Rabbit anti-Human PSME1 Polyclonal Antibody | anti-PSME1 antibody

PSME1, CT (PSME1, IFI5111, Proteasome activator complex subunit 1, 11S regulator complex subunit alpha, Activator of multicatalytic protease subunit 1, Interferon gamma up-regulated I-5111 protein, Proteasome activator 28 subunit alpha) (Azide free) (HRP)

Cat. Num. AAA6338341

• Gene Names: PSME1; PA28A; IFI5111; REGalpha; PA28alpha
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: PSME1; Polyclonal Antibody; CT (PSME1; IFI5111; Proteasome activator complex subunit 1; 11S regulator complex subunit alpha; Activator of multicatalytic protease subunit 1; Interferon gamma up-regulated I-5111 protein; Proteasome activator 28 subunit alpha) (Azide free) (HRP); Proteasome activator 28 subunit alpha; anti-PSME1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-PSME1 antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:50-100; ELISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: PSME1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 219-258 amino acids from the C-terminal region of human PSME1.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(PSME1 Antibody (C-term) western blot analysis in HL-60 cell line lysates (35ug/lane). This demonstrates the PSME1 antibody detected the PSME1 protein (arrow).)

Immunohistochemistry (IHC)

(PSME1 Antibody (C-term) IHC analysis in formalin fixed and paraffin embedded hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the PSME1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(PSME1 Antibody (C-term) flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-PSME1 antibody

The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. The immunoproteasome contains an alternate regulator, referred to as the 11S regulator or PA28, that replaces the 19S regulator. Three subunits (alpha, beta and gamma) of the 11S regulator have been identified. This gene encodes the alpha subunit of the 11S regulator, one of the two 11S subunits that is induced by gamma-interferon. Three alpha and three beta subunits combine to form a heterohexameric ring.

Product Categories/Family for anti-PSME1 antibody

Antibodies; Proteasomes

References

Ruckrich, T., et al. Leukemia 23(6):1098-1105(2009). Lemaire, R., et al. J. Proteome Res. 6(11):4127-4134(2007). Kobayashi, K., et al. Brain Res. 1170, 129-139 (2007).

NCBI and Uniprot Product Information

• NCBI GI #: 528078353
• NCBI GeneID: 5720
• NCBI Accession #: NP_001268457.1
• NCBI GenBank Nucleotide #: NM_001281528.1
• UniProt Accession #: Q06323; Q6IBM2; Q9UEF4; A6NJG9; H0YNE3
• Molecular Weight: 26,870 Da
• NCBI Official Full Name: proteasome activator complex subunit 1 isoform 3
• NCBI Official Synonym Full Names: proteasome (prosome, macropain) activator subunit 1 (PA28 alpha)
• NCBI Official Symbol: PSME1
• NCBI Official Synonym Symbols: PA28A; IFI5111; REGalpha; PA28alpha
• NCBI Protein Information: proteasome activator complex subunit 1; 11S regulator complex subunit alpha; 29-kD MCP activator subunit; IGUP I-5111; activator of multicatalytic protease subunit 1; interferon gamma up-regulated I-5111 protein; interferon-gamma IEF SSP 5111; interferon-
• UniProt Protein Name: Proteasome activator complex subunit 1
• Protein Family: Proteasome activator complex
• UniProt Gene Name: PSME1
• UniProt Synonym Gene Names: IFI5111; REG-alpha; IGUP I-5111; PA28a; PA28alpha
• UniProt Entry Name: PSME1_HUMAN

NCBI Description

The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. The immunoproteasome contains an alternate regulator, referred to as the 11S regulator or PA28, that replaces the 19S regulator. Three subunits (alpha, beta and gamma) of the 11S regulator have been identified. This gene encodes the alpha subunit of the 11S regulator, one of the two 11S subunits that is induced by gamma-interferon. Three alpha and three beta subunits combine to form a heterohexameric ring. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013]

Uniprot Description

PSME1: Implicated in immunoproteasome assembly and required for efficient antigen processing. The PA28 activator complex enhances the generation of class I binding peptides by altering the cleavage pattern of the proteasome. Heterodimer of PSME1 and PSME2, which forms a hexadimeric ring. PSME1 can form homoheptamers. By IFNG/IFN-gamma. Belongs to the PA28 family.

Protein type: Proteasome complex

Chromosomal Location of Human Ortholog: 14q11.2

Cellular Component: nucleoplasm; proteasome complex; proteasome activator complex; cytoplasm; cytosol

Biological Process: positive regulation of ubiquitin-protein ligase activity during mitotic cell cycle; negative regulation of ubiquitin-protein ligase activity during mitotic cell cycle; protein polyubiquitination; viral reproduction; apoptosis; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest; regulation of apoptosis; antigen processing and presentation of peptide antigen via MHC class I; regulation of ubiquitin-protein ligase activity during mitotic cell cycle; anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; antigen processing and presentation of exogenous peptide antigen via MHC class I; gene expression; mitotic cell cycle; regulation of amino acid metabolic process; G1/S transition of mitotic cell cycle; negative regulation of apoptosis