Rabbit anti-Human Protein kinase C epsilon type Polyclonal Antibody | anti-PRKCE antibody

Rabbit anti-human Protein kinase C epsilon type polyclonal Antibody(PRKCE)

Cat. Num. AAA1491958

• Gene Names: PRKCE; PKCE; nPKC-epsilon
• Reactivity: Human
• Applications: ELISA, Immunoprecipitation
• Purity: Antigen Affinity Purified
• Synonyms: Protein kinase C epsilon type; Polyclonal Antibody; Rabbit anti-human Protein kinase C epsilon type polyclonal Antibody(PRKCE); nPKC-epsilon; PRKCE; PKCE; anti-PRKCE antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Antigen Affinity Purified
• Sequence Length: 737

• Applicable Applications for anti-PRKCE antibody: ELISA (EIA), Immunoprecipitation (IP)
• Storage Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
• Conjugate: Non-conjugated
• Immunogen: Recombinant human Protein kinase C epsilon type protein(488-737aa)
• Santa Cruz Alternative: Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-214
• Preparation and Storage: Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C or -80 degree C. Avoid repeated freeze.

Immunoprecipitation (IP)

(Immunoprecipitating PRKCE in MCF-7 whole cell lysateLane 1: Rabbit monoclonal IgG(1ug)instead of MBS1491958 in MCF-7 whole cell lysate.For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the Secondary antibody (1/50000)Lane 2: MBS1491958(4ug)+ MCF-7 whole cell lysate(500ug)Lane 3: MCF-7 whole cell lysate (20ug))

Related Product Information for anti-PRKCE antibody

Calcium-independent, phospholipid- and diacylglycerol (DAG)-dependent serine/threonine-protein kinase that plays essential roles in the regulation of multiple cellular processes linked to cytoskeletal proteins, such as cell adhesion, motility, migration and cell cycle, functions in neuron growth and ion channel regulation, and is involved in immune response, cancer cell invasion and regulation of apoptosis. Mediates cell adhesion to the extracellular matrix via integrin-dependent signaling, by mediating angiotensin-2-induced activation of integrin beta-1 (ITGB1) in cardiac fibroblasts. Phosphorylates MARCKS, which phosphorylates and activates PTK2/FAK, leading to the spread of cardiomyocytes. Involved in the control of the directional transport of ITGB1 in mesenchymal cells by phosphorylating vimentin (VIM), an intermediate filament (IF) protein. In epithelial cells, associates with and phosphorylates keratin-8 (KRT8), which induces targeting of desmoplakin at desmosomes and regulates cell-cell contact. Phosphorylates IQGAP1, which binds to CDC42, mediating epithelial cell-cell detachment prior to migration. In HeLa cells, contributes to hepatocyte growth factor (HGF)-induced cell migration, and in human corneal epithelial cells, plays a critical role in wound healing after activation by HGF. During cytokinesis, forms a complex with YWHAB, which is crucial for daughter cell separation, and facilitates abscission by a mechanism which may implicate the regulation of RHOA. In cardiac myocytes, regulates myofilament function and excitation coupling at the Z-lines, where it is indirectly associated with F-actin via interaction with COPB1. During endothelin-induced cardiomyocyte hypertrophy, mediates activation of PTK2/FAK, which is critical for cardiomyocyte survival and regulation of sarcomere length. Plays a role in the pathogenesis of dilated cardiomyopathy via persistent phosphorylation of troponin I (TNNI3). Involved in nerve growth factor (NFG)-induced neurite outgrowth and neuron morphological change independently of its kinase activity, by inhibition of RHOA pathway, activation of CDC42 and cytoskeletal rearrangement. May be involved in presynaptic facilitation by mediating phorbol ester-induced synaptic potentiation. Phosphorylates gamma-aminobutyric acid receptor subunit gamma-2 (GABRG2), which reduces the response of GABA receptors to ethanol and benzodiazepines and may mediate acute tolerance to the intoxicating effects of ethanol. Upon PMA treatment, phosphorylates the capsaicin- and heat-activated cation channel TRPV1, which is required for bradykinin-induced sensitization of the heat response in nociceptive neurons. Is able to form a complex with PDLIM5 and N-type calcium channel, and may enhance channel activities and potentiates fast synaptic transmission by phosphorylating the pore-forming alpha subunit CACNA1B (CaV2.2). In prostate cancer cells, interacts with and phosphorylates STAT3, which increases DNA-binding and transcriptional activity of STAT3 and seems to be essential for prostate cancer cell invasion. Downstream of TLR4, plays an important role in the lipopolysaccharide (LPS)-induced immune response by phosphorylating and activating TICAM2/TRAM, which in turn activates the transcription factor IRF3 and subsequent cytokines production. In differentiating erythroid progenitors, is regulated by EPO and controls the protection against the TNFSF10/TRAIL-mediated apoptosis, via BCL2. May be involved in the regulation of the insulin-induced phosphorylation and activation of AKT1.

NCBI and Uniprot Product Information

• NCBI GI #: 4885563
• NCBI GeneID: 5581
• NCBI Accession #: NP_005391.1
• NCBI GenBank Nucleotide #: NM_005400.2
• UniProt Accession #: Q02156; Q32MQ3; Q53SL4; Q53SM5; Q9UE81; B0LPH7
• Molecular Weight: 83,674 Da
• NCBI Official Full Name: protein kinase C epsilon type
• NCBI Official Synonym Full Names: protein kinase C epsilon
• NCBI Official Symbol: PRKCE
• NCBI Official Synonym Symbols: PKCE; nPKC-epsilon
• NCBI Protein Information: protein kinase C epsilon type
• UniProt Protein Name: Protein kinase C epsilon type
• Protein Family: Protein kinase
• UniProt Gene Name: PRKCE
• UniProt Synonym Gene Names: PKCE
• UniProt Entry Name: KPCE_HUMAN

NCBI Description

Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by this gene is one of the PKC family members. This kinase has been shown to be involved in many different cellular functions, such as neuron channel activation, apoptosis, cardioprotection from ischemia, heat shock response, as well as insulin exocytosis. Knockout studies in mice suggest that this kinase is important for lipopolysaccharide (LPS)-mediated signaling in activated macrophages and may also play a role in controlling anxiety-like behavior. [provided by RefSeq, Jul 2008]

Uniprot Description

PKCE: an AGC kinase of the PKC family. Calcium-independent, phospholipid-dependent. Activated by inflammatory mediators and involved in nociceptive functions. PKC-epsilon null mice display decreased hypoxic pulmonary vasoconstriction.

Protein type: Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.13; Protein kinase, AGC; Kinase, protein; AGC group; PKC family; Eta subfamily

Chromosomal Location of Human Ortholog: 2p21

Cellular Component: cytoplasm; cytoskeleton; cytosol; endoplasmic reticulum; Golgi apparatus; mitochondrion; nucleus; perinuclear region of cytoplasm; plasma membrane

Molecular Function: actin monomer binding; ATP binding; calcium-independent protein kinase C activity; enzyme activator activity; enzyme binding; ethanol binding; metal ion binding; protein binding; protein kinase C activity; protein serine/threonine kinase activity; receptor activator activity; signal transducer activity

Biological Process: apoptosis; blood coagulation; cell adhesion; cell cycle; cell division; epidermal growth factor receptor signaling pathway; fibroblast growth factor receptor signaling pathway; innate immune response; lipopolysaccharide-mediated signaling pathway; macrophage activation during immune response; negative regulation of protein ubiquitination; nerve growth factor receptor signaling pathway; peptidyl-serine phosphorylation; phospholipase C activation; platelet activation; positive regulation of actin filament polymerization; positive regulation of cytokinesis; positive regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of insulin secretion; positive regulation of lipid catabolic process; positive regulation of MAPKKK cascade; positive regulation of synaptic transmission, GABAergic; protein amino acid phosphorylation; regulation of peptidyl-tyrosine phosphorylation; regulation of release of sequestered calcium ion into cytosol; release of sequestered calcium ion into cytosol; response to morphine; signal transduction