Rabbit anti-Human PRMT7 Polyclonal Antibody | anti-PRMT7 antibody

PRMT7, CT (Protein Arginine N-methyltransferase 7, PRMT-7, Histone-arginine N-methyltransferase PRMT7, [Myelin Basic Protein]-arginine N-methyltransferase PRMT7, KIAA1933) (HRP)

Cat. Num. AAA6494483

• Gene Names: PRMT7; SBIDDS
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Ammonium Sulfate Precipitation.
• Synonyms: PRMT7; Polyclonal Antibody; CT (Protein Arginine N-methyltransferase 7; PRMT-7; Histone-arginine N-methyltransferase PRMT7; [Myelin Basic Protein]-arginine N-methyltransferase PRMT7; KIAA1933) (HRP); anti-PRMT7 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human PRMT7.
• Purity/Purification: Purified by Ammonium Sulfate Precipitation.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-PRMT7 antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:50-1:100; ELISA: 1:1,000; WB: 1:100-1:500; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide in the C-term region of human PRMT7 (KLH).
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.

Western Blot (WB)

(Western blot analysis of PRMT7 (arrow) using rabbit polyclonal PRMT7 Antibody (C-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PRMT7 gene (Lane 2) (Origene Technologies).)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

Product Categories/Family for anti-PRMT7 antibody

Antibodies; Enzymes; Methyltransferase

References

1. Lee, J.H., et al. J. Biol. Chem. 280(5), 3656-3664 (2005). 2. Miranda, T.B., et al. J. Biol. Chem. 279(22), 22902-22907 (2004). 3. Frankel A., et al. J. Biol. Chem. 277:3537-3543(2002). 4. Pal, S., et al., Mol. Cell. Biol. 23(21):7475-7487 (2003). 5. Rho, J., et al., J. Biol. Chem. 276(14):11393-11401 (2001). 6.Pollack, B.P., et al., J. Biol. Chem. 274(44):31531-31542 (1999). 7.Gilbreth, M., et al., PNAS 95(25):14781-14786 (1998).

NCBI and Uniprot Product Information

• NCBI GI #: 9506615
• NCBI GeneID: 54496
• NCBI Accession #: NP_061896
• NCBI GenBank Nucleotide #: NM_019023
• UniProt Accession #: Q9NVM4
• Molecular Weight: 78kDa
• NCBI Official Full Name: protein arginine N-methyltransferase 7 isoform 1
• NCBI Official Synonym Full Names: protein arginine methyltransferase 7
• NCBI Official Symbol: PRMT7
• NCBI Official Synonym Symbols: SBIDDS
• NCBI Protein Information: protein arginine N-methyltransferase 7
• UniProt Protein Name: Protein arginine N-methyltransferase 7
• UniProt Gene Name: PRMT7
• UniProt Synonym Gene Names: KIAA1933
• UniProt Entry Name: ANM7_HUMAN

NCBI Description

This gene encodes a member of the protein arginine N-methyltransferase family of proteins. The encoded enzyme transfers single methyl groups to arginine residues to generate monomethylarginines on histone proteins as well as other protein substrates. This enzyme plays a role in a wide range of biological processes, including neuronal differentiation, male germ line imprinting, small nuclear ribonucleoprotein biogenesis, and regulation of the Wnt signaling pathway. Mutations in this gene underlie multiple related syndromes in human patients characterized by intellectual disability, short stature and other features. The encoded protein may promote breast cancer cell invasion and metastasis in human patients. [provided by RefSeq, May 2017]

Uniprot Description

PRMT7: Arginine methyltransferase that can both catalyze the formation of omega-N monomethylarginine (MMA) and symmetrical dimethylarginine (sDMA), with a preference for the formation of MMA. Specifically mediates the symmetrical dimethylation of arginine residues in the small nuclear ribonucleoproteins Sm D1 (SNRPD1) and Sm D3 (SNRPD3); such methylation being required for the assembly and biogenesis of snRNP core particles. Specifically mediates the symmetric dimethylation of histone H4 'Arg-3' to form H4R3me2s. Plays a role in gene imprinting by being recruited by CTCFL at the H19 imprinted control region (ICR) and methylating histone H4 to form H4R3me2s, possibly leading to recruit DNA methyltransferases at these sites. May also play a role in embryonic stem cell (ESC) pluripotency. Also able to mediate the arginine methylation of histone H2A and myelin basic protein (MBP) in vitro; the relevance of such results is however unclear in vivo. Belongs to the protein arginine N-methyltransferase family. PRMT7 subfamily. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 2.1.1.126; EC 2.1.1.125; Methyltransferase, protein arginine; Methyltransferase; RNA processing

Chromosomal Location of Human Ortholog: 16q22.1

Cellular Component: nucleoplasm; plasma membrane; nucleolus; cytosol; nucleus

Molecular Function: ribonucleoprotein binding; protein-arginine omega-N asymmetric methyltransferase activity; protein-arginine omega-N monomethyltransferase activity; [myelin basic protein]-arginine N-methyltransferase activity; histone binding; protein-arginine omega-N symmetric methyltransferase activity; S-adenosylmethionine-dependent methyltransferase activity; histone-arginine N-methyltransferase activity

Biological Process: histone methylation; peptidyl-arginine methylation, to symmetrical-dimethyl arginine; establishment and/or maintenance of chromatin architecture; spliceosomal snRNP biogenesis; peptidyl-arginine methylation; genetic imprinting; regulation of transcription, DNA-dependent; transcription, DNA-dependent; DNA methylation during gametogenesis; regulation of protein binding; peptidyl-arginine methylation, to asymmetrical-dimethyl arginine; cell differentiation