Western Blot (WB)
(Western Blot: Sample: Recombinant PCDH15, Mouse.)
Rabbit anti-Mouse Protocadherin 15 Polyclonal Antibody | anti-PCDH15 antibody
Protocadherin 15 (PCDH15) Polyclonal Antibody
Immunohistochemistry in formalin fixed frozen section: 1:50-500
Enzyme-linked Immunosorbent Assay: 1:100-200
Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.
5uL per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control.Not used for other purposes.
Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol,BPB 0.01%, NaN3 0.02%.
Western Blot (WB)
(Western Blot: Sample: Recombinant PCDH15, Mouse.)
Western Blot (WB)
(Western Blot: Sample: Recombinant PCDH15, Mouse.)
NCBI and Uniprot Product Information
Uniprot Description
PCDH15: Calcium-dependent cell-adhesion protein. Essential for maintenance of normal retinal and cochlear function. Defects in PCDH15 are the cause of Usher syndrome type 1F (USH1F). USH is a genetically heterogeneous condition characterized by the association of retinitis pigmentosa and sensorineural deafness. Age at onset and differences in auditory and vestibular function distinguish Usher syndrome type 1 (USH1), Usher syndrome type 2 (USH2) and Usher syndrome type 3 (USH3). USH1 is characterized by profound congenital sensorineural deafness, absent vestibular function and prepubertal onset of progressive retinitis pigmentosa leading to blindness. Defects in PCDH15 are a cause of Usher syndrome type 1D/F (USH1DF). USH1DF patients are heterozygous for mutations in CDH23 and PCDH15, indicating a digenic inheritance pattern. Defects in PCDH15 are the cause of deafness autosomal recessive type 23 (DFNB23). DFNB23 is a form of sensorineural hearing loss. Sensorineural deafness results from damage to the neural receptors of the inner ear, the nerve pathways to the brain, or the area of the brain that receives sound information. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Cell adhesion; Cell development/differentiation; Membrane protein, integral
Chromosomal Location of Human Ortholog: 10 B5.3|10 37.43 cM
Cellular Component: cytoplasm; extracellular region; extracellular space; integral component of membrane; integral component of plasma membrane; membrane; photoreceptor outer segment; plasma membrane; stereocilium; stereocilium bundle; synapse
Molecular Function: calcium ion binding; protein binding; protein complex binding; protein N-terminus binding
Biological Process: actin filament bundle formation; actin filament organization; adult locomotory behavior; adult walking behavior; auditory receptor cell differentiation; auditory receptor cell stereocilium organization and biogenesis; cell adhesion; cell-cell signaling; detection of mechanical stimulus involved in equilibrioception; detection of mechanical stimulus involved in sensory perception of sound; equilibrioception; homophilic cell adhesion; inner ear development; inner ear receptor stereocilium organization and biogenesis; locomotory behavior; morphogenesis of an epithelium; multicellular organism growth; photoreceptor cell maintenance; response to calcium ion; righting reflex; sensory perception of light stimulus; sensory perception of sound; startle response; visual perception
Research Articles on PCDH15
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Product Notes
The PCDH15 pcdh15 (Catalog #AAA2032594) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Protocadherin 15 (PCDH15) Polyclonal Antibody reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Protocadherin 15 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA). Western Blot: 1:50-400 Immunohistochemistry in formalin fixed frozen section: 1:50-500 Enzyme-linked Immunosorbent Assay: 1:100-200. Researchers should empirically determine the suitability of the PCDH15 pcdh15 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Protocadherin 15, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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