Western Blot (WB)
(Western Blot: Sample: Lane1: Human Hela Cells; Lane2: Mouse Brain Tissue; Lane3: Porcine Brain Tissue.)
Rabbit anti-Human Protein Kinase C Epsilon (PKCe) Polyclonal Antibody | anti-PKCe antibody
Polyclonal Antibody to Protein Kinase C Epsilon (PKCe)
MGHHHHHHSGSEF-LGLDEFNF IKVLGKGSFG KVMLAELKGK DEVYAVKVLK KDVILQDDDV DCTMTEKRIL ALARKHPYLT QLYCCFQTKD RLFFVMEYVN GGDLMFQIQR SRKFDEPRSR FYAAEVTSAL MFLHQHGVIY RDLKLDNILL DAEGHCKLAD FGMCKEGILN GVTTTTFCGT PDYIAPEILQ ELEYGPSVDW WALGVLMYEM MAGQPPFEAD NEDDLFESIL HDDVLYPVWL SKEAVSILKA FMTKNPHKRL GCVASQNGED AIKQHPFFKE ID
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Western Blot (WB)
(Western Blot: Sample: Lane1: Human Hela Cells; Lane2: Mouse Brain Tissue; Lane3: Porcine Brain Tissue.)
Western Blot (WB)
(Western Blot: Sample: Lane1: Human Hela Cells; Lane2: Mouse Brain Tissue; Lane3: Porcine Brain Tissue.)
Western Blot (WB)
(Western Blot: Sample: Recombinant PKCe, Human.)
Western Blot (WB)
(Western Blot: Sample: Recombinant PKCe, Human.)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Human Liver Tissue.)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Human Liver Tissue.)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Human Rectum Tissue)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Human Rectum Tissue)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Human Kidney Tissue.)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Human Kidney Tissue.)
NCBI and Uniprot Product Information
NCBI Description
Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by this gene is one of the PKC family members. This kinase has been shown to be involved in many different cellular functions, such as neuron channel activation, apoptosis, cardioprotection from ischemia, heat shock response, as well as insulin exocytosis. Knockout studies in mice suggest that this kinase is important for lipopolysaccharide (LPS)-mediated signaling in activated macrophages and may also play a role in controlling anxiety-like behavior. [provided by RefSeq, Jul 2008]
Uniprot Description
PKCE: an AGC kinase of the PKC family. Calcium-independent, phospholipid-dependent. Activated by inflammatory mediators and involved in nociceptive functions. PKC-epsilon null mice display decreased hypoxic pulmonary vasoconstriction.
Protein type: AGC group; EC 2.7.11.13; Eta subfamily; Kinase, protein; PKC family; Protein kinase, AGC; Protein kinase, Ser/Thr (non-receptor)
Chromosomal Location of Human Ortholog: 2p21
Cellular Component: cell periphery; cytoplasm; cytoskeleton; cytosol; endoplasmic reticulum; Golgi apparatus; mitochondrion; nucleus; perinuclear region of cytoplasm; plasma membrane
Molecular Function: 14-3-3 protein binding; actin monomer binding; ATP binding; calcium-independent protein kinase C activity; enzyme activator activity; enzyme binding; ethanol binding; metal ion binding; protein binding; protein kinase C activity; protein serine/threonine kinase activity; receptor activator activity; signal transducer activity
Biological Process: apoptosis; cell adhesion; cell cycle; cell division; cellular response to ethanol; cellular response to hypoxia; cellular response to prostaglandin E stimulus; Fc-gamma receptor signaling pathway involved in phagocytosis; lipopolysaccharide-mediated signaling pathway; macrophage activation involved in immune response; negative regulation of protein ubiquitination; negative regulation of sodium ion transmembrane transporter activity; peptidyl-serine phosphorylation; platelet activation; positive regulation of actin filament polymerization; positive regulation of catalytic activity; positive regulation of cell-substrate adhesion; positive regulation of cellular glucuronidation; positive regulation of cytokinesis; positive regulation of epithelial cell migration; positive regulation of fibroblast migration; positive regulation of I-kappaB kinase/NF-kappaB signaling; positive regulation of insulin secretion; positive regulation of lipid catabolic process; positive regulation of MAPK cascade; positive regulation of receptor activity; positive regulation of synaptic transmission, GABAergic; positive regulation of wound healing; protein amino acid phosphorylation; regulation of peptidyl-tyrosine phosphorylation; regulation of release of sequestered calcium ion into cytosol; release of sequestered calcium ion into cytosol; response to morphine; signal transduction
Research Articles on PKCe
Similar Products
Product Notes
The PKCe prkce (Catalog #AAA2002567) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Protein Kinase C Epsilon (PKCe) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Protein Kinase C Epsilon (PKCe) can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB). Western blotting: 1:100-400 Immunocytochemistry in formalin fixed cells: 1:100-500 Immunohistochemistry in formalin fixed frozen section: 1:100-500 Immunohistochemistry in paraffin section: 1:50-200 Enzyme-linked Immunosorbent Assay: 1:100-200. Researchers should empirically determine the suitability of the PKCe prkce for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Antigen: The target protein is fused with N-terminal His-Tag and its sequence is listed below. MGHHHHHHSG SEF-LGLDEF NF IKVLGKGSFG KVMLAELKGK DEVYAVKVLK KDVILQDDDV DCTMTEKRIL ALARKHPYLT QLYCCFQTKD RLFFVMEYVN GGDLMFQIQR SRKFDEPRSR FYAAEVTSAL MFLHQHGVIY RDLKLDNILL DAEGHCKLAD FGMCKEGILN GVTTTTFCGT PDYIAPEILQ ELEYGPSVDW WALGVLMYEM MAGQPPFEAD NEDDLFESIL HDDVLYPVWL SKEAVSILKA FMTKNPHKRL GCVASQNGED AIKQHPFFKE ID. It is sometimes possible for the material contained within the vial of "Protein Kinase C Epsilon (PKCe), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
