Western Blot (WB)
(Western Blot: Sample: Mouse Cerebrum lysate; Primary Ab: 1ug/ml Rabbit Anti-Mouse PTK2b Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )
Rabbit anti-Mouse Protein Tyrosine Kinase 2 Beta Polyclonal Antibody | anti-PTK2b antibody
Protein Tyrosine Kinase 2 Beta (PTK2b) Polyclonal Antibody
Immunohistochemistry in formalin fixed frozen section: 1:50-500
Enzyme-linked Immunosorbent Assay: 1:100-200
Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.
5uL per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control.Not used for other purposes.
Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol,BPB 0.01%, NaN3 0.02%.
Western Blot (WB)
(Western Blot: Sample: Mouse Cerebrum lysate; Primary Ab: 1ug/ml Rabbit Anti-Mouse PTK2b Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )
Western Blot (WB)
(Western Blot: Sample: Mouse Cerebrum lysate; Primary Ab: 1ug/ml Rabbit Anti-Mouse PTK2b Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )
Western Blot (WB)
(Western Blot: Sample: Rat Cerebrum lysate; Primary Ab: 1ug/ml Rabbit Anti-Mouse PTK2b Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (#MBS2086047))
Western Blot (WB)
(Western Blot: Sample: Rat Cerebrum lysate; Primary Ab: 1ug/ml Rabbit Anti-Mouse PTK2b Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (#MBS2086047))
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Immunohistochemistry (IHC)
(DAB staining on fromalin fixed paraffin- embedded Kidney tissue))
Immunohistochemistry (IHC)
(DAB staining on fromalin fixed paraffin- embedded Kidney tissue))
NCBI and Uniprot Product Information
Uniprot Description
Pyk2: a nonreceptor tyrosine kinase of the Fak family. Predominantly expressed in the cells derived from hematopoietic lineages and in the central nervous system. Pyk2 is one of the signaling mediators for G-protein-coupled receptors. Involved in calcium induced regulation of ion channel and activation of the map kinase signaling pathway. Interacts with the SH2 domain of Grb2. May phosphorylate the voltage-gated potassium channel protein Kv1.2. Its activation is highly correlated with the stimulation of c-Jun N-terminal kinase activity. It plays an important role in cell motility such as spreading and migration. Two alternatively spliced isoforms have been described.
Protein type: EC 2.7.10.2; Fak family; Kinase, protein; Nuclear receptor co-regulator; Protein kinase, TK; Protein kinase, tyrosine (non-receptor); TK group
Chromosomal Location of Human Ortholog: 14 D1|14 34.36 cM
Cellular Component: axon; cell body; cell cortex; cell junction; cell projection; cell soma; cytoplasm; cytoskeleton; dendrite; dendritic spine; extrinsic component of cytoplasmic side of plasma membrane; focal adhesion; growth cone; lamellipodium; membrane; membrane raft; NMDA selective glutamate receptor complex; nucleus; perinuclear region of cytoplasm; plasma membrane; postsynaptic density
Molecular Function: 3-phosphoinositide-dependent protein kinase binding; ATP binding; calmodulin-dependent protein kinase activity; enzyme binding; kinase activity; NMDA glutamate receptor activity; non-membrane spanning protein tyrosine kinase activity; nucleotide binding; protein binding; protein complex binding; protein kinase activity; protein tyrosine kinase activity; receptor binding; signal transducer activity; transferase activity; ubiquitin protein ligase binding
Biological Process: actin filament organization; activation of JAK protein; adaptive immune response; angiogenesis; blood vessel endothelial cell migration; bone resorption; cell adhesion; cell surface receptor signaling pathway; cellular defense response; cellular response to fluid shear stress; cellular response to retinoic acid; chemokine-mediated signaling pathway; elevation of cytosolic calcium ion concentration; epidermal growth factor receptor signaling pathway; focal adhesion assembly; immune system process; innate immune response; integrin-mediated signaling pathway; ionotropic glutamate receptor signaling pathway; MAPK cascade; marginal zone B cell differentiation; negative regulation of apoptosis; negative regulation of bone mineralization; negative regulation of cell proliferation; negative regulation of muscle cell apoptotic process; negative regulation of myeloid cell differentiation; negative regulation of neuron apoptosis; negative regulation of ossification; negative regulation of potassium ion transport; neuron projection development; oocyte maturation; peptidyl-tyrosine autophosphorylation; peptidyl-tyrosine phosphorylation; phosphorylation; positive regulation of actin filament polymerization; positive regulation of angiogenesis; positive regulation of B cell chemotaxis; positive regulation of cell growth; positive regulation of cell migration; positive regulation of cell proliferation; positive regulation of cell-matrix adhesion; positive regulation of cellular protein metabolic process; positive regulation of DNA biosynthetic process; positive regulation of endothelial cell migration; positive regulation of ERK1 and ERK2 cascade; positive regulation of excitatory postsynaptic potential; positive regulation of JNK cascade; positive regulation of JUN kinase activity; positive regulation of neuron projection development; positive regulation of nitric oxide biosynthetic process; positive regulation of nitric-oxide synthase activity; positive regulation of peptidyl-tyrosine phosphorylation; positive regulation of phosphatidylinositol 3-kinase activity; positive regulation of protein kinase activity; positive regulation of protein ubiquitination involved in ubiquitin-dependent protein catabolic process; positive regulation of reactive oxygen species metabolic process; positive regulation of synaptic transmission, glutamatergic; positive regulation of translation; protein amino acid phosphorylation; regulation of actin cytoskeleton reorganization; regulation of angiogenesis; regulation of bone mineralization; regulation of calcium-mediated signaling; regulation of cell adhesion; regulation of cell migration; regulation of cell proliferation; regulation of cell shape; regulation of cGMP biosynthetic process; regulation of cGMP-mediated signaling; regulation of establishment of cell polarity; regulation of inositol trisphosphate biosynthetic process; regulation of leukocyte chemotaxis; regulation of macrophage chemotaxis; regulation of nitric oxide biosynthetic process; regulation of NMDA receptor activity; regulation of protein ubiquitination involved in ubiquitin-dependent protein catabolic process; regulation of release of sequestered calcium ion into cytosol; response to hydrogen peroxide; signal complex assembly; signal transduction; sprouting angiogenesis; stress fiber assembly; tumor necrosis factor-mediated signaling pathway; vascular endothelial growth factor receptor signaling pathway
Research Articles on PTK2b
Similar Products
Product Notes
The PTK2b ptk2b (Catalog #AAA2032150) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Protein Tyrosine Kinase 2 Beta (PTK2b) Polyclonal Antibody reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Protein Tyrosine Kinase 2 Beta can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA). Western Blot: 1:50-400 Immunohistochemistry in formalin fixed frozen section: 1:50-500 Enzyme-linked Immunosorbent Assay: 1:100-200. Researchers should empirically determine the suitability of the PTK2b ptk2b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Protein Tyrosine Kinase 2 Beta, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
