Rabbit anti-Human, Mouse Proprotein Convertase 2 Polyclonal Antibody | anti-PC2 antibody

Proprotein Convertase 2, NT (PC2, KEX2 Like Endoprotease 2, KEX2-like Endoprotease 2, Neuroendocrine Convertase 2, NEC 2, NEC2, Prohormone Convertase 2, Proprotein Convertase Subtilisin/kexin Type 2, PC2, PCSK2, SPC2) (MaxLight 405)

Cat. Num. AAA6494704

• Gene Names: PCSK2; PC2; NEC2; SPC2; NEC 2; NEC-2
• Reactivity: Human, Mouse
• Applications: Western Blot
• Purity: Purified by Ammonium Sulfate Precipitation.
• Synonyms: Proprotein Convertase 2; Polyclonal Antibody; NT (PC2; KEX2 Like Endoprotease 2; KEX2-like Endoprotease 2; Neuroendocrine Convertase 2; NEC 2; NEC2; Prohormone Convertase 2; Proprotein Convertase Subtilisin/kexin Type 2; PC2; PCSK2; SPC2) (MaxLight 405); EC=3.4.21.94; NEC-2; anti-PC2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human PCSK2. Species Crossreactivity: mouse.
• Purity/Purification: Purified by Ammonium Sulfate Precipitation.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight405.

• Applicable Applications for anti-PC2 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:50-1:100; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the N-terminal region of human PCSK2 (KLH).
• Conjugate: MaxLight405
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight405 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of PCSK2 Antibody (N-term) in mouse cerebellum tissue lysates (35ug/lane). PCSK2 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human brain tissue reacted with PCSK2 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Immunofluorescence (IF)

(Confocal immunofluorescent analysis of PCSK2 Antibody (N-term) with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)

Flow Cytometry (FC/FACS)

(PCSK2 Antibody (N-term) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Product Categories/Family for anti-PC2 antibody

Antibodies; Hormones; Steroids

References

1. Leak, T.S., Keene, K.L. Mol. Genet. Metab. 92(1-2), 145-150 (2007). 2. Shen, X., Li, Q.L. Am. J. Physiol. Endocrinol. Metab. 288(1), E236-E245 (2005). 3. Tzimas, G.N., Chevet, E. BMC Cancer 5, 149 (2005). 4. Seidah, N.G., Mattei, M.G. Genomics 11(1), 103-107 (1991).

NCBI and Uniprot Product Information

• NCBI GI #: 20336244
• NCBI GeneID: 5126
• NCBI Accession #: NP_002585
• NCBI GenBank Nucleotide #: NM_002594
• UniProt Accession #: P16519
• Molecular Weight: 58kDa
• NCBI Official Full Name: neuroendocrine convertase 2 isoform 1 preproprotein
• NCBI Official Synonym Full Names: proprotein convertase subtilisin/kexin type 2
• NCBI Official Symbol: PCSK2
• NCBI Official Synonym Symbols: PC2; NEC2; SPC2; NEC 2; NEC-2
• NCBI Protein Information: neuroendocrine convertase 2
• UniProt Protein Name: Neuroendocrine convertase 2
• Protein Family: Putative envelope glycoprotein
• UniProt Gene Name: PCSK2
• UniProt Synonym Gene Names: NEC2; NEC 2; PC2
• UniProt Entry Name: NEC2_HUMAN

NCBI Description

This gene encodes a member of the subtilisin-like proprotein convertase family, which includes proteases that process protein and peptide precursors trafficking through regulated or constitutive branches of the secretory pathway. The protein undergoes an initial autocatalytic processing event and interacts with a neuroendocrine secretory protein in the ER, exits the ER and sorts to secretory granules, where it is cleaved and catalytically activated during intracellular transport. The encoded protease is packaged into and activated in dense core secretory granules and expressed in the neuroendocrine system and brain. This gene encodes one of the seven basic amino acid-specific members which cleave their substrates at single or paired basic residues. It functions in the proteolytic activation of polypeptide hormones and neuropeptides precursors. Single nucleotide polymorphisms in this gene may increase susceptibility to myocardial infarction and type 2 diabetes. This gene may also play a role in tumor development and progression. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Jan 2014]

Uniprot Description

PCSK2: Involved in the processing of hormone and other protein precursors at sites comprised of pairs of basic amino acid residues. Belongs to the peptidase S8 family. Furin subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Protease; EC 3.4.21.94

Chromosomal Location of Human Ortholog: 20p11.2

Cellular Component: extracellular space; transport vesicle; membrane; dendrite; perikaryon

Molecular Function: protein binding; serine-type endopeptidase activity; protein complex binding

Biological Process: insulin processing; nervous system development; protein autoprocessing; cellular protein metabolic process; proteolysis