Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Rabbit anti-Rat Prolyl-4-Hydroxylase Alpha Polypeptide I Polyclonal Antibody | anti-P4Ha1 antibody
Polyclonal Antibody to Prolyl-4-Hydroxylase Alpha Polypeptide I (P4Ha1)
Gene Names
P4ha1; PHalphaI
Reactivity
Rat
Applications
Western Blot, Immunocytochemistry, Immunohistochemistry, ELISA
Purity
Affinity Chromatography
Synonyms
Prolyl-4-Hydroxylase Alpha Polypeptide I; Polyclonal Antibody; Polyclonal Antibody to Prolyl-4-Hydroxylase Alpha Polypeptide I (P4Ha1); anti-P4Ha1 antibody
Host
Rabbit
Reactivity
Rat
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against P4Ha1. It has beenselected for its ability to recognize P4Ha1 in immunohistochemical staining andwestern blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Liquid
Concentration
500ug/mL (varies by lot)
Applicable Applications for anti-P4Ha1 antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA)
Application Notes
Western blotting: 0.2-2ug/mL;1:250-2500
Immunohistochemistry: 5-20ug/mL;1:25-100
Immunocytochemistry: 5-20ug/mL;1:25-100
Optimal working dilutions must be determined by end user.
Immunohistochemistry: 5-20ug/mL;1:25-100
Immunocytochemistry: 5-20ug/mL;1:25-100
Optimal working dilutions must be determined by end user.
Fragment
P4Ha1 (Val205~Glu534)
Organism Species
Rattus norvegicus (Rat)
Cross Reactivity
Rat
Conjugate
No Conjugate
Immunogen
Recombinant P4Ha1 (Val205~Glu534) expressed in E Coli.
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2074277
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Immunohistochemistry (IHC)
(DABstainingonIHC-P.Samples:RatTissue))
Immunohistochemistry (IHC)
(DABstainingonIHC-P.Samples:RatTissue))
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
60,898 Da
NCBI Official Full Name
prolyl 4-hydroxylase subunit alpha-1
NCBI Official Synonym Full Names
prolyl 4-hydroxylase subunit alpha 1
NCBI Official Symbol
P4ha1
NCBI Official Synonym Symbols
PHalphaI
NCBI Protein Information
prolyl 4-hydroxylase subunit alpha-1
UniProt Protein Name
Prolyl 4-hydroxylase subunit alpha-1
Protein Family
UniProt Gene Name
P4ha1
UniProt Synonym Gene Names
P4ha; 4-PH alpha-1
NCBI Description
catalyzes the hydroxylation of -X-Pro-Gly- triplets to form hydroxyproline [RGD, Feb 2006]
Uniprot Description
Catalyzes the post-translational formation of 4-hydroxyproline in -Xaa-Pro-Gly- sequences in collagens and other proteins.
Similar Products
Product Notes
The P4Ha1 p4ha1 (Catalog #AAA2026231) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Prolyl-4-Hydroxylase Alpha Polypeptide I (P4Ha1) reacts with Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Prolyl-4-Hydroxylase Alpha Polypeptide I can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA). Western blotting: 0.2-2ug/mL;1:250-2500 Immunohistochemistry: 5-20ug/mL;1:25-100 Immunocytochemistry: 5-20ug/mL;1:25-100 Optimal working dilutions must be determined by end user. . Researchers should empirically determine the suitability of the P4Ha1 p4ha1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Prolyl-4-Hydroxylase Alpha Polypeptide I, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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