Western Blot (WB)
(Anti-PSMC3 rabbit polyclonal antibody at 1:500 dilution Lane A: 293T Whole Cell Lysate Lane B: HeLa Whole Cell Lysate Lane C: HepG2 Whole Cell Lysate Lane D: Jurkat Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:46 kDa Observed band size:50 kDa)
Rabbit anti-Human PSMC3 Polyclonal Antibody | anti-PSMC3 antibody
Anti-PSMC3 Antibody, Rabbit Polyclonal
Reactivity
Human
Applications
Western Blot
Purity
Protein A & Antigen Affinity
Synonyms
PSMC3; Polyclonal Antibody; Anti-PSMC3 Antibody; Rabbit Polyclonal; PSMC3 Antibody; Rabbit PAb; Antigen Affinity Purified; proteasome (prosome; macropain) 26S subunit; ATPase; 3; Anti-TBP1 Antibody; anti-PSMC3 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Human PSMC3
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; PBS, pH7.0 with 0.03% Proclin300
Applicable Applications for anti-PSMC3 antibody
Western Blot (WB)
Application Notes
WB: 1:500-1:2000
Immunogen
E Coli-derived Human PSMC3 fragment
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with E Coli-derived Human PSMC3 fragment, and purified by antigen affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Avoid repeated freeze-thaw cycles.
Western Blot (WB)
(Anti-PSMC3 rabbit polyclonal antibody at 1:500 dilution Lane A: 293T Whole Cell Lysate Lane B: HeLa Whole Cell Lysate Lane C: HepG2 Whole Cell Lysate Lane D: Jurkat Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:46 kDa Observed band size:50 kDa)
Western Blot (WB)
(Anti-PSMC3 rabbit polyclonal antibody at 1:500 dilution Lane A: 293T Whole Cell Lysate Lane B: HeLa Whole Cell Lysate Lane C: HepG2 Whole Cell Lysate Lane D: Jurkat Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:46 kDa Observed band size:50 kDa)
Related Product Information for anti-PSMC3 antibody
PSMC3 (Proteasome 26S Subunit, ATPase 3) is a Protein Coding gene. The PSMC3 gene, located on 11p11.2, is conserved in chimpanzee, Rhesus monkey, dog, cow, mouse, and rat, etc. This gene encodes one of the ATPase subunits, a member of the triple-A family of ATPases that have a chaperone-like activity. This subunit may compete with PSMC2 for binding to the HIV tat protein to regulate the interaction between the viral protein and the transcription complex. PSMC3 is a multifunctional protein, originally identified as a regulator of HIV-1-Tat mediated transcription. It is an AAA-ATPase component of the 19S regulative subunit of the proteasome and, as other members of this protein family, fulfills different cellular functions including proteolysis and transcriptional regulation. Diseases associated with PSMC3 include Type 1 Diabetes Mellitus 11 and Angelman Syndrome.
References
Sakao Y, et al. (2000) Mouse proteasomal atpases psmc3 and psmc4: Genomic organization and gene targeting. Genomics 67 (1): 1-7.Pakay JL, et al. (2012) A 19s proteasomal subunit cooperates with an erk mapk-regulated degron to regulate accumulation of fra-1 in tumour cells. Oncogene 31 (14): 1817-1824.(2019) Retraction: A regulatory mechanism involving tbp-1/tat-binding protein 1 and akt/pkb in the control of cell proliferation. PLoS One 14 (9): e0222287.
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Product Notes
The PSMC3 (Catalog #AAA8108143) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-PSMC3 Antibody, Rabbit Polyclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PSMC3 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:500-1:2000. Researchers should empirically determine the suitability of the PSMC3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PSMC3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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