Rabbit anti-Human PSMD4 Polyclonal Antibody | anti-PSMD4 antibody

PSMD4 (26S Proteasome Non-ATPase Regulatory Subunit 4, 26S Proteasome Regulatory Subunit S5A, 26S Proteasome Regulatory Subunit RPN10, Multiubiquitin Chain-binding Protein, Antisecretory Factor 1) (AP)

Cat. Num. AAA6391165

• Gene Names: PSMD4; AF; ASF; S5A; AF-1; MCB1; Rpn10; pUB-R5
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: PSMD4; Polyclonal Antibody; PSMD4 (26S Proteasome Non-ATPase Regulatory Subunit 4; 26S Proteasome Regulatory Subunit S5A; 26S Proteasome Regulatory Subunit RPN10; Multiubiquitin Chain-binding Protein; Antisecretory Factor 1) (AP); anti-PSMD4 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human PSMD4.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).

• Applicable Applications for anti-PSMD4 antibody: Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: Full length human PSMD4, aa1-377 (NP_002801.1).
• Immunogen Sequence: MVLESTMVCVDNSEYMRNGDFLPTRLQAQQDAVNIVCHSKTRSNPENNVGLITLANDCEVLTTLTPDTGRILSKLHTVQPKGKITFCTGIRVAHLALKHRQGKNHKMRIIAFVGSPVEDNEKDLVKLAKRLKKEKVNVDIINFGEEEVNTEKLTAFVNTLNGKDGTGSHLVTVPPGPSLADALISSPILAGEGGAMLGLGASDFEFGVDPSADPELALALRVSMEEQRQRQEEEARRAAAASAAEAGIATTGTEDSDDALLKMTISQQEFGRTGLPDLSSMTEEEQIAYAMQMSLQGAEFGQAESADIDASSAMDTSEPAKEEDDYDVMQDPEFLQSVLENLPGVDPNNEAIRNAMGSLASQATKDGKKDKKEEDKK
• Conjugate: AP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot analysis of PSMD4 expression in transfected 293T cell line by PSMD4 polyclonal antibody. Lane 1: PSMD4 transfected lysate (40.7kD). Lane 2: Non-transfected lysate.)

Related Product Information for anti-PSMD4 antibody

The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. PSDM4 one of the non-ATPase subunits of the 19S regulator lid.

Product Categories/Family for anti-PSMD4 antibody

Antibodies; Proteasomes

NCBI and Uniprot Product Information

• NCBI GI #: 5292161
• NCBI GeneID: 5710
• NCBI Accession #: NP_002801.1
• NCBI GenBank Nucleotide #: NM_002810.2
• UniProt Accession #: P55036; Q5VWC5; Q9NS92; D3DV16
• Molecular Weight: 28,612 Da
• NCBI Official Full Name: 26S proteasome non-ATPase regulatory subunit 4 isoform 2
• NCBI Official Synonym Full Names: proteasome 26S subunit, non-ATPase 4
• NCBI Official Symbol: PSMD4
• NCBI Official Synonym Symbols: AF; ASF; S5A; AF-1; MCB1; Rpn10; pUB-R5
• NCBI Protein Information: 26S proteasome non-ATPase regulatory subunit 4
• UniProt Protein Name: 26S proteasome non-ATPase regulatory subunit 4
• Protein Family: 26S proteasome non-ATPase regulatory
• UniProt Gene Name: PSMD4
• UniProt Synonym Gene Names: MCB1; AF; ASF
• UniProt Entry Name: PSMD4_HUMAN

NCBI Description

The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes one of the non-ATPase subunits of the 19S regulator lid. Pseudogenes have been identified on chromosomes 10 and 21. [provided by RefSeq, Jul 2008]

Uniprot Description

PSMD4: Binds and presumably selects ubiquitin-conjugates for destruction. Displays selectivity for longer polyubiquitin chains. Modulates intestinal fluid secretion. The 26S proteasome is composed of a core protease, known as the 20S proteasome, capped at one or both ends by the 19S regulatory complex (RC). The RC is composed of at least 18 different subunits in two subcomplexes, the base and the lid, which form the portions proximal and distal to the 20S proteolytic core, respectively. Directly interacts with NUB1. Interacts with SQSTM1. Interacts with UBQLN4. Belongs to the proteasome subunit S5A family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Protease; Proteasome complex

Chromosomal Location of Human Ortholog: 1q21.3

Cellular Component: proteasome complex; nucleoplasm; cytoplasm; cytosol

Molecular Function: identical protein binding; protein binding

Biological Process: positive regulation of ubiquitin-protein ligase activity during mitotic cell cycle; negative regulation of ubiquitin-protein ligase activity during mitotic cell cycle; protein polyubiquitination; viral reproduction; apoptosis; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest; regulation of apoptosis; antigen processing and presentation of peptide antigen via MHC class I; regulation of ubiquitin-protein ligase activity during mitotic cell cycle; anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; antigen processing and presentation of exogenous peptide antigen via MHC class I; gene expression; mitotic cell cycle; regulation of amino acid metabolic process; negative regulation of apoptosis; G1/S transition of mitotic cell cycle