Goat Pseudomonas aeruginosa Exotoxin A Polyclonal Antibody
Pseudomonas aeruginosa Exotoxin A
Applications
ELISA, Western Blot
Purity
Serum
Synonyms
Pseudomonas aeruginosa Exotoxin A; Polyclonal Antibody; Anti -Pseudomonas aeruginosa Exotoxin A; anti-Pseudomonas aeruginosa Exotoxin A antibody
Host
Goat
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes Pseudomonas aeruginosa Exotoxin A.
Purity/Purification
Serum
Form/Format
Supplied as a sterile-filtered lyophilized powder. Reconstitute with 500ul sterile ddH2O. Do not vortex.
Applicable Applications for anti-Pseudomonas aeruginosa Exotoxin A antibody
Suitable for use in ELISA and Western Blot. Other applications not tested.
Application Notes
Western Blot: 1:200,000
Optimal dilutions to be determined by the researcher.
Optimal dilutions to be determined by the researcher.
Immunogen
Exotoxin A prepared from P. aeruginosa.
Titer
ELISA: The titer measures at mid-point was 1:178,000 using 2ug coating of Endotoxin A.
Preparation and Storage
Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstitute with seterile ddH20. Aliquot to avoid repeated freezing and thawing. Store at -20°C For maximum recovery, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Related Product Information for anti-Pseudomonas aeruginosa Exotoxin A antibody
Exotoxin A is one of the toxic proteins released by pathogenic strains of Pseudomonas aeruginosa. It is secreted as a proenzyme with a molecular weight of 66kD. Like diphtheria toxin, exotoxin A is translocated into susceptible mammalian cells. Covalent alteration of the molecule occurs, rendering it enzymatically active. The intact toxin can be activated 20- to 50-fold in vitro by treatment with urea and dithiothreitol. Exotoxin A catalyzes the transfer of the adenosine diphosphate ribose moiety of oxidized nicotinamide adenine dinucleotide onto elongation factor 2(3): NAD + EF-2 ® ADP-ribose-EF-2 + nicotinamide + H+. This ADP-ribosylation of EF-2 blocks polypeptide assembly on the surface of the ribosome. As a result, this molecule is cytopathic for a number of cultured cell lines and is toxic to animals. Although exotoxin A and diphtheria toxin have identical enzymatic activity, they exhibit distinct target cell specificities and are immunologically unrelated. In crude preparations or in purified material which has been stored, lower molecular weight peptides with ADP-ribosylating activity may be present. Similarly, after reduction and limited proteolytic cleavage with chymotrypsin, a 26,000 dalton fragment that has enzymatic activity may be separated. This fragment alone is not toxic to intact cells.
Product Categories/Family for anti-Pseudomonas aeruginosa Exotoxin A antibody
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Product Notes
The Pseudomonas aeruginosa Exotoxin A (Catalog #AAA611247) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Pseudomonas aeruginosa Exotoxin A can be used in a range of immunoassay formats including, but not limited to, Suitable for use in ELISA and Western Blot. Other applications not tested. . Western Blot: 1:200,000 Optimal dilutions to be determined by the researcher. Researchers should empirically determine the suitability of the Pseudomonas aeruginosa Exotoxin A for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Pseudomonas aeruginosa Exotoxin A, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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