Rabbit PRNP/PrP/Prion Polyclonal Antibody | anti-PRNP antibody

Anti-PRNP/PrP/Prion Antibody (clone T16-R)

Cat. Num. AAA2400911

• Gene Names: PRNP; CJD; GSS; PrP; ASCR; KURU; PRIP; PrPc; CD230; AltPrP; p27-30; PrP27-30; PrP33-35C
• Reactivity: Human; Predicted: Mouse, Sheep, Bovine, Rat
• Applications: ELISA, Immunohistochemistry, Immunoprecipitation, Western Blot
• Purity: Affinity Purified
• Synonyms: PRNP/PrP/Prion; Polyclonal Antibody; Anti-PRNP/PrP/Prion Antibody (clone T16-R); Mouse Monoclonal [clone EM-20] (IgG2a) to Human PRNP/PrP/Prion; PRNP; AltPrP; ASCR; CD230 antigen; CJD; KURU; Major prion protein; p27-30; Prion; Prion-related protein; PRIP; Prion protein (p27-30); PrP; PrP33-35C; BSE; CD230; Prion protein; PrP27-30; PrPc; anti-PRNP antibody

• Host: Rabbit
• Reactivity: Human; Predicted: Mouse, Sheep, Bovine, Rat
• Clonality: Polyclonal
• Clone Number: T16-R
• Specificity: Antibody recognizes both forms of prion protein - PrPC and PrPSC, untreated with proteinase-K.
• Purity/Purification: Affinity Purified
• Form/Format: 20 mM Tris-HCl, pH 8.0, 10 mg/ml BSA stabilzer, 0.05% sodium azide preservative
• Sequence Length: 253

• Applicable Applications for anti-PRNP antibody: ELISA (EIA), Immunohistochemistry (IHC) Paraffin, Immunoprecipitation (IP), Western Blot (WB)
• Application Notes: ELISA: 1:10000-1:20000; IHC-P: 1:100; WB: 1:1000
• Immunogen: Peptide derived from N-terminal peptide of human prion protein, just before the first octapeptide sequnce repeat
• Conjugation: Unconjugated
• Preparation and Storage: Aliquot and store at -20 degree C. Minimize freezing and thawing.

Immunohistochemistry (IHC)

(Anti-PRNP/PrP/Prion antibody IHC staining of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)

Related Product Information for anti-PRNP antibody

PRNP/PrP/Prion is a membrane glycosylphosphatidylinositol-anchoredglycoprotein that tends to aggregate into rod-like structures. the encoded protein contains a highly unstable region of five tandemoctapeptide repeats. This gene is found on chromosome 20, approximately 20 kbp upstream of a gene which encodes a biochemically and structurally similar protein to the one encoded by this gene.

NCBI and Uniprot Product Information

• NCBI GI #: 4506113
• NCBI GeneID: 5621
• NCBI Accession #: NP_000302.1
• NCBI GenBank Nucleotide #: NM_000311.4
• UniProt Accession #: P04156; O60489; P78446; Q15216; Q15221; Q27H91; Q5QPB4; Q8TBG0; Q96E70; Q9UP19; F7VJQ1
• Molecular Weight: 8,691 Da
• NCBI Official Full Name: major prion protein Prp preproprotein
• NCBI Official Synonym Full Names: prion protein
• NCBI Official Symbol: PRNP
• NCBI Official Synonym Symbols: CJD; GSS; PrP; ASCR; KURU; PRIP; PrPc; CD230; AltPrP; p27-30; PrP27-30; PrP33-35C
• NCBI Protein Information: major prion protein; alternative prion protein
• UniProt Protein Name: Major prion protein
• Protein Family: Major prion protein
• UniProt Gene Name: PRNP
• UniProt Synonym Gene Names: ALTPRP; PRIP; PRP; PrP

NCBI Description

The protein encoded by this gene is a membrane glycosylphosphatidylinositol-anchored glycoprotein that tends to aggregate into rod-like structures. The encoded protein contains a highly unstable region of five tandem octapeptide repeats. This gene is found on chromosome 20, approximately 20 kbp upstream of a gene which encodes a biochemically and structurally similar protein to the one encoded by this gene. Mutations in the repeat region as well as elsewhere in this gene have been associated with Creutzfeldt-Jakob disease, fatal familial insomnia, Gerstmann-Straussler disease, Huntington disease-like 1, and kuru. An overlapping open reading frame has been found for this gene that encodes a smaller, structurally unrelated protein, AltPrp. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2014]

Uniprot Description

Its primary physiological function is unclear. May play a role in neuronal development and synaptic plasticity. May be required for neuronal myelin sheath maintenance. May promote myelin homeostasis through acting as an agonist for ADGRG6 receptor. May play a role in iron uptake and iron homeostasis. Soluble oligomers are toxic to cultured neuroblastoma cells and induce apoptosis (in vitro) (). Association with GPC1 (via its heparan sulfate chains) targets PRNP to lipid rafts. Also provides Cu2+ or ZN2+ for the ascorbate-mediated GPC1 deaminase degradation of its heparan sulfate side chains ().