Rabbit anti-Human Protein Kinase A, beta, Catalytic Subunit Polyclonal Antibody | anti-PRKACB antibody

Protein Kinase A, beta, Catalytic Subunit, phosphorylated (Ser338) (PKAb)

Cat. Num. AAA626941

• Gene Names: PRKACB; PKACB; MGC9320; MGC41879; DKFZp781I2452
• Reactivity: Human
• Applications: Western Blot
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: Protein Kinase A; beta; Catalytic Subunit; Polyclonal Antibody; phosphorylated (Ser338) (PKAb); Anti -Protein Kinase A; anti-PRKACB antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human PKA catalytic b subunit when phosphorylated at Ser338. Species sequence homology: bovine and porcine. Peptide competition data indicate that this antibody cross-reacts with the PKA y subunit (64% homologous) and partially with the a subunit (82% homologous).
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS (without Mg2+ and Ca2+), 1mg/ml BSA (IgG, protease free), pH 7.2, 0.05% sodium azide, 50% glycerol.

• Applicable Applications for anti-PRKACB antibody: ChlP, Western Blot (WB)
• Application Notes: Suitable for use in Western blotting applications.; Dilution:; ChlP: 1:100; Western Blot: 1:1000.; Other applications not tested.
• Immunogen: Synthetic phosphopeptide derived from a region of human PKA catalytic b subunit that contains serine 338.; Species sequence homology: bovine and porcine.
• Positive Control: 3T3-L1 adipocytes
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Related Product Information for anti-PRKACB antibody

c-AMP-dependent Protein Kinase (PKA) is a serine/threonine kinase that regulates a number of cellular processes including proliferation, ion transport and gene transcription. PKA is composed of conserved catalytic subunits and regulatory subunits that dissociate upon activation by cAMP. The catalytic subunit of PKA contains the activation loop and mediates DNA binding and substrate recognition. The catalytic subunit is assembled and expressed as an active form and is phosphorylated on threonine 197 by PDK-1 in the activation loop and serine 338 in the carboxyl terminus. Phosphorylation of serine 338 plays a key role in stabilizing PKA and activating its substrates and hence mediating its biological functions.

Product Categories/Family for anti-PRKACB antibody

Antibodies; Abs to Protein Kinases

NCBI and Uniprot Product Information

• NCBI GI #: 23268606
• NCBI GeneID: 5567
• UniProt Accession #: P22694; Q14VH1; Q59GC0; Q5BNE9; Q5BNF0; Q5BNF1; Q5BNF2; Q5BNF3; Q5CZ92; Q5T1K3; Q7Z3M1; B4E2Q1
• NCBI Official Full Name: protein kinase A catalytic subunit beta
• NCBI Official Synonym Full Names: protein kinase, cAMP-dependent, catalytic, beta
• NCBI Official Symbol: PRKACB
• NCBI Official Synonym Symbols: PKACB; MGC9320; MGC41879; DKFZp781I2452
• NCBI Protein Information: cAMP-dependent protein kinase catalytic subunit beta; PKA C-beta; OTTHUMP00000011663; OTTHUMP00000011664; OTTHUMP00000011666; OTTHUMP00000011667; OTTHUMP00000011669; OTTHUMP00000011671; OTTHUMP00000011673; OTTHUMP00000011674; protein kinase A catalytic subunit beta; cAMP-dependent protein kinase catalytic beta subunit isoform 4ab
• UniProt Protein Name: cAMP-dependent protein kinase catalytic subunit beta
• Protein Family: cAMP-dependent protein kinase
• UniProt Gene Name: PRKACB
• UniProt Entry Name: KAPCB_HUMAN

NCBI Description

cAMP is a signaling molecule important for a variety of cellular functions. cAMP exerts its effects by activating the cAMP-dependent protein kinase, which transduces the signal through phosphorylation of different target proteins. The inactive kinase holoenzyme is a tetramer composed of two regulatory and two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits have been identified in humans. The protein encoded by this gene is a member of the Ser/Thr protein kinase family and is a catalytic subunit of cAMP-dependent protein kinase. Several alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq]

Uniprot Description

PKACB: Mediates cAMP-dependent signaling triggered by receptor binding to GPCRs. PKA activation regulates diverse cellular processes such as cell proliferation, the cell cycle, differentiation and regulation of microtubule dynamics, chromatin condensation and decondensation, nuclear envelope disassembly and reassembly, as well as regulation of intracellular transport mechanisms and ion flux. Regulates the abundance of compartmentalized pools of its regulatory subunits through phosphorylation of PJA2 which binds and ubiquitinates these subunits, leading to their subsequent proteolysis. A number of inactive tetrameric holoenzymes are produced by the combination of homo- or heterodimers of the different regulatory subunits associated with two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. The cAMP-dependent protein kinase catalytic subunit binds PJA2. Isoform 1 is most abundant in the brain, with low level expression in kidney. Isoform 2 is predominantly expressed in thymus, spleen and kidney. Isoform 3 and isoform 4 are only expressed in the brain. Activated by cAMP. Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. cAMP subfamily. 9 isoforms of the human protein are produced by alternative splicing.

Protein type: Protein kinase, Ser/Thr (non-receptor); Protein kinase, AGC; EC 2.7.11.11; Kinase, protein; AGC group; PKA family

Chromosomal Location of Human Ortholog: 1p31.1

Cellular Component: nucleoplasm; centrosome; perinuclear region of cytoplasm; plasma membrane; cytosol; cAMP-dependent protein kinase complex

Molecular Function: protein binding; cAMP-dependent protein kinase activity; ubiquitin protein ligase binding; magnesium ion binding; ATP binding

Biological Process: epidermal growth factor receptor signaling pathway; fibroblast growth factor receptor signaling pathway; nerve growth factor receptor signaling pathway; water transport; activation of protein kinase A; glucose metabolic process; pathogenesis; negative regulation of meiotic cell cycle; signal transduction; protein amino acid phosphorylation; gluconeogenesis; G-protein signaling, coupled to cAMP nucleotide second messenger; synaptic transmission; phospholipase C activation; neural tube closure; carbohydrate metabolic process; triacylglycerol catabolic process; energy reserve metabolic process; innate immune response; renal water homeostasis; blood coagulation; transmembrane transport; regulation of insulin secretion