Goat PSMB5 / MB1 Polyclonal Antibody | anti-PSMB5 antibody

Goat Polyclonal to Human PSMB5 / MB1

Cat. Num. AAA244007

• Gene Names: PSMB5; X; MB1; LMPX
• Reactivity: Bat, Gibbon, Bovine, Dog, Xenopus, Gorilla, Hamster, Horse, Human, Monkey, Mouse, Orangutan, Pig, Rabbit, Rat, Zebrafish; Predicted Reactivity: Insect (at least 90% immunogen sequence identity)
• Applications: Immunohistochemistry
• Purity: Immunoaffinity Purified
• Synonyms: PSMB5 / MB1; Polyclonal Antibody; Goat Polyclonal to Human PSMB5 / MB1; Anti-PSMB5 / MB1 Antibody IHC-plus; PSMB5; Proteasome chain 6; Proteasome subunit MB1; Proteasome subunit X; Proteasome catalytic subunit 3; Macropain epsilon chain; MB1; LMPX; Proteasome beta 5 subunit; Proteasome epsilon chain; Proteasome subunit beta type-5; Human PSMB5; anti-PSMB5 antibody

• Host: Goat
• Reactivity: Bat, Gibbon, Bovine, Dog, Xenopus, Gorilla, Hamster, Horse, Human, Monkey, Mouse, Orangutan, Pig, Rabbit, Rat, Zebrafish; Predicted Reactivity: Insect (at least 90% immunogen sequence identity)
• Clonality: Polyclonal
• Specificity: Human PSMB5.
• Purity/Purification: Immunoaffinity Purified
• Form/Format: Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
• Concentration: 0.5 mg/ml (varies by lot)
• Sequence Length: 263

• Applicable Applications for anti-PSMB5 antibody: Immunohistochemistry (IHC - Paraffin)
• Application Notes: IHC-P (3.75 ug/ml); Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary anti...
• Target Species: Human
• Immunogen Description: Synthetic peptide from human PSMB5 / MB1.
• Immunogen: PSMB5 / MB1 antibody was raised against synthetic peptide from human PSMB5 / MB1. Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Panda, Dog, Bat, Bovine, Horse, Rabbit, Pig, Opossum, Platypus, Xenopus, Catfish, Smelt, Stickleback, Pufferfish, Zebrafish, Sea anemone (100%); Salmon, Seq squirt, Mosquito, Water flea, Capsaspora (92%); Chicken, Trout, Ant, Beetle, Trichoplax, Tick, Tunicate (83%).
• Preparation and Storage: Store at -20 degree C. Minimize freezing and thawing.

Immunohistochemistry (IHC)

(Anti-PSMB5 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 3.75 ug/ml.)

Immunohistochemistry (IHC)

(Anti-PSMB5 antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 3.75 ug/ml.)

Product Categories/Family for anti-PSMB5 antibody

Family: Protease; Subfamily: Threonine T1

NCBI and Uniprot Product Information

• NCBI GI #: 4506201
• NCBI GeneID: 5693
• NCBI Accession #: NP_002788.1
• NCBI GenBank Nucleotide #: NM_002797.4
• UniProt Accession #: P28074; Q16242; Q6PEW2; Q7Z3B5; Q86T01; Q9TNN9; B2R4N9; B4DUM9; D3DS43; E9PAV2
• Molecular Weight: 17,782 Da
• NCBI Official Full Name: proteasome subunit beta type-5 isoform 1
• NCBI Official Synonym Full Names: proteasome (prosome, macropain) subunit, beta type, 5
• NCBI Official Symbol: PSMB5
• NCBI Official Synonym Symbols: X; MB1; LMPX
• NCBI Protein Information: proteasome subunit beta type-5; proteasome chain 6; proteasome subunit X; proteasome subunit MB1; macropain epsilon chain; proteasome epsilon chain; proteasome beta 5 subunit; proteasome catalytic subunit 3; proteasome subunit, beta type, 5; PSX large multifunctional protease X; multicatalytic endopeptidase complex epsilon chain
• UniProt Protein Name: Proteasome subunit beta type-5
• Protein Family: Proteasome
• UniProt Gene Name: PSMB5
• UniProt Synonym Gene Names: LMPX; MB1; X
• UniProt Entry Name: PSB5_HUMAN

NCBI Description

The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit in the proteasome. This catalytic subunit is not present in the immunoproteasome and is replaced by catalytic subunit 3i (proteasome beta 8 subunit). Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2009]

Uniprot Description

PSMB5: The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. This unit is responsible of the chymotrypsin-like activity of the proteasome and is one of the principal target of the proteasome inhibitor bortezomib. May catalyze basal processing of intracellular antigens. Plays a role in the protection against oxidative damage through the Nrf2-ARE pathway. Belongs to the peptidase T1B family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Protease; Proteasome complex; EC 3.4.25.1

Chromosomal Location of Human Ortholog: 14q11.2

Cellular Component: proteasome complex; nucleoplasm; proteasome core complex; centrosome; nucleus; cytosol

Molecular Function: threonine endopeptidase activity; protein binding

Biological Process: positive regulation of ubiquitin-protein ligase activity during mitotic cell cycle; negative regulation of ubiquitin-protein ligase activity during mitotic cell cycle; protein polyubiquitination; viral reproduction; apoptosis; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest; regulation of apoptosis; antigen processing and presentation of peptide antigen via MHC class I; regulation of ubiquitin-protein ligase activity during mitotic cell cycle; anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; antigen processing and presentation of exogenous peptide antigen via MHC class I; gene expression; response to oxidative stress; mitotic cell cycle; regulation of amino acid metabolic process; negative regulation of apoptosis; G1/S transition of mitotic cell cycle