Rabbit anti-Human Prohibitin Polyclonal Antibody | anti-PHB antibody

Prohibitin, NT (PHB) (FITC)

Cat. Num. AAA6494548

• Gene Names: PHB; PHB1; HEL-215; HEL-S-54e
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Immunoaffinity chromatography.
• Synonyms: Prohibitin; Polyclonal Antibody; NT (PHB) (FITC); anti-PHB antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human PHB1.
• Purity/Purification: Purified by Immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-PHB antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:50-1:100; FLISA: 1:1,000; WB: 1:50-1:100; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the N-terminal region of human PHB1 (KLH).
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(PHB1 Antibody (Human N-term) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the PHB1 antibody detected the PHB1 protein (arrow).)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human hepatocarcinoma with Prohibitin (PHB1) Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(Flow cytometric analysis of ATDC5 cells using SLC22A23 Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Product Categories/Family for anti-PHB antibody

Antibodies; Membrane Proteins

References

1. Gregory-Bass, R.C., Int. J. Cancer 122(9), 1923-1930 (2008). 2. Ross, J.A., J. Biol. Chem. 283(8), 4699-4713 (2008). 3. White, J.J., Genomics 11(1), 228-230 (1991).

NCBI and Uniprot Product Information

• NCBI GI #: 4505773
• NCBI GeneID: 5245
• NCBI Accession #: NP_002625
• NCBI GenBank Nucleotide #: NM_002634.3
• UniProt Accession #: P35232; Q4VBQ0; B4DY47
• Molecular Weight: 31.9 kDa (292aa) confirmed by MALDI-TOF
• NCBI Official Full Name: prohibitin isoform 1
• NCBI Official Synonym Full Names: prohibitin
• NCBI Official Symbol: PHB
• NCBI Official Synonym Symbols: PHB1; HEL-215; HEL-S-54e
• NCBI Protein Information: prohibitin
• UniProt Protein Name: Prohibitin
• Protein Family: Prohibitin
• UniProt Gene Name: PHB
• UniProt Entry Name: PHB_HUMAN

NCBI Description

This gene is evolutionarily conserved, and its product is proposed to play a role in human cellular senescence and tumor suppression. Antiproliferative activity is reported to be localized to the 3' UTR, which is proposed to function as a trans-acting regulatory RNA. Several pseudogenes of this gene have been identified. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013]

Uniprot Description

PHB: a pleiotropic membrane protein that suppresses cell proliferation, apoptosis and senescence. It plays a role both in maintaining mitochondrial integrity and in cell cycle regulation. Expression increases approximately 3-fold upon entry into G1 phase compared with other phases of the cell cycle. It helps prevent ROS-induced senescence and its expression decreases heterogeneously during cellular aging. Helps maintain the angiogenic capacity of endothelial cells. Up-regulated during activation of primary human T cells via CD3/CD28 pathways. Present in multiple cellular compartments. Large assemblies of PHB and PHB2 localize in the inner membrane of mitochondria. Acts as a mitochondrial chaperone protein, regulates mitochondrial morphogenesis, and helps regulate the organization of mitochondrial DNA. Reported to target lipid rafts. In the nucleus, it has been reported to interact with various transcription factors, modulating their activity. A potential tumor suppressor that functions as a potent transcriptional corepressor for estrogen receptor alpha. It is downregulated by androgens, and represses androgen receptor activity. Co-localizes with Rb in the nucleus and recruits N-CoR and HDAC1 for transcriptional repression. In vivo promoter occupancy studies have suggested that the PHB gene is a direct target of c-Myc. May bind to and enhance the transcriptional activity of p53. Both Phb and Phb2 are present in the circulation and can be internalized by cultured cells. Overexpressed in endometrioid ovarian adenocarcinoma and papillary serous ovarian carcinoma. Mutated in sporadic breast cancer.

Protein type: Transcription, coactivator/corepressor; Apoptosis; Oncoprotein; Mitochondrial; Chaperone; Nuclear receptor co-regulator; Motility/polarity/chemotaxis; Cell cycle regulation

Chromosomal Location of Human Ortholog: 17q21

Cellular Component: nucleoplasm; membrane; mitochondrion; integral to plasma membrane; mitochondrial inner membrane; cytoplasm; nucleus

Molecular Function: protein binding; enzyme binding; histone deacetylase binding

Biological Process: transcription from RNA polymerase II promoter; osteoblast differentiation; regulation of apoptosis; negative regulation of cell proliferation; progesterone receptor signaling pathway; regulation of transcription, DNA-dependent; positive regulation of transcription, DNA-dependent; histone deacetylation; negative regulation of cell growth; negative regulation of transcription from RNA polymerase II promoter; signal transduction; negative regulation of transcription, DNA-dependent

Disease: Breast Cancer