Western Blot (WB)
(PRSS3 Antibody (N-term) western blot analysis in MDA-MB231 cell line lysates (35ug/lane).This demonstrates the PRSS3 antibody detected the PRSS3 protein (arrow).)
Rabbit anti-Human PRSS3 Polyclonal Antibody | anti-PRSS3 antibody
PRSS3, NT (PRSS3, PRSS4, TRY3, TRY4, Trypsin-3, Brain trypsinogen, Mesotrypsinogen, Serine protease 3, Serine protease 4, Trypsin III, Trypsin IV)
Purified by Protein A affinity chromatography.
Purified by Protein A affinity chromatography.
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500
Immunohistochemistry: 1:50-100
Flow Cytometry: 1:10-50
Western Blot (WB)
(PRSS3 Antibody (N-term) western blot analysis in MDA-MB231 cell line lysates (35ug/lane).This demonstrates the PRSS3 antibody detected the PRSS3 protein (arrow).)
Western Blot (WB)
(PRSS3 Antibody (N-term) western blot analysis in MDA-MB231 cell line lysates (35ug/lane).This demonstrates the PRSS3 antibody detected the PRSS3 protein (arrow).)
Immunohistochemistry (IHC)
(PRSS3 antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human pancreas tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the PRSS3 antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(PRSS3 antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human pancreas tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the PRSS3 antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Flow Cytometry (FC/FACS)
(PRSS3 Antibody (N-term) flow cytometric analysis of MDA-MB231 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Flow Cytometry (FC/FACS)
(PRSS3 Antibody (N-term) flow cytometric analysis of MDA-MB231 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a trypsinogen, which is a member of the trypsin family of serine proteases. This enzyme is expressed in the brain and pancreas and is resistant to common trypsin inhibitors. It is active on peptide linkages involving the carboxyl group of lysine or arginine. This gene is localized to the locus of T cell receptor beta variable orphans on chromosome 9. Four transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Oct 2010]
Uniprot Description
Function: Digestive protease specialized for the degradation of trypsin inhibitors. In the ileum, may be involved in defensin processing, including DEFA5. Ref.7 Ref.8
Catalytic activity: Preferential cleavage: Arg-|-Xaa, Lys-|-Xaa.
Cofactor: Binds 1 calcium ion per subunit.
Subcellular location: Secreted.
Tissue specificity: Expressed in pancreas and brain. Also expressed in Paneth cells, at the base of small intestinal crypts. Ref.7
Sequence similarities: Belongs to the peptidase S1 family.Contains 1 peptidase S1 domain.
Research Articles on PRSS3
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Product Notes
The PRSS3 prss3 (Catalog #AAA647034) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PRSS3, NT (PRSS3, PRSS4, TRY3, TRY4, Trypsin-3, Brain trypsinogen, Mesotrypsinogen, Serine protease 3, Serine protease 4, Trypsin III, Trypsin IV) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PRSS3 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS). Suitable for use in Western Blot, Immunohistochemistry, Flow Cytometry, ELISA Dilution: ELISA: 1:1,000 Western Blot: 1:100-500 Immunohistochemistry: 1:50-100 Flow Cytometry: 1:10-50. Researchers should empirically determine the suitability of the PRSS3 prss3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PRSS3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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