Rabbit PSMA1 Polyclonal Antibody | anti-PSMA1 antibody

PSMA1 (Proteasome Alpha 1, NU, HC2, PROS30) (Biotin)

Cat. Num. AAA6433483

• Gene Names: PSMA1; NU; HC2; PROS30; HEL-S-275
• Reactivity: Human, Mouse, Rat
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by affinity chromatography.
• Synonyms: PSMA1; Polyclonal Antibody; PSMA1 (Proteasome Alpha 1; NU; HC2; PROS30) (Biotin); anti-PSMA1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes PSMA1. Species Crossreactivity: human, mouse, rat
• Purity/Purification: Purified by affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.
• Concentration: ~1mg/ml (varies by lot)

• Applicable Applications for anti-PSMA1 antibody: Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: IHC: 1:50-1:200; WB: 1:500-1:2000; Applications are based on unconjugated antibody.
• Immunogen: Recombinant protein corresponding to human PSMA1.
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of extracts of various cell lines, using PSMA1 antibody.)

Immunohistochemistry (IHC)

(Immunohistochemical of paraffin-embedded human breast cancer using PSMA1 antibody at dilution of 1:100 (400x lens).)

Immunohistochemistry (IHC)

(Immunohistochemical of paraffin-embedded mouse heart using PSMA1 antibody at dilution of 1:100 (400x lens).)

Immunohistochemistry (IHC)

(Immunohistochemical of paraffin-embedded human stomach cancer using PSMA1 antibody at dilution of 1:100 (400x lens).)

Immunohistochemistry (IHC)

(Immunohistochemical of paraffin-embedded rat lung using PSMA1 antibody at dilution of 1:100 (200x lens).)

Product Categories/Family for anti-PSMA1 antibody

Antibodies; Proteasomes

NCBI and Uniprot Product Information

• NCBI GI #: 130848
• NCBI GeneID: 5682
• UniProt Accession #: P25786; Q53YE8; Q9BRV9; A8K400
• Molecular Weight: 263
• NCBI Official Full Name: Proteasome subunit alpha type-1
• NCBI Official Synonym Full Names: proteasome (prosome, macropain) subunit, alpha type, 1
• NCBI Official Symbol: PSMA1
• NCBI Official Synonym Symbols: NU; HC2; PROS30; HEL-S-275
• NCBI Protein Information: proteasome subunit alpha type-1; PROS-30; protein P30-33K; proteasome nu chain; macropain subunit C2; macropain subunit nu; proteasome subunit nu; 30 kDa prosomal protein; proteasome component C2; proteasome subunit, alpha-type, 1; epididymis secretory pr
• UniProt Protein Name: Proteasome subunit alpha type-1
• Protein Family: Proteasome
• UniProt Gene Name: PSMA1
• UniProt Synonym Gene Names: HC2; NU; PROS30; PSC2; PROS-30
• UniProt Entry Name: PSA1_HUMAN

NCBI Description

The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the peptidase T1A family, that is a 20S core alpha subunit. Alternative splicing results in multiple transcript variants encoding distinct isoforms.[provided by RefSeq, Jan 2009]

Uniprot Description

PSMA1: The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. Mediates the lipopolysaccharide-induced signal transduction in the macrophage proteasome. Might be involved in the anti-inflammatory response of macrophages during the interaction with C.albicans heat-inactivated cells. Belongs to the peptidase T1A family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.4.25.1; Protease; Proteasome complex

Chromosomal Location of Human Ortholog: 11p15.1

Cellular Component: nucleoplasm; proteasome complex; polysome; proteasome core complex; centrosome; cytosol; nucleus

Molecular Function: threonine endopeptidase activity; protein binding; RNA binding

Biological Process: positive regulation of ubiquitin-protein ligase activity during mitotic cell cycle; negative regulation of ubiquitin-protein ligase activity during mitotic cell cycle; protein polyubiquitination; viral reproduction; apoptosis; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest; regulation of apoptosis; antigen processing and presentation of peptide antigen via MHC class I; regulation of ubiquitin-protein ligase activity during mitotic cell cycle; anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; antigen processing and presentation of exogenous peptide antigen via MHC class I; gene expression; mitotic cell cycle; regulation of amino acid metabolic process; negative regulation of apoptosis; G1/S transition of mitotic cell cycle