Protein G Polyclonal Antibody
Protein G-UNLB
Applications
Immunoprecipitation
Synonyms
Protein G; Polyclonal Antibody; Protein G-UNLB; Protein G - Purified; Unlabeled Antibody; anti-Protein G antibody
Clonality
Polyclonal
Specificity
Protein G binds the Fc portion of IgGs from many species including all human, and mouse (IgG1, weakly) subclasses. It does not bind IgM, IgA, IgE or serum albumin.
Form/Format
Purified (UNLB) Protein G
Applicable Applications for anti-Protein G antibody
Immunoprecipitation
Application Notes
Research Application(s): Gamma immunoglobulin capture
Conjugation on solid phase or others to detect gamma immunoglobulin
Conjugation on solid phase or others to detect gamma immunoglobulin
Source
Recombinant, from Streptococcus Strain G148, produced in E Coli.
Mr/Structure
22 KD. Protein G is a monomeric protein lacking cystine residues; on SDS gel, it migrates to 32KD.
Characterization
To insure lot-to-lot consistency, each batch of product is tested by FPLC and ELISA to conform to the characteristics of a standard reference reagent.
Preparation and Storage
The purified Protein G is supplied as either 1.0 mg or 5.0 mg in lyophilized form. No BSA, preservatives or amine-containing buffer salts have been added. Store up to one year at -20 degree C; or after reconstitution in phosphate buffered saline to highest concentration possible, store up to one month at 2-8 degree C.
Protein G-HRP conjugate is supplied as 1.0 mL of stock solution in 50% glycerol/50% PBS, pH 7.4. No other preservatives have been added. Store at 2-8 degree C or long-term at -20 degree C.
Reagents are stable for the period shown on the label if stored as directed.
Protein G-HRP conjugate is supplied as 1.0 mL of stock solution in 50% glycerol/50% PBS, pH 7.4. No other preservatives have been added. Store at 2-8 degree C or long-term at -20 degree C.
Reagents are stable for the period shown on the label if stored as directed.
References
1. Saegerman, C., L. De Waele, D. Gilson, J. Godfroid, P. Thiange, P. Michel, B. Limbourg, T.K. Vo, J. Limet, J.J. Letesson, and D. Berkvens. 2004. Vet. Microbiol. 100:91.
2. Qi, Y., Z. Yan, and J. Huang. 2001. J. Biochem. Biophys. Methods 49:263.
3. Sugiura, T., H. Imahawa, and T. Kondo. 2000. J. Chromatogr. B. Biomed. Sci. Appl. 742:327.
4. Yan. Z., and J. Huang. 2000. J. Immunol. Methods 237:203.
5. Aybay, C., and T. Imir. 2000. J. Immunol. Methods 233:77.
6. Bastisa-Corcuera, F.D., K.H. Nielsen, and L.B. Corbeil. 1999. Vet. Immunol. Immunopathol. 71:143.
7. And, Q., G. Luo, J. Ou, and W.S. Yeung. 1999. J. Chromatogr. A. 848:139.
8. Goward CR, Murphy JP, Atkinson T, Barstow DA Expression and purification of a truncated recombinant streptococcal protein G. Biochem J. 1990 Apr 1;267(1):171-7.
9. Guss B, Eliasson M, Olsson A, Uhlén M, Frej AK, Jornvall H, Flock JI, Lindberg M. Structure of the IgG-binding regions of streptococcal protein G. EMBO J. 1986 Jul;5(7):1567-75.
2. Qi, Y., Z. Yan, and J. Huang. 2001. J. Biochem. Biophys. Methods 49:263.
3. Sugiura, T., H. Imahawa, and T. Kondo. 2000. J. Chromatogr. B. Biomed. Sci. Appl. 742:327.
4. Yan. Z., and J. Huang. 2000. J. Immunol. Methods 237:203.
5. Aybay, C., and T. Imir. 2000. J. Immunol. Methods 233:77.
6. Bastisa-Corcuera, F.D., K.H. Nielsen, and L.B. Corbeil. 1999. Vet. Immunol. Immunopathol. 71:143.
7. And, Q., G. Luo, J. Ou, and W.S. Yeung. 1999. J. Chromatogr. A. 848:139.
8. Goward CR, Murphy JP, Atkinson T, Barstow DA Expression and purification of a truncated recombinant streptococcal protein G. Biochem J. 1990 Apr 1;267(1):171-7.
9. Guss B, Eliasson M, Olsson A, Uhlén M, Frej AK, Jornvall H, Flock JI, Lindberg M. Structure of the IgG-binding regions of streptococcal protein G. EMBO J. 1986 Jul;5(7):1567-75.
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Product Notes
The Protein G (Catalog #AAA675014) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Protein G can be used in a range of immunoassay formats including, but not limited to, Immunoprecipitation. Research Application(s): Gamma immunoglobulin capture Conjugation on solid phase or others to detect gamma immunoglobulin. Researchers should empirically determine the suitability of the Protein G for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Protein G, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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