Rabbit anti-Human Protein Kinase R Polyclonal Antibody | anti-PKR antibody

Protein Kinase R, Phosphorylated (Thr446) (PKR, ds RNA-dependent Protein Kinase) (HRP)

Cat. Num. AAA6495305

• Gene Names: EIF2AK2; PKR; PRKR; EIF2AK1
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: Protein Kinase R; Polyclonal Antibody; Phosphorylated (Thr446) (PKR; ds RNA-dependent Protein Kinase) (HRP); anti-PKR antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human PKR phosphorylated at Thr446, Mr 68kD. A non-specific protein was also detected, Mr ~75kD, which is inhibited by the immunizing peptide.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-PKR antibody: Western Blot (WB)
• Application Notes: WB: 0.5-2.0ug/ml detects phospho-PKR in RIPA lysates from HeLa cells that had been stimulated with IFN alpha overnight followed by treatment with calyculin A for 15 minutes.; Applications are based on unconjugated antibody.
• Immunogen: Peptide corresponding to amino acids surrounding Thr446 of human PKR.
• Conjugate: HRP
• Antigen Modification: Phosphorylated
• Note: Preservative Free
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Western Blot (WB)

(WB: Treated HeLa (lane 1) or untreated HeLa (lane2) cell lysate was probed with anti-phospho-PKR (0.5ug/ml).)

Related Product Information for anti-PKR antibody

microspheres conjugated to either the phosphorylated or non phosphorylated peptides. Treated HeLa (lane 1) or untreated HeLa (lane2) cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with antiphospho-PKR (0.5ug/ml). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates phospho-PKR (68kD).

Product Categories/Family for anti-PKR antibody

Antibodies; Protein Kinases

References

1. Saelens, X., et al., J. Biol. Chem. 276: 41620, 2001 2. Suen, K.-C., et al., J. Biol. Chem. 2003, e-pub.

NCBI and Uniprot Product Information

• NCBI GI #: 208431827
• NCBI GeneID: 5610
• NCBI Accession #: NP_001129123.1
• NCBI GenBank Nucleotide #: NM_002759
• UniProt Accession #: P19525; Q52M43; Q7Z6F6; Q9UIR4; A8K3P0; D6W584; E9PC80
• Molecular Weight: 62,094 Da
• NCBI Official Full Name: interferon-induced, double-stranded RNA-activated protein kinase isoform a
• NCBI Official Synonym Full Names: eukaryotic translation initiation factor 2-alpha kinase 2
• NCBI Official Symbol: EIF2AK2
• NCBI Official Synonym Symbols: PKR; PRKR; EIF2AK1
• NCBI Protein Information: interferon-induced, double-stranded RNA-activated protein kinase; p68 kinase; eIF-2A protein kinase 2; P1/eIF-2A protein kinase; tyrosine-protein kinase EIF2AK2; interferon-inducible elF2alpha kinase; double stranded RNA activated protein kinase; protein
• UniProt Protein Name: Interferon-induced, double-stranded RNA-activated protein kinase
• Protein Family: Protein kinase
• UniProt Gene Name: EIF2AK2
• UniProt Synonym Gene Names: PKR; PRKR; eIF-2A protein kinase 2; PKR
• UniProt Entry Name: E2AK2_HUMAN

NCBI Description

The protein encoded by this gene is a serine/threonine protein kinase that is activated by autophosphorylation after binding to dsRNA. The activated form of the encoded protein can phosphorylate translation initiation factor EIF2S1, which in turn inhibits protein synthesis. This protein is also activated by manganese ions and heparin. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Oct 2011]

Uniprot Description

PKR: a protein kinase of the PEK family. Upon binding double-stranded RNA, it becomes autophosphorylated and activated. Phosphorylates and inhibits the alpha subunit of eIF2 alpha, which leads to an inhibition of the initiation of protein synthesis. Controls the activation of several transcription factors such as NF-kappaB, p53 and Stats. Mediates apoptosis induced by many different stimuli, such as LPS, TNF-alpha, viral infection and serum starvation.

Protein type: Kinase, protein; Translation; EC 2.7.11.1; Protein kinase, Other; EC 2.7.10.2; Protein kinase, Ser/Thr (non-receptor); Other group; PEK family

Chromosomal Location of Human Ortholog: 2p22-p21

Cellular Component: membrane; perinuclear region of cytoplasm; cytoplasm; ribosome; nucleus; cytosol

Molecular Function: protein serine/threonine kinase activity; protein binding; double-stranded RNA binding; non-membrane spanning protein tyrosine kinase activity; protein phosphatase type 2A regulator activity; ATP binding; eukaryotic translation initiation factor 2alpha kinase activity; protein kinase activity

Biological Process: positive regulation of cytokine production; peptidyl-tyrosine phosphorylation; translation; transcription, DNA-dependent; activation of MAPKK activity; unfolded protein response; response to virus; protein amino acid autophosphorylation; viral infectious cycle; protein amino acid phosphorylation; positive regulation of stress-activated MAPK cascade; positive regulation of chemokine production; evasion by virus of host immune response; activation of NF-kappaB transcription factor; negative regulation of cell proliferation; negative regulation of viral genome replication; modification by virus of host cellular process; virus-host interaction; negative regulation of translation; innate immune response; negative regulation of osteoblast proliferation; defense response to virus; negative regulation of apoptosis