Western Blot (WB)
(Western Blot analysis of PRPF6 expression in transfected 293T cell line by PRPF6 polyclonal antibody. Lane 1: C20orf14 transfected lysate (103.51kD). Lane 2: Non-transfected lysate.)
Mouse anti-Human PRPF6 Polyclonal Antibody | anti-PRPF6 antibody
PRPF6 (C20orf14, Pre-mRNA-processing Factor 6, Androgen Receptor N-terminal Domain-transactivating Protein 1, PRP6 Homolog, U5 snRNP-associated 102kD Protein)
Purified by Protein A affinity chromatography.
Purified by Protein A affinity chromatography.
Western Blot (WB)
(Western Blot analysis of PRPF6 expression in transfected 293T cell line by PRPF6 polyclonal antibody. Lane 1: C20orf14 transfected lysate (103.51kD). Lane 2: Non-transfected lysate.)
Western Blot (WB)
(Western Blot analysis of PRPF6 expression in transfected 293T cell line by PRPF6 polyclonal antibody. Lane 1: C20orf14 transfected lysate (103.51kD). Lane 2: Non-transfected lysate.)
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene appears to be involved in pre-mRNA splicing, possibly acting as a bridging factor between U5 and U4/U6 snRNPs in formation of the spliceosome. The encoded protein also can bind androgen receptor, providing a link between transcriptional activation and splicing. [provided by RefSeq, Jul 2008]
Uniprot Description
PRPF6: Involved in pre-mRNA splicing. May act in the tri-snRNP complex as a bridging factor between U5 and U4/U6 snRNPs in the late step of spliceosome assembly. May be necessary for tri-snRNP formation. Enhances dihydrotestosterone-induced transactivation activity of AR, as well as dexamethasone-induced transactivation activity of NR3C1, but does not affect estrogen-induced transactivation. Defects in PRPF6 may be the cause of retinitis pigmentosa type 60 (RP60). A retinal dystrophy belonging to the group of pigmentary retinopathies. Retinitis pigmentosa is characterized by retinal pigment deposits visible on fundus examination and primary loss of rod photoreceptor cells followed by secondary loss of cone photoreceptors. Patients typically have night vision blindness and loss of midperipheral visual field. As their condition progresses, they lose their far peripheral visual field and eventually central vision as well. Cells from RP60 patients show intron retention for pre-mRNA bearing specific splicing signals. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: RNA-binding; Spliceosome; Motility/polarity/chemotaxis; RNA splicing; Nuclear receptor co-regulator
Chromosomal Location of Human Ortholog: 20q13.33
Cellular Component: nucleoplasm; spliceosome; membrane; U4/U6 x U5 tri-snRNP complex; snRNP U5; nuclear speck; nucleus
Molecular Function: ribonucleoprotein binding; protein binding; androgen receptor binding; RNA binding; transcription coactivator activity
Biological Process: RNA splicing, via transesterification reactions; assembly of spliceosomal tri-snRNP; nuclear mRNA splicing, via spliceosome; RNA localization; RNA splicing; spliceosome assembly; gene expression; positive regulation of transcription from RNA polymerase II promoter
Disease: Retinitis Pigmentosa 60
Research Articles on PRPF6
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Product Notes
The PRPF6 prpf6 (Catalog #AAA6007728) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The PRPF6 (C20orf14, Pre-mRNA-processing Factor 6, Androgen Receptor N-terminal Domain-transactivating Protein 1, PRP6 Homolog, U5 snRNP-associated 102kD Protein) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PRPF6 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Suitable for use in Western Blot. Researchers should empirically determine the suitability of the PRPF6 prpf6 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: MNKKKKPFLG MPAPLGYVPG LGRGATGFTT RSDIGPARDA NDPVDDRHAP PGKRTVGDQM KKNQAADDDD EDLNDTNYDE FNGYAGSLFS SGPYEKDDEE ADAIYAALDK RMDERRKERR EQREKEEIEK YRMERPKIQQ QFSDLKRKLA EVTEEEWLSI PEVGDARNKR QRNPRYEKLT PVPDSFFAKH LQTGENHTSV DPRQTQFGGL NTPYPGGLNT PYPGGMTPGL MTPGTGELDM RKIGQARNTL MDMRLSQVSD SVSGQTVVDP KGYLTDLNSM IPTHGGDIND IKKARLLLKS VRETNPHHPP AWIASARLEE VTGKLQVARN LIMKGTEMCP KSEDVWLEAA RLQPGDTAKA VVAQAVRHLP QSVRIYIRAA ELETDIRAKK RVLRKALEHV PNSVRLWKAA VELEEPEDAR IMLSRAVECC PTSVELWLAL ARLETYENAR KVLNKARENI PTDRHIWITA AKLEEANGNT QMVEKIIDRA ITSLRANGVE INREQWIQDA EECDRAGSVA TCQAVMRAVI GIGIEEEDRK HTWMEDADSC VAHNALECAR AIYAYALQVF PSKKSVWLRA AYFEKNHGTR ESLEALLQRA VAHCPKAEVL WLMGAKSKWL AGDVPAARSI LALAFQANPN SEEIWLAAVK LESENDEYER ARRLLAKARS SAPTARVFMK SVKLEWVQDN IRAAQDLCEE ALRHYEDFPK LWMMKGQIEE QKEMMEKARE AYNQGLKKCP HSTPLWLLLS RLEEKIGQLT RARAILEKSR LKNPKNPGLW LESVRLEYRA GLKNIANTLM AKALQECPNS GILWSEAIFL EARPQRRTKS VDALKKCEHD PHVLLAVAKL FWSQRKITKA REWFHRTVKI DSDLGDAWAF FYKFELQHGT EEQQEEVRKR CESAEPRHGE LWCAVSKDIA NWQKKIGDIL RLVAGRIKNT F. It is sometimes possible for the material contained within the vial of "PRPF6, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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