Rabbit anti-Human PSMD4 Polyclonal Antibody | anti-PSMD4 antibody

PSMD4, CT (26S Proteasome Non-ATPase Regulatory Subunit 4, 26S Proteasome Regulatory Subunit S5A, 26S Proteasome Regulatory Subunit RPN10, Multiubiquitin Chain-binding Protein, Antisecretory Factor 1) (APC)

Cat. Num. AAA6494944

• Gene Names: PSMD4; AF; ASF; S5A; AF-1; MCB1; Rpn10; pUB-R5
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein G Affinity Chromatography.
• Synonyms: PSMD4; Polyclonal Antibody; CT (26S Proteasome Non-ATPase Regulatory Subunit 4; 26S Proteasome Regulatory Subunit S5A; 26S Proteasome Regulatory Subunit RPN10; Multiubiquitin Chain-binding Protein; Antisecretory Factor 1) (APC); anti-PSMD4 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human PSMD4.
• Purity/Purification: Purified by Protein G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

• Applicable Applications for anti-PSMD4 antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:50-1:100; FLISA: 1:1000; WB: 1:100-1:500; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide corresponding to a a portion within aa355-384 at the C-terminal of human PSMD4, conjugated to KLH.
• Conjugate: APC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

Western Blot (WB)

(The anti-PSMD4 Pab is used in Western blot to detect PSMD4 in Jurkat cell lysate.)

Product Categories/Family for anti-PSMD4 antibody

Antibodies; Proteasomes

References

1. Walters, K.J., et al., Biochemistry 41(6):1767-1777 (2002). 2. Kawahara, H., et al., EMBO J. 19(15):4144-4153 (2000). 3. Tanahashi, N., et al., J. Biol. Chem. 275(19):14336-14345 (2000). 4. Johansson, E., et al., J. Biol. Chem. 270(35):20615-20620 (1995). 5. Coux, O., et al., Annu. Rev. Biochem. 65, 801-847 (1996).

NCBI and Uniprot Product Information

• NCBI GI #: 1709796
• NCBI GeneID: 5710
• UniProt Accession #: P55036; Q5VWC5; Q9NS92; D3DV16
• Molecular Weight: 377
• NCBI Official Full Name: 26S proteasome non-ATPase regulatory subunit 4
• NCBI Official Synonym Full Names: proteasome (prosome, macropain) 26S subunit, non-ATPase, 4
• NCBI Official Symbol: PSMD4
• NCBI Official Synonym Symbols: AF; ASF; S5A; AF-1; MCB1; Rpn10; pUB-R5
• NCBI Protein Information: 26S proteasome non-ATPase regulatory subunit 4; angiocidin; antisecretory factor 1; S5a/antisecretory factor protein; multiubiquitin chain-binding protein; 26S proteasome regulatory subunit S5A
• UniProt Protein Name: 26S proteasome non-ATPase regulatory subunit 4
• Protein Family: 26S proteasome non-ATPase regulatory
• UniProt Gene Name: PSMD4
• UniProt Synonym Gene Names: MCB1; AF; ASF
• UniProt Entry Name: PSMD4_HUMAN

NCBI Description

The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes one of the non-ATPase subunits of the 19S regulator lid. Pseudogenes have been identified on chromosomes 10 and 21. [provided by RefSeq, Jul 2008]

Uniprot Description

PSMD4: Binds and presumably selects ubiquitin-conjugates for destruction. Displays selectivity for longer polyubiquitin chains. Modulates intestinal fluid secretion. The 26S proteasome is composed of a core protease, known as the 20S proteasome, capped at one or both ends by the 19S regulatory complex (RC). The RC is composed of at least 18 different subunits in two subcomplexes, the base and the lid, which form the portions proximal and distal to the 20S proteolytic core, respectively. Directly interacts with NUB1. Interacts with SQSTM1. Interacts with UBQLN4. Belongs to the proteasome subunit S5A family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Protease; Proteasome complex

Chromosomal Location of Human Ortholog: 1q21.3

Cellular Component: proteasome complex; nucleoplasm; cytoplasm; cytosol

Molecular Function: identical protein binding; protein binding

Biological Process: positive regulation of ubiquitin-protein ligase activity during mitotic cell cycle; negative regulation of ubiquitin-protein ligase activity during mitotic cell cycle; protein polyubiquitination; viral reproduction; apoptosis; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest; regulation of apoptosis; antigen processing and presentation of peptide antigen via MHC class I; regulation of ubiquitin-protein ligase activity during mitotic cell cycle; anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; antigen processing and presentation of exogenous peptide antigen via MHC class I; gene expression; mitotic cell cycle; regulation of amino acid metabolic process; negative regulation of apoptosis; G1/S transition of mitotic cell cycle