NCBI and Uniprot Product Information

• NCBI GI #: 226246680
• NCBI GeneID: 145482
• NCBI Accession #: NP_001139626.1
• NCBI GenBank Nucleotide #: NM_001146154.1
• UniProt Accession #: Q8N8N7; Q3L8A4; Q6MZH8
• Molecular Weight: 38,499 Da
• NCBI Official Full Name: prostaglandin reductase 2
• NCBI Official Synonym Full Names: prostaglandin reductase 2
• NCBI Official Symbol: PTGR2
• NCBI Official Synonym Symbols: PGR2; ZADH1
• NCBI Protein Information: prostaglandin reductase 2; PRG-2; 15-oxoprostaglandin 13-reductase; 15-oxoprostaglandin-delta13-reductase; zinc binding alcohol dehydrogenase, domain containing 1; zinc-binding alcohol dehydrogenase domain-containing protein 1
• UniProt Protein Name: Prostaglandin reductase 2
• Protein Family: Prostaglandin reductase
• UniProt Gene Name: PTGR2
• UniProt Synonym Gene Names: ZADH1; PRG-2
• UniProt Entry Name: PTGR2_HUMAN

NCBI Description

This gene encodes an enzyme involved in the metabolism of prostaglandins. The encoded protein catalyzes the NADPH-dependent conversion of 15-keto-prostaglandin E2 to 15-keto-13,14-dihydro-prostaglandin E2. This protein may also be involved in regulating activation of the peroxisome proliferator-activated receptor. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2009]

Uniprot Description

PTGR2: Functions as 15-oxo-prostaglandin 13-reductase and acts on 15-keto-PGE1, 15-keto-PGE2, 15-keto-PGE1-alpha and 15-keto- PGE2-alpha with highest activity towards 15-keto-PGE2. Overexpression represses transcriptional activity of PPARG and inhibits adipocyte differentiation. Belongs to the NADP-dependent oxidoreductase L4BD family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 1.3.1.48; Oxidoreductase

Chromosomal Location of Human Ortholog: 14q24.3

Cellular Component: cytoplasm

Molecular Function: 15-oxoprostaglandin 13-oxidase activity; zinc ion binding

Biological Process: prostaglandin metabolic process