Rabbit anti-Human Prostate Speci?c Antigen Polyclonal Antibody | anti-PSCA antibody

Prostate Speci?c Antigen (PSA, Kallikrein-3, Kallikrein III, Seminin, Semenogelase, gamma-Seminoprotein, P-30 antigen, KLK3)

Cat. Num. AAA646847

• Gene Names: PSCA; PRO232
• Reactivity: Human
• Applications: Western Blot
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: Prostate Speci?c Antigen; Polyclonal Antibody; Prostate Speci?c Antigen (PSA; Kallikrein-3; Kallikrein III; Seminin; Semenogelase; gamma-Seminoprotein; P-30 antigen; KLK3); Anti -Prostate Speci c Antigen (PSA; -Seminoprotein; anti-PSCA antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Reacts with residues 247-261 [VHYRKWIKDTIVANP] of the human KLK3 protein.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2, 0.01% sodium azide before the addition of 40% glycerol.

• Applicable Applications for anti-PSCA antibody: Western Blot (WB)
• Application Notes: Suitable for use in Western Blot.; Dilution: Western Blot: 1:250-1:500
• Immunogen: Synthetic peptide, aa247-261 [VHYRKWIKDTIVANP] of the human KLK3 protein.
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for anti-PSCA antibody

Kallikreins are a subgroup of serine proteases and are implicated in carcinogenesis. KLK 3 (also known as prostate speci c antigen) is a marker for prostatic cancer and is involved in the pathogenesis and/or progression of prostate, breast, and possibly other malignancies.

Product Categories/Family for anti-PSCA antibody

Antibodies; Abs to Kallikrein

NCBI and Uniprot Product Information

• NCBI GI #: 289547757
• NCBI GeneID: 8000
• NCBI Accession #: NP_005663.2
• NCBI GenBank Nucleotide #: NM_005672.4
• UniProt Accession #: O43653; Q6UW92
• Molecular Weight: 12,912 Da
• NCBI Official Full Name: prostate stem cell antigen preproprotein
• NCBI Official Synonym Full Names: prostate stem cell antigen
• NCBI Official Symbol: PSCA
• NCBI Official Synonym Symbols: PRO232
• NCBI Protein Information: prostate stem cell antigen
• UniProt Protein Name: Prostate stem cell antigen
• Protein Family: Prostate stem cell antigen
• UniProt Gene Name: PSCA
• UniProt Entry Name: PSCA_HUMAN

NCBI Description

This gene encodes a glycosylphosphatidylinositol-anchored cell membrane glycoprotein. In addition to being highly expressed in the prostate it is also expressed in the bladder, placenta, colon, kidney, and stomach. This gene is up-regulated in a large proportion of prostate cancers and is also detected in cancers of the bladder and pancreas. This gene includes a polymorphism that results in an upstream start codon in some individuals; this polymorphism is thought to be associated with a risk for certain gastric and bladder cancers. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2010]

Uniprot Description

Function: May be involved in the regulation of cell proliferation. Has a cell-proliferation inhibition activity in vitro. Ref.8

Subcellular location: Cell membrane; Lipid-anchor › GPI-anchor.

Tissue specificity: Highly expressed in prostate (basal, secretory and neuroendocrine epithelium cells). Also found in bladder (transitional epithelium), placenta (trophoblasts), stomach (neuroendocrine cells), colon (neuroendocrine cells) and kidney (collecting ducts). Overexpressed in prostate cancers and expression is correlated with tumor stage, grade and androgen-independence. Highly expressed in prostate cancer bone metastases. Expressed in gastric epithelial cells, mainly in the isthmus (at protein level). Not detected in normal intestinal epithelium (at protein level). Ref.7 Ref.8

Induction: Down-regulated in gastric cancer cells. Ref.8

Polymorphism: Genetic variations in PSCA may influence susceptibility to diffuse-type gastric cancer.

Sequence similarities: Contains 1 UPAR/Ly6 domain.

Caution: The start codon presents some polymorphism and the initiation methionine is a threonine on the genome.