Rabbit anti-Human PTEN Induced Putative Kinase 1 (PINK1) Polyclonal Antibody | anti-PINK1 antibody

Polyclonal Antibody to PTEN Induced Putative Kinase 1 (PINK1)

Cat. Num. AAA2006545

• Gene Names: PINK1; BRPK; PARK6
• Reactivity: Human
• Applications: Immunocytochemistry, Immunohistochemistry, ELISA, Western Blot
• Purity: Affinity Chromatography
• Synonyms: PTEN Induced Putative Kinase 1 (PINK1); Polyclonal Antibody; Polyclonal Antibody to PTEN Induced Putative Kinase 1 (PINK1); anti-PINK1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: The antibody is a rabbit polyclonal antibody raised against PINK1. It has been selected for its ability to recognize PINK1 in immunohistochemical staining andwestern blotting.
• Purity/Purification: Affinity Chromatography
• Form/Format: Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.
• Concentration: 500ug/mL (varies by lot)
• Sequence: Antigen: The target protein is fused with two N-terminal Tags, His-tag and its sequence is listed below.; MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF-KRGPKQLAP HPNIIRVLRA FTSSVPLLPG ALVDYPDVLP SRLHPEGLGH GRTLFLVMKN YPCTLRQYLC VNTPSPRLAA MMLLQLLEGV DHLVQQGIAH RDLKSDNILV ELDPDGCPWL VIADFGCCLA DESIGLQLPF SSWYVDRGGN GCLMAPEVST ARPGPRAVID YSKADAWAVG AIAYEIFGLV NPFYGQGKAH LESRSYQEAQ LPALPESVPP DVRQLVRALL QREASKRPSA RVAANVL
• Sequence Length: 581

• Applicable Applications for anti-PINK1 antibody: Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB)
• Application Notes: Western blotting: 1:100-400; Immunocytochemistry in formalin fixed cells: 1:100-500; Immunohistochemistry in formalin fixed frozen section: 1:100-500; Immunohistochemistry in paraffin section: 1:50-200; Enzyme-linked Immunosorbent Assay: 1:100-200
• Immunogen: Recombinant PINK1 (Lys262~Leu507) expressed in E.coli.
• Cross Reactivity: Human, Mouse
• Conjugated Antibody: The APC conjugated antibody version of this item is also available as catalog #MBS2082525
• Preparation and Storage: Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Western Blot: Lane1: Human Serum; Lane2: Mouse Heart Tissue.)

Western Blot (WB)

(Western Blot: Sample: Recombinant PINK1, Human.)

Immunohistochemistry (IHC)

(DAB staining on IHC-P; Samples: Human Thyroid Cancer Tissue.)

Immunohistochemistry (IHC)

(DAB staining on IHC-P; Samples: Human Rectum Tissue)

Immunohistochemistry (IHC)

(DAB staining on IHC-P; Samples: Human Kidney Tissue.)

NCBI and Uniprot Product Information

• NCBI GI #: 14165272
• NCBI GeneID: 65018
• NCBI Accession #: NP_115785.1
• NCBI GenBank Nucleotide #: NM_032409.2
• UniProt Accession #: Q9BXM7; Q8N6T9; Q8NBU3; Q96DE4
• Molecular Weight: 30,104 Da
• NCBI Official Full Name: serine/threonine-protein kinase PINK1, mitochondrial
• NCBI Official Synonym Full Names: PTEN induced putative kinase 1
• NCBI Official Symbol: PINK1
• NCBI Official Synonym Symbols: BRPK; PARK6
• NCBI Protein Information: serine/threonine-protein kinase PINK1, mitochondrial
• UniProt Protein Name: Serine/threonine-protein kinase PINK1, mitochondrial
• Protein Family: Serine/threonine-protein kinase
• UniProt Gene Name: PINK1

NCBI Description

This gene encodes a serine/threonine protein kinase that localizes to mitochondria. It is thought to protect cells from stress-induced mitochondrial dysfunction. Mutations in this gene cause one form of autosomal recessive early-onset Parkinson disease. [provided by RefSeq, Jul 2008]

Uniprot Description

PINK1: Protects against mitochondrial dysfunction during cellular stress, potentially by phosphorylating mitochondrial proteins. Involved in the clearance of damaged mitochondria via selective autophagy (mitophagy). It is necessary for PARK2 recruitment to dysfunctional mitochondria to initiate their degradation. Interats with PARK2. Highly expressed in heart, skeletal muscle and testis, and at lower levels in brain, placenta, liver, kidney, pancreas, prostate, ovary and small intestine. Present in the embryonic testis from an early stage of development. Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 2.7.11.1; Kinase, protein; Membrane protein, integral; Mitochondrial; NKF2 family; Other group; Protein kinase, Other; Protein kinase, Ser/Thr (non-receptor)

Chromosomal Location of Human Ortholog: 1p36.12

Cellular Component: axon; cell body; chromatin; cytoplasm; cytoskeleton; cytosol; growth cone; integral to mitochondrial outer membrane; membrane; mitochondrial inner membrane; mitochondrial intermembrane space; mitochondrial outer membrane; mitochondrial outer membrane translocase complex; mitochondrion; nucleus; perinuclear region of cytoplasm; TORC2 complex; ubiquitin ligase complex

Molecular Function: ATP binding; calcium-dependent protein kinase activity; kinase activity; magnesium ion binding; protease activator activity; protease binding; protein binding; protein kinase activity; protein kinase B binding; protein serine/threonine kinase activity; ubiquitin protein ligase binding

Biological Process: activation of protein kinase B activity; cellular response to hydrogen sulfide; cellular response to hypoxia; cellular response to oxidative stress; establishment of protein localization to mitochondrion; macroautophagy; maintenance of protein location in mitochondrion; mitochondrion degradation; mitochondrion organization; mitochondrion to lysosome transport; negative regulation of gene expression; negative regulation of JNK cascade; negative regulation of macroautophagy; negative regulation of mitochondrial fission; negative regulation of neuron apoptosis; negative regulation of reactive oxygen species metabolic process; peptidyl-serine phosphorylation; positive regulation of ATP biosynthetic process; positive regulation of cristae formation; positive regulation of dopamine secretion; positive regulation of free ubiquitin chain polymerization; positive regulation of I-kappaB kinase/NF-kappaB signaling; positive regulation of macroautophagy; positive regulation of mitochondrial fission; positive regulation of mitophagy in response to mitochondrial depolarization; positive regulation of peptidase activity; positive regulation of peptidyl-serine phosphorylation; positive regulation of protein dephosphorylation; positive regulation of protein kinase B signaling; positive regulation of protein phosphorylation; positive regulation of protein targeting to mitochondrion; positive regulation of protein ubiquitination; positive regulation of release of cytochrome c from mitochondria; positive regulation of synaptic transmission, dopaminergic; positive regulation of transcription factor activity; positive regulation of translation; positive regulation of ubiquitin-protein transferase activity; protein amino acid phosphorylation; protein stabilization; protein ubiquitination; regulation of cellular response to oxidative stress; regulation of hydrogen peroxide metabolic process; regulation of mitochondrial membrane potential; regulation of mitochondrion organization; regulation of oxidative phosphorylation; regulation of protein complex assembly; regulation of protein ubiquitination; regulation of reactive oxygen species metabolic process; regulation of synaptic vesicle transport; response to oxidative stress; response to stress; TORC2 signaling; ubiquitin-dependent protein catabolic process

Disease: Parkinson Disease 6, Autosomal Recessive Early-onset