Immunofluorescence (IF)
(MBS9602213 staining A549 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
Rabbit anti-Human PSK-H1 Polyclonal Antibody | anti-PSK-H1 antibody
PSK-H1 Antibody
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
PSK-H1; Polyclonal Antibody; PSK-H1 Antibody; KPSH1_HUMAN; Protein serine kinase H1; PSK H1; PSKH1; Serine/threonine protein kinase H1; Serine/threonine-protein kinase H1; anti-PSK-H1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
PSK-H1 antibody detects endogenous levels of total PSK-H1
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
424
Applicable Applications for anti-PSK-H1 antibody
Western Blot (WB), Immunohistochemisty (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA)
Application Notes
WB: 1:1000
IHC: 1:200
IF/ICC: 1:100-1:500
IHC: 1:200
IF/ICC: 1:100-1:500
Immunogen
A synthesized peptide derived from human PSK-H1
Subcellular Location
Golgi Apparatus. Cytoplasm > Cytoskeleton > Centrosome. Nucleus Speckle. Endoplasmic Reticulum Membrane. Cell Membrane. Cytoplasm. Localized in the brefeldin A-sensitive golgi compartment, at centrosomes, in the nucleus with a somewhat speckle-like presence, membrane-associated to the endoplasmic reticulum (ER) and the plasma membrane (PM), and more diffusely in the cytoplasm. Found to concentrate in splicing factor compartments (SFCs) within the nucleus of interphase cells. The acylation-negative form may be only cytoplasmic and nuclear. Acylation seems to allow the sequestering to the intracellular membranes. Myristoylation may mediate targeting to the intracellular non-golgi membranes and palmitoylation may mediate the targeting to the golgi membranes. Dual acylation is required to stabilize the interaction with golgi membranes.
Tissue Specificity
Expressed in all tissues and cell lines tested with the highest level of abundance in testis.
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Immunofluorescence (IF)
(MBS9602213 staining A549 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
Immunofluorescence (IF)
(MBS9602213 staining A549 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
Western Blot (WB)
(Western blot analysis of PSK-H1 using A549 whole cell lysates)
Western Blot (WB)
(Western blot analysis of PSK-H1 using A549 whole cell lysates)
Immunohistochemistry (IHC)
(MBS9602213 at 1/100 staining Human lung tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Immunohistochemistry (IHC)
(MBS9602213 at 1/100 staining Human lung tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Related Product Information for anti-PSK-H1 antibody
Function: May be a SFC-associated serine kinase (splicing factor compartment-associated serine kinase) with a role in intranuclear SR protein (non-snRNP splicing factors containing a serine/arginine-rich domain) trafficking and pre-mRNA processing.
Subunit Structure: Homodimer.
Post-translational Modifications: Autophosphorylated on serine residues. Myristoylated. Required for membrane association. Prerequisite for palmitoylation to occur. Palmitoylated.
Similarity: Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.
Subunit Structure: Homodimer.
Post-translational Modifications: Autophosphorylated on serine residues. Myristoylated. Required for membrane association. Prerequisite for palmitoylation to occur. Palmitoylated.
Similarity: Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 48 kDa
Predicted: 49 kDa
Predicted: 49 kDa
NCBI Official Full Name
serine/threonine-protein kinase H1
NCBI Official Synonym Full Names
protein serine kinase H1
NCBI Official Symbol
PSKH1
NCBI Protein Information
serine/threonine-protein kinase H1
UniProt Protein Name
Serine/threonine-protein kinase H1
UniProt Gene Name
PSKH1
UniProt Synonym Gene Names
PSK-H1
Uniprot Description
May be a SFC-associated serine kinase (splicing factor compartment-associated serine kinase) with a role in intranuclear SR protein (non-snRNP splicing factors containing a serine/arginine-rich domain) trafficking and pre-mRNA processing.
Research Articles on PSK-H1
Similar Products
Product Notes
The PSK-H1 pskh1 (Catalog #AAA9602213) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PSK-H1 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PSK-H1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemisty (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA). WB: 1:1000 IHC: 1:200 IF/ICC: 1:100-1:500. Researchers should empirically determine the suitability of the PSK-H1 pskh1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PSK-H1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
